关键词: 小麦, 白粉病, 微卫星标记, 周98165, 基因定位

Abstract:

【Objective】 Powdery mildew is one of the most important diseases of wheat worldwide. Zhou 98165, a winter wheat provided by wheat research center of Henan Academy of Agricultural Sciences, is a novel line which has highly resistant, high yield and excellent comprehensive agricultural characteristics. Inheritance of powdery mildew resistance gene and mapping of that in Zhou 98165 with simple sequence repeat (SSR) markers were studied to formulate efficient resistance gene and strategies for breeding cultivars resistant to powdery mildew. 【Method】 F1, F2, BC1 populations were constructed on the basis of the hybridization between Zhou 98165 and a susceptible wheat variety Chinese Spring. Powdery mildew strains LY2-1, ZZ1-1 and 02B1 were used for the test the resistance and genetic analysis of those populations with the SSR and EST-SSR primers. The location of this gene on chromosome was confirmed by the materials of monosomic CS. 【Result】 Genetic analysis of Zhou 98165 indicated that the resistance to powdery mildew race 08B1 was controlled by a single dominant karyon gene, temporarily designated as PmHNK. Five SSR markers , Xbarc77, Xgwm547, Xwmc 326,Xgwm299, and Xgwm108 were linked to PmHNK as they were all located on chromosome 3BL. PmHNK, located on 3BL,closely linked to Xgwm299 and Xgwm108 with genetic distances of 4.2 cM and 5.6 cM, respectively.The farthest marker Xbarc77 was 10.6 cM to PmHNK. 【Conclusion】 Genetic analysis and resistance identification with powdery mildew isolates to Zhou98165 combined with SSR markers supports the conclusion that the highly resistance gene PmHNK is a novel gene for powdery mildew in wheat.

Key words: common wheat, powdery mildew, microsatellite marker, Zhou 98165, gene mapping