Abstract: [Objective] This research was conducted to determine the effects of characteristics of cropland eco-environment in seedling stage of wheat (Triticum aestivum L.) inter-planting with paddy and their effects on wheat growth.[ Method] Two different cultivation styles on wheat were included comparatively: wheat inter-planting with paddy, band seeding wheat. [Result] The results showed that the temperature, moisture content and physical character of soil, light intensity were affected significantly by inter-planting with paddy. The temperature around seeds and its diurnal change were lower, but the soil moisture content and the diurnal change of the humidity around seeds was higher. With the bigger bulk density and solid ratio of soil, the penetration resistance of soil was bigger evidently. The light intensity on the face of soil was 4.2% - 6.5% of that on the top of paddy population. Because of the effect of seeds’ uncovering and cropland eco-environment conditions of inter-planting jointly, the ability of water absorption, germination and emergence of seeds was not high comparatively. After emergence, the development and growth of leaves and roots were restrained. The seedlings were not healthy and strong with roots growing downward difficultly, thinner and longer leaves and shorter and little roots. Moreover, photosynthetic power of leaves was not powerful. But roots energy was about 35% stronger. [Conclusion] The seedlings had much probability to be affected badly by the bad conditions. So the developed and improved eco-environment was the key to emergence and healthy and strong seedlings, and benefited the development and growth of wheat to establish rational structure of individual and population, accordingly to have a high yield.