关键词: 石竹, 小孢子发育, 雄性不育, 石蜡切片

Abstract: 【Objective】In order to study the difference of microsporogenesis between the male-sterile line and fertile line of Chinese pink. 【Method】The microsporogenesis of male sterile and fertile lines in Chinese pink was histologically examined using squashed pollen and paraffin embedded sections. The stable male-sterile line (H-37B) was obtained from one 6 generation inbred lines crossed for several rounds and 2 generations’ backcross. 【Result】Results showed that mature pollen was developed through the stage of the initiation of the sporogenous cell, microsporocyte, and tetrad period, microspore formation and eventually developed into normal pollen. Compared with fertile pollens, the inhibition of microsporogenesis of male sterility occurred at the tetrad stage to uninucleate stage. And the irregular triangle contour and hollow interior of tetrad-microspore were observed. In uninuclear stage, the tapetum layer cells retained in the home position and expanded and crimpy microspore stopped development. There were less pollen and almost all the pollens were empty and lack of vitality. 【Conclusion】This male-sterile line inherits stably and will be a useful material for breeding.

Key words: Dianthus chinensis L., microsporogenesis, paraffin section, cytology