关键词: 小麦, 甲基磺酸乙酯(EMS), 突变体

Abstract:

【Objective】 The mutant library is the basic material of functional genomics. In wheat, this kind of mutant library was rarely reported. In this study, two wheat mutant libraries with EMS were constructed for laying a foundation for wheat functional genomics research. 【Method】 EMS was used as chemical mutagen to build the mutant library with early mature spring wheat Yanzhan 4110. Biological characters and agronomic traits were investigated in M2 generation. Some of mutants identified from M2 plants will be validated through M3 generation. 【Result】 During the growth period after sowing the M2 seeds, all the phenotypic changes of plants were observed and recorded. Phenotype mutants such as seedling habits, leaf appearance, plant height, spike appearance and mature period were obtained. Some of them were related to important agronomic traits and biological characters, specifically, some mutants were rarely observed in natural variation, such as extremely dwarf, with plant height at about 10-15cm. The rate of M2 phenotype mutation was 6.6%. 【Conclusion】 Two libraries of Yanzhan 4110 induced with EMS are ideal mutant libraries and they will be efficiently used in wheat functional genomic research and wheat improvement.

Key words: wheat, ethyl methane sulfonate (EMS), mutant