Scientia Agricultura Sinica ›› 2008, Vol. 41 ›› Issue (3): 766-771 .doi: 10.3864/j.issn.0578-1752.2008.03.018

• HORTICULTURE • Previous Articles     Next Articles

A Study on Formation and Transmission of n+1 Gametes of Primary Trisomics of Cabbage

  

  1. 河北农业大学园艺学院
  • Received:2006-11-27 Revised:2007-02-06 Online:2008-03-10 Published:2008-03-10

Abstract: Abstract:【Objective】 Transmission rates of n+1 gametes is a important parameter for genetic analysis of trisomics. Determination of n+1 gamete transmission rate of the cabbage trisomics is significant for using this set of trisomics to carry out gene orientation and other genetic studies. 【Method】Each primary trisomics of the cabbage with their diploid parent-9601 was crossed reciprocally, and the hybrid seeds were cultured on MS medium to propagate into clones which were used for chromosome number identification by root tip squash method. The ratio of (2n+1)plants and total plants identified from progenies of 2n+1×2n was denoted as the female n+1 gamete transmission rate, and that from 2n×2n+1 was denoted as the male n+1 gamete transmission rate.【Result】The female n+1 gamete transmission rates were 15.28% for tri-1, 12.68% for tri-2, 12.31% for tri-3, 30.51% for tri-4, 22.81% for tri-5, 7.46% for tri-6, 5.36% for tri-7, 42.37% for tri-8, 9.23% for tri-9. The female n+1 gamete transmission rates were 12.12% for tri-1, 12.33% for tri-2, 7.81% for tri-3, 4.76% for tri-4, 8.93% for tri-5, 10.94% for tri-6, 1.54% for tri-7, 2.94% for tri-8, 13.04% fortri-9.The main affecting factors on male n+1 gamete formation and transmission were the rates of trivalent formation at prophaseⅠand 9/9/10/10 division at anaphaseⅡ, and pollen viability.【Conclusion】n+1 gametes of the cabbage trisomics can transmitted through female and male parents.

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