关键词: 家蚕')">家蚕, 线粒体, 细胞色素C, 细胞凋亡

Abstract:

【Objective】 Cytochrome c plays a key role in the pathway of mitochondria apoptosis. This study on the cloning and protein release of cytochrome c in the apoptosis cells of silkworm can lay a foundation for the apoptosis research of Bombyx mori. 【Method】 Cytochrome c gene was cloned by bioinformation and molecular biology method. Apoptosis of silkworm cells was induced by UVC. The change of the mitochondrial membrane potential and the release of cytochrome c in apoptosis cells of silkworm were detected by flow cytometry and western-blotting, respectively. 【Result】 The cytochrome c gene was cloned in silkworm (Bombyx mori). The gene contains 3 exons and the length of its cDNA is 570 bp and ORF is 324 bp. The deduced protein has 108 aa and has conservative points for cytochrome c and Apaf-1 interaction related to apoptosis in silkworm. After UVC treated BmE-SWU1 cells for 12 h, the mitochondrial membrane potential decreased and cytochrome c was released from mitochondria to cytoplasm. 【Conclusion】 The cytochrome c may release from mitochondria to cytoplasm in the apoptosis cells in silkworm.

Key words: Bombyx mori')">Bombyx mori, mitochondrial, cytochrome c, apoptosis