Scientia Agricultura Sinica ›› 2015, Vol. 48 ›› Issue (24): 4885-4891.doi: 10.3864/j.issn.0578-1752.2015.24.003

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Crossability Between Brassica napus with Hexaploid AnAnCnCnCoCo and Sclerotinia Resistance in the Hybrids

LIU Yao, DING Yi-juan , WANG Lei , WAN Hua-fang , MEI Jia-qin, QIAN Wei   

  1. College of Agronomy and Biotechnology, Southwest University/Chongqing Rapeseed Engineering & Technology Research Center, Chongqing 400715
  • Received:2015-06-16 Online:2015-12-16 Published:2015-12-16

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Abstract: 【Objective】 It was previously proposed to transfer genetic components from Brassica oleracea into B. napus via the bridge of hexaploid derived from B. napus and B. oleracea. The present study was carried out to assess the potential of the hexaploid strategy in rapeseed improvement by investigating crossability between B. napus and hexaploid and evaluating fertility and sclerotinia resistance in the hybrids.【Method】 The hexaploid AnAnCnCnCoCo was developed by embryo rescue and chromosome doubling from a interspecific cross between B. napus var. Zhongshuang 9 and a wild B. oleracea accession with strong sclerotinia resistance. The crossability between the hexaploid and nine B. napus accessions was studied by investigating the seed setting rate of pollinated flowers, and the seed set from open- and self-pollination of the nine F1 hybrids were checked to reveal the fertility of the F1 generation. The sclerotinia resistance was assessed as well in the nine F1 hybrids to investigate the improving efficiency of targeted trait. 【Result】 The hexaploid was identified to have 20 chromosomes from A genome and 36 chromosomes belonging to C genome, indicating the AnAnCnCnCoCo genome structure of the hexaploid. Genetic components from both Zhongshuang 9 and the wild B. oleracea were identified in the hexaploid. The seed set of hexaploid averaged 5.6 and 8.6 seeds/pod for self- and open- pollination, respectively. The pod setting rate of crosses between hexaploid and nine B. napus genotypes was 69.1%-92.9%, yielding 4.7-10.5 seeds per pod. A significant difference was detected among the nine crosses for both the pod setting rate and the seed set (P＜0.01). The F1 hybrids showed normal pollen fertility but varied seed sets after selfing (11.4-20.7) and open pollination (19.9-26.1 seeds/pod) (P＜0.0001), with a positive correlation (r = 0.67*, P＜0.05) between the selfed- and open- seed sets. Based on stem resistance evaluation across 2013 and 2014, the relative susceptibility of hexaploid to Zhongshuang 9 was 0.47, while the relative susceptibility of the nine B. napus parental lines were 0.94-1.26, with significant differences among genotypes (P＜0.0001). The relative susceptibilities of F1 hybrids were 0.56-1.10, being intermediate between corresponding parental lines and significantly associated with the resistance level of the B. napus parent (r = 0.78*, P＜0.05). Among the nine hybrids, five showed significantly lower relative susceptibilities as compared to their corresponding B. napus parents (P＜0.05), suggesting improved sclerotinia resistance in these F1 hybrids. 【Conclusion】Although the crossability of B. napus with the AnAnCnCnCoCo hexaploid, which has good fertility, was significantly affected by parental B. napus, it is easy to develop F1 hybrids with good fertility and markedly improved performances in the targeted traits.

Key words: Brassica oleracea; Brassica napus, hexaploid AnAnCnCnCoCo;crossability, Sclerotiniasclerotiorum

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