亚麻快速生长期细胞壁形成相关基因的表达分析

doi:10.3864/j.issn.0578-1752.2017.13.004

Abstract

Abstract: 【Objective】 The development of flax bast cells is separated into two non-overlapping phases, cell elongation and secondary cell wall thickening phases which are splitted by the snap point at rapid-growing phase. Genes of β-glycosyl hydrolyse (LuBGALs) family and Cellulose synthase (LuCESAs) family focused on the role of cell wall formation in flax (Linum usitatissimum) fibres were analyzed in order to gain a deeper insight into the development of cell walls in rapid growth of flax.【Method】The microstructure of the cell walls at snap point (SP) of stem was studied by transmission electron microscope. LuBGALs and LuCESAs were analyzed by quantitative real-time PCR (qRT-PCR) in the bast fiber of stem, leaves and root of flax at 45 days after germination. 【Result】The width of cell walls at top of SP (TOP) was 110 nm. Though they at MID were about 500 nm and BOT about 650 nm under the SP were thicker than theirs in TOP, it was difficult to find different layers in the thicken cell wall. It showed that the cell walls above the SP were undergone cell elongation and below were thickening but not secondary cell walls thickening. The expression of LuBGAL1 at TOP was significantly lower than it’s in MID and BOT, indicating that it was mainly involved in cell wall thickening of fiber cell in the stem. While the expressions of LuBGAL3, LuBGAL6, and LuBGAL9 at TOP were the highest and the followed was MID zone, which showed that those genes mainly worked in cell wall reconstructure and bast fiber elongation. LuBGAL5 was higher at TOP and lower at MID than other genes, which demonstrated that LuBGAL5 played a role in cell wall formation. And the expression of other BGALs genes were low. LuCESA1, LuCESA3, LuCESA7, LuCESA8, LuCESA9, and LuCESA10 highly expressed in TOP zone and were significantly higher than their expression in MID and BOT. The expression of LuCESA3 and LuCESA10 in MID was lower than theirs in BOT and there was no significant difference between MID and BOT of other CESAs genes. The results suggested that these CESA genes played an important role in cell elongation of flax bast fiber combining their expression in different organs. The character of LuSuSy expression indicated that a lot of UDPG was required in cell elongation and thickening of bast fiber. LuXTH4 played a role in cell wall development of flax.【Conclusion】The cell walls of bast fiber had not processed secondary thickening in the stem at fast growth stage. LuBGAL3, LuBGAL5, LuBGAL6, LuBGAL9, LuCESA1, LuCESA3, LuCESA9, and LuCESA10 mainly promoted cell elongation of cell wall in flax. LuBGAL1 played an important role in cell wall thickening. LuSuSy and LuXTH4 also took part in the development of cell wall.

Key words: flax (Linum usitatissimum L.), bast fiber, cell wall formation, gene expression

JIANG HaiXia, GUO DongLiang, LI YuHuan, YAN WenLiang, YANG LiangJie, XIE LiQiong. The Expression of Genes Related to Cell Wall Formation at Fast Growth Stage in Flax (Linum usitatissimum L.)[J].Scientia Agricultura Sinica, 2017, 50(13): 2442-2450.

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References

[1]    GORSHKOVA T A, AGEEVA M, CHEMIKOSOVA S, SALNIKOV V. Tissue-specific processes during cell wall formation in flax fiber. Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology, 2005, 139(1): 88-92.
[2]    ROACH M J, MOKSHINA N Y, BADHAN A, SNEGIREVA A V, HOBSON N, DEYHOLOS M K, GORSHKOVA T A. Development of cellulosic secondary walls in flax fibers requires β-galactosidase. Plant Physiology, 2011, 156(3): 1351-1363.

[3]    MOKSHINA N E, IBRAGIMOVA N N, SALNIKOV V V, AMENITSKII S I, GORSHKOVA T A. Galactosidase of plant fibers with gelatinous cell wall: Identification and localization. Russian Journal of Plant Physiology, 2012, 59(2): 246-254.

[4]    SAMPEDRO J, GIANZO C, IGLESIAS N, GUITI N E, REVILLA G, ZARRA I. AtBGAL10 is the main xyloglucan β-galactosidase in Arabidopsis, and its absence results in unusual xyloglucan subunits and growth defects. Plant Physiology, 2012, 158(3): 1146-1157.

[5]    CHANDRASEKAR B, RA V D H. Beta galactosidases in Arabidopsis and tomato-a mini review. Biochemical Society Transactions, 2016, 44(1): 150-158.

[6]    ZHAO G R, LIU J Y, XIONGMING D U. Molecular cloning and characterization of cotton cDNAs expressed in developing fiber cells. Agricultural and Biological Chemistry, 2001, 65(12): 2789-2793.

[7]    HOBSON N, DEYHOLOS M K. Genomic and expression analysis of the flax (Linum usitatissimum) family of glycosyl hydrolase 35 genes. BMC Genomics, 2013, 14(1): 207-210.

[8]    ROBERT S, MOUILLE G, H FTE H. The mechanism and regulation of cellulose synthesis in primary walls: Lessons from cellulose-deficient Arabidopsis mutants. Cellulose, 2004, 11(3): 351-364.

[9]    TAYLOR N G. Cellulose synthesis in the Arabidopsis secondary cell wall. Cellulose, 2004, 11(3): 329-338.

[10]   FANG L, TIAN R, CHEN J, WANG S, LI X, WANG P, ZHANG T. Transcriptomic analysis of fiber strength in upland cotton chromosome introgression lines carrying different Gossypium barbadense chromosomal segments. PLoS ONE, 2014, 9(4): 220-226.

[11]   LI A, XIA T, XU W, CHEN T, LI X, FAN J, WANG R, FENG S, WANG Y, WANG B, PENG L. An integrative analysis of four CESA isoforms specific for fiber cellulose production between Gossypium hirsutum and Gossypium barbadense. Planta, 2013, 237(6): 1585-1597.

[12]   HAI G, JIA Z, XU W, WANG C, CAO S, LIU J, CHENG Y. Characterization of the Populus PtrCesA4 promoter in transgenic Populus alba × P. glandulosa. Plant Cell, Tissue and Organ Culture, 2016, 124(3): 495-505.

[13]   DJERBI S, LINDSKOG M, ARVESTAD L, STERKY F, TEERI T T. The genome sequence of black cottonwood (Populus trichocarpa) reveals 18 conserved cellulose synthase (CesA) genes. Planta, 2005, 221(5): 739-746.

[14]   KUMAR M, THAMMANNAGOWDA S, BULONE V, CHIANG V, HAN K H, JOSHI C P, MANSFIELD S D, MELLEROWICZ E, SUNDBERG B, TEERI T. An update on the nomenclature for the cellulose synthase genes in Populus. Trends in Plant Science, 2009, 14(5): 248-254.

[15]   SONG D, SHEN J, LI L. Characterization of cellulose synthase complexes in Populus xylem differentiation. New Phytologist, 2010, 187(3): 777-790.

[16] SUZUKI S, LI L G, SUN Y H, CHIANG V L. The cellulose synthase gene superfamily and biochemical functions of xylem-specific cellulose synthase-like genes in Populus trichocarpa. Plant Physiology, 2006, 142(3): 1233-1245.

[17]   WU L, JOSHI C P, CHIANG V L. A xylem-specific cellulose synthase gene from aspen (Populus tremuloides) is responsive to mechanical stress. The Plant Journal, 2000, 22(6): 495-502.

[18]   SAMUGA A, JOSHI C P. A new cellulose synthase gene (PtrCesA2) from aspen xylem is orthologous to Arabidopsis AtCesA7 (irx3) gene associated with secondary cell wall synthesis. Gene, 2002, 296(1/2): 37-44.

[19]   KALLURI U C, JOSHI C P. Isolation and characterization of a new, full-length cellulose synthase cDNA, PtrCesA5 from developing xylem of aspen trees. Journal of Experimental Botany, 2003, 54(390): 2187-2188.

[20]   CHANTREAU M, CHABBERT B, BILLIARD S, HAWKINS S, NEUTELINGS G. Functional analyses of cellulose synthase genes in flax (Linum usitatissimum) by virus-induced gene silencing. Plant Biotechnology Journal, 2015, 13(9): 1312-1324.

[21]   袁红梅, 郭文栋, 赵丽娟, 于莹, 吴建忠, 张利国, 程莉莉, 赵东升, 吴广文, 关凤芝. 亚麻纤维素合酶超基因家族的生物信息学及表达分析. 中国农业科学, 2016, 49(23): 4656-4668.

Yuan H M, Guo W D, Zhao L J, Yu Y, Wu J Z, Zhang L G, Cheng L L, Zhao D S, Wu G W, Guan F Z. Bioinformatics and expression analysis of the cellulose synthase supergene family in flax. Scientia Agricultura Sinica, 2016, 49(23): 4656-4668. (in Chinese)

[22]   KHOTYLEVA L, GALINOUSKY D, ANISIMOVA N, RAISKI A, LEONTIEV V, TITOK V. Cellulose synthase genes that control the fiber formation of flax (Linum usitatissimum L.). Russian Journal of Genetics, 2014, 50(1): 26-34.

[23]   MOKSHINA N, GORSHKOVA T, DEYHOLOS M K. Chitinase-like (CTL) and cellulose synthase (CESA) gene expression in gelatinous- type cellulosic walls of flax (Linum usitatissimum L.) bast fibers. PLoS ONE, 2014, 9(2): e97949.

[24]   HOBSON N, DEYHOLOS M K. Genomic and expression analysis of the flax (Linum usitatissimum) family of glycosyl hydrolase 35 genes. BMC Genomics, 2013, 14(1): 207-210.

[25]   李翔, 陈信波, 邹杰, 龙松华, 邓欣. 亚麻韧皮纤维细胞发育分子生物学研究进展. 湖南农业科学, 2011(9): 4-7.

Li X, Chen X B, Zou J, Long S H, Deng X. Advance in molecular biology of cell development in flax bast. Hunan Agricultural Sciences, 2011(9): 4-7. (in Chinese)

[26]   关凤芝, 张福修, 赵德宝, 倪录. 亚麻纤维发育规律的研究. 中国麻业科学, 1993(1): 14-17.

Guan F Z, Zhang F X, Zhao D B, Ni L. Study on development of flax fiber. China’sFiberCrops, 1993(1): 14-17. (in Chinese)

[27]   李明, 王克荣. 关于亚麻韧皮纤维生长发育阶段划分的探讨. 中国麻业科学, 1996(1): 29.

LI M, Wang K R. Discussion on the growth stage of flax bast. China’s Fiber Crops, 1996(1): 29. (in Chinese)

[28]   GORSHKOVA T, MORVAN C. Secondary cell-wall assembly in flax phloem fibres: Role of galactans. Planta, 2006, 223(2): 149-158.

[29]   SMITH D L, GROSS K C. A family of at least seven beta-galactosidase genes is expressed during tomato fruit development. Plant Physiology, 2000, 123(3): 1173-1183.

[30]   TATEISHI A, INOUE H, YAMAKI S. Fluctuations in activities of three beta-galactosidase isoforms from ripening avocado (Persea americana) fruit and their different activities against its cell wall polysaccharides as substrates. Engei Gakkai Zasshi, 2001, 70(5): 586-592.

[31]   田爱梅, 刘金龙, 曹家树. 植物β-半乳糖苷酶. 中国细胞生物学学报, 2014(5): 703-707.

Tian A M, Liu J L, Cao J S. Beta galactosidase in plants. Chinese Journal of Cell Biology, 2014(5): 703-707. (in Chinese)

[32]   PYDIURA N A, BAYER G Y, GALINOUSKY D V, YEMETS A I, PIRKO Y V, PADVITSKI T A, ANISIMOVA N V, KHOTYLEVA L V, KILCHEVSKY A V, BLUME Y B. Bioinformatic search for cellulose synthase genes in flax (Linum usitatissimum) and their phylogenetic analysis. Cytology and Genetics, 2015, 49(5): 279-287.

[33]   宣云, 赵竑绯, 郭肖颖, 任杰, 王艳, 卢碧芸. 植物细胞壁重构酶木葡聚糖内转糖苷酶/水解酶(XTH)的研究进展. 中国农学通报, 2016(18): 83-88.

Xuan Y, Zhao H F, Guo X Y, Ren J, Wang Y, Lu B Y. Plant cell wall remodeling enzyme xyloglucan endotransglucosylase/hydrolase (XTH). Chinese Agricultural Science Bulletin, 2016(18): 83-88. (in Chinese)

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