Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (16): 3373-3381.doi: 10.3864/j.issn.0578-1752.2012.16.017

• STORAGE·FRESH-KEEPING·PROCESSING • Previous Articles     Next Articles

Determination of Acrylamide in Kitchen Waste Using Gas Chromatography-Tandem Mass Spectrometry

 CHENG  Jie, SU  Xiao-鸥, ZHANG  Wei, WANG  Rui-Guo, WANG  Pei-Long, LI  Yang   

  1. 中国农业科学院农业质量标准与检测技术研究所，北京100081
  • Received:2011-11-28 Online:2012-08-15 Published:2012-05-31

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Abstract: 【Objective】The objective of this study is to develop an analysis methods of the acrylamide in kitchen waste using gas chromatography-tandem mass spectrometry.【Method】The use of warm deionized water extraction and C18 SPE clean-up or the formic acid extraction and Carrez reagent/MCX clean-up as the sorbented appeared to be a very effective sample preparation method. After derivative reaction, gas chromatography-tandem mass spectrometry was used to detect the sample in the mode of SRM, and the external standard method was used for quantitative analysis. The qualitative ion is m/z 152＞135 and 152＞107 and the quantitative ion is m/z 152＞135. The pressure of gas collision is 15V.【Result】The developed method presented a good linearity from 3 ng•L-1 to 30 ng•L-1 with the correlation coefficient of 0.9984. The RSD of the method was 4.3% and of the quantitative limit was 1.0 ng•g-1. The rate of recovery was 95.3%-108.1%.【Conclusion】The developed method is a very effective method for detecting the acrylamide in kitchen waste.

Key words: gas chromatography-tandem mass, kitchen waste, acrylamide

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