Abstract:

【Objective】 Oxya-specific molecular marker was studied in order to provide a rapid and effective molecular identification method. 【Method】 Oxya-specific RAPD band was screened out from lots of RAPD-PCR results, the specific band was cloned and sequenced, a pair of primer was designed based on homologue region after sequence alignment. PCR was performed by using designed specific primers, genomic DNA from different Oxya species and other Acridoidea species were used as template. 【Result】 A clear and reproductive band about 650 bp amplified from random primer S823 was present in different Oxya species. Sequence analysis showed high nucleotide homology (92.3%-96.6%) among three Oxya species, each sequence determined consisted of 651-667 nucleotides, with G+C content >15% and repetitive A or T regions. Sequence characterized amplified region (SCAR) primers for specific PCR were developed, an expected specific band (550 bp) was obtained by using SCAR primers in seven species of Oxya examined but absent in Pseudoxya diminuta and other Acridoidea species. 【Conclusion】 Oxya-specific SCAR molecular marker was identified, and SCAR primers can be used for rapid identification of Oxya species.

Key words: Oxya, RAPD-PCR, SCAR molecular marker