基于转录组和蛋白质组揭示库尔勒香梨萼片脱落的分子机制

doi:10.3864/j.issn.0578-1752.2025.18.012

摘要/Abstract

摘要：

【目的】库尔勒香梨萼片脱落有助于其果实品质提升。通过多组学联合分析，探究库尔勒香梨萼片脱落的分子机制，明确萼片脱落过程中的差异表达基因和蛋白，以及参与的代谢通路和信号传导途径，丰富库尔勒香梨萼片脱落机理，为进一步研究库尔勒香梨萼片脱落机制，以及生产上利用生理、分子等技术调控库尔勒香梨萼片脱落提供理论依据。【方法】以库尔勒香梨为研究材料，选取萼片离区形成关键期脱萼果离区和宿萼果离区对应部位为研究对象，进行转录组和蛋白质组的测序与联合分析，利用多种生物信息学工具筛选差异表达基因与蛋白，重点分析与脱落相关的基因与蛋白，明确其参与的代谢通路和信号传导途径，最后通过实时荧光定量PCR（qRT-PCR）验证差异表达基因。【结果】通过转录组分析共获得393个差异表达基因，主要富集在细胞壁生物合成、植物型细胞壁组织或生物合成、植物型细胞壁生物合成、植物型次生细胞壁生物合成、苯丙烷类代谢过程等，以及代谢途径、次生代谢物的生物合成及戊糖和葡糖醛酸相互转化等通路，表明差异表达基因大多参与细胞壁的代谢。通过蛋白质组分析共获得256个差异表达蛋白，联合分析结果显示，差异表达基因和蛋白主要富集在苯丙烷生物合成、各种植物次生代谢产物的生物合成、植物激素信号转导、戊糖和葡糖醛酸相互转化等通路，筛选出与植物激素（如乙烯响应转录因子、吲哚-3-乙酸O-甲基转移酶基因）和细胞壁相关基因（如果胶裂解酶基因、果胶酯酶基因、多聚半乳糖醛酸酶基因、过氧化物酶基因），它们在库尔勒香梨萼片脱落过程中发挥着重要作用。此外，对所筛选的10个差异表达基因进行实时荧光定量PCR验证，其表达趋势与测序结果一致。【结论】从基因转录与蛋白质表达层面初步确定了库尔勒香梨萼片脱落的调控机制，筛选出植物激素和细胞壁相关的基因与蛋白，明确了库尔勒香梨萼片脱落过程主要涉及苯丙烷生物合成、植物激素信号转导、戊糖和葡糖醛酸相互转化通路。

关键词: 库尔勒香梨, 萼片, 离区, 转录组, 蛋白质组

Abstract:

【Objective】Calyx abscission in Korla Xiangli contributes to the improvement of fruit quality. Through multi-omics integrated analysis, the molecular mechanism of calyx abscission in Korla Xiangli was explored. The differentially expressed genes and proteins during the abscission process, as well as the involved metabolic pathways and signal transduction pathways were identified. This enriched the understanding of the abscission mechanism of calyx and provided a theoretical basis for further research on the abscission mechanism of calyx in Korla Xiangli. It also laid the foundation for the application of physiological and molecular techniques in regulating calyx abscission in Korla Xiangli in production. 【Method】Using Korla Xiangli pear as the research material, the clayx abscission zone of decalyx fruits and the corresponding parts of the abscission zone of persistent calyx fruits at the critical period of abscission zone formation were selected as research objects. Transcriptome and proteome sequencing, along with integrated analysis were performed. Multiple bioinformatics tools were employed to screen differentially expressed genes and proteins. The genes and proteins related to abscission were analyzed in detail, and the metabolic pathways and signal transduction pathways they participated in were identified. Finally, the differentially expressed genes were verified by quantitative real-time PCR (qRT-PCR). 【Result】A total of 393 differentially expressed genes were obtained through transcriptome analysis. These genes were mainly enriched in cell wall biosynthesis, plant-type cell wall organization or biogenesis, plant-type cell wall biosynthesis, plant-type secondary cell wall biosynthesis, phenylpropanoid metabolic processes, etc., as well as metabolic pathways, biosynthesis of secondary metabolites, and mutual Pentose and glucuronate interconversions, which indicated that most differentially expressed genes were involved in cell wall metabolism. A total of 256 differentially expressed proteins were obtained through proteome analysis. The integrated analysis showed that the differentially expressed genes and proteins were mainly enriched in pathways of plant hormone signal transduction, pentose and glucuronate interconversions, zeatin biosynthesis, and phenylpropanoid biosynthesis. Plant hormone-related genes (e.g., ethylene-responsive transcription factors, indole-3-acetic O-methyltransferase gene) and genes/proteins involved in cell wall degradation (e.g., pectin lyase gene, pectin esterase gene, polygalacturonase gene, peroxidase gene) were screened out, playing critical roles in calyx abscission of Korla Xiangli. In addition, the expression trends of 10 differentially expressed genes verified by qRT-PCR were consistent with the sequencing results. 【Conclusion】The regulation mechanism of calyx abscission in Korla Xiangli was determined from the levels of gene transcription and protein expression. Genes and proteins related to plant hormones and cell wall degrading enzymes were screened out, and clarified that the calyx abscission process of Korla Xiangli mainly involved in pathways such as phenylpropanoid biosynthesis, plant hormone signal transduction, pentose and glucuronate interconversions.

Key words: Korla Xiangli, calyx, abscission zone, transcriptome, proteome

林彦, 郑玲玲, 田嘉, 温玥, 陈晨, 王磊. 基于转录组和蛋白质组揭示库尔勒香梨萼片脱落的分子机制[J]. 中国农业科学, 2025, 58(18): 3744-3765.

LIN Yan, ZHENG LingLing, TIAN Jia, WEN Yue, CHEN Chen, WANG Lei. Based on Transcriptomics and Proteomics to Provide Insights into the Molecular Mechanisms of Calyx Abscission in Korla Xiangli[J]. Scientia Agricultura Sinica, 2025, 58(18): 3744-3765.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2025.18.012

https://www.chinaagrisci.com/CN/Y2025/V58/I18/3744

图/表 24

图1

表1

表2

图2

图3

图4

图5

表3

差异表达基因前20个GO功能富集分析"

分类 Category	GO编号 GO number	条目 Term	差异基因数量 DEGs number	校正后P值 Corrected P-value
生物过程 Biological process	GO:0009834	植物型次生细胞壁生物合成 Plant-type secondary cell wall biogenesis	36	2.06×10^-14
	GO:0042546	细胞壁生物合成 Cell wall biogenesis	48	5.28×10^-37
	GO:0071669	植物型细胞壁组织或生物合成 Plant-type cell wall organization or biogenesis	46	9.20×10^-31
	GO:0009832	植物型细胞壁生物合成 Plant-type cell wall biogenesis	38	1.17×10^-30
	GO:0045492	木聚糖生物合成过程 Xylan biosynthetic process	20	8.92×10^-26
	GO:0044036	细胞壁大分子代谢过程 Cell wall macromolecule metabolic process	32	4.63×10^-24
	GO:0010383	细胞壁多糖代谢过程 Cell wall polysaccharide metabolic process	28	3.96×10^-21
	GO:0009698	苯丙烷类代谢过程 Phenylpropanoid metabolic process	35	3.24×10^-22
	GO:0070589	细胞组分大分子生物合成过程 Cellular component macromolecule biosynthetic process	23	9.34×10^-22
	GO:0044038	细胞壁大分子生物合成过程 Cell wall macromolecule biosynthetic process	23	9.34×10^-22
	GO:0010383	细胞壁多糖代谢过程 Cell wall polysaccharide metabolic process	28	3.96×10^-21
	GO:0045491	木聚糖代谢过程 Xylan metabolic process	22	4.68×10^-21
	GO:0010410	半纤维素代谢过程 Hemicellulose metabolic process	24	3.22×10^-20
	GO:0033692	细胞多糖生物合成过程 Cellular polysaccharide biosynthetic process	30	3.51×10^-19
	GO:0070592	多糖生物合成过程 Polysaccharide biosynthetic process	23	1.63×10^-22
	GO:0046274	木质素分解代谢过程 Lignin catabolic process	15	9.74×10^-18
	GO:0046271	苯丙烷类分解代谢过程 Phenylpropanoid catabolic process	15	9.74×10^-18
分子功能 Molecular function	GO:0016682	氧化还原酶活性，以二酚及相关物质为供体、氧为受体 Oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor	18	2.21×10^-18
	GO:0052716	对苯二酚﹕氧氧化还原酶活性 Hydroquinone﹕oxygen oxidoreductase activity	15	3.05×10^-18
	GO:0016679	氧化还原酶活性，以二酚及相关物质为供体 Oxidoreductase activity, acting on diphenols and related substances as donors	18	1.46×10^-17

表3

表4

图6

图7

图8

表5

表6

图9

图10

图11

表7

图12

表8

图13

图14

图15

图16

参考文献 41

[1]	田雯, 张校立, 陈励坤, 何临梓, 王永鹏. 库尔勒香梨研究进展. 分子植物育种, 2024, 22(16): 5459-5468.
	TIAN W, ZHANG X L, CHEN L K, HE L Z, WANG Y P. Research progress of Korla Xiangli. Molecular Plant Breeding, 2024, 22(16): 5459-5468. (in Chinese)
[2]	高启明, 李疆, 李阳. 库尔勒香梨研究进展. 经济林研究, 2005, 23(1): 79-82.
	GAO Q M, LI J, LI Y. Literature review of researches on ‘Korla Xiangli’. Economic Forest Researches, 2005, 23(1): 79-82. (in Chinese)
[3]	何子顺, 牛建新, 邵月霞. 库尔勒香梨果实萼片脱落与宿存研究概述. 中国果菜, 2006, 26(2): 10-11.
	HE Z S, NIU J X, SHAO Y X. General review of study on the Calyx leaving from fruit and persistent Calyx of Korla Xiangli. China Fruit & Vegetable, 2006, 26(2): 10-11. (in Chinese)
[4]	王翔, 陈晓博, 李爱丽, 毛龙. 植物器官脱落分子生物学研究进展. 作物学报, 2009, 35(3): 381-387. doi: 10.3724/SP.J.1006.2009.00381
	WANG X, CHEN X B, LI A L, MAO L. Advances in molecular biology study of plant organ abscission. Acta Agronomica Sinica, 2009, 35(3): 381-387. (in Chinese) doi: 10.3724/SP.J.1006.2009.00381
[5]	张羽云, 何永梅, 杜婷婷, 张晶晶, 周苑博, 武树义, 范厚东, 裴海霞. 植物器官脱落研究进展. 内蒙古科技大学学报, 2021, 40(3): 209-216.
	ZHANG Y Y, HE Y M, DU T T, ZHANG J J, ZHOU Y B, WU S Y, FAN H D, PEI H X. Research progress of plant organ abscission. Journal of Inner Mongolia University of Science and Technology, 2021, 40(3): 209-216. (in Chinese)
[6]	TAYLOR J E, WHITELAW C A. Signals in abscission. New Phytologist, 2001, 151(2): 323-340.
[7]	GULFISHAN M, JAHAN A, AHMAD BHAT T, SAHAB D. Plant senescence and organ abscission. Senescence Signalling and Control in Plants, Amsterdam: Elsevier, 2019: 255-272.
[8]	邵白俊杰. 乙烯诱导库尔勒香梨萼片脱落过程中细胞程序性死亡特征及其调控信号研究[D]. 乌鲁木齐: 新疆农业大学, 2023.
	SHAO B. Study on the characteristics of programmed cell death and its regulatory signals during ethylene-induced sepal abscission in Korla Xiangli[D]. Urumqi: Xinjiang Agricultural University, 2023. (in Chinese)
[9]	MISHRA A, KHARE S, TRIVEDI P K, NATH P. Ethylene induced cotton leaf abscission is associated with higher expression of cellulase (GhCel1) and increased activities of ethylene biosynthesis enzymes in abscission zone. Plant Physiology and Biochemistry, 2008, 46(1): 54-63. doi: 10.1016/j.plaphy.2007.09.002 pmid: 17964177
[10]	LI C Q, ZHAO M L, MA X S, WEN Z X, YING P Y, PENG M J, NING X P, XIA R, WU H, LI J G. The HD-Zip transcription factor LcHB2 regulates Litchi fruit abscission through the activation of two cellulase genes. Journal of Experimental Botany, 2019, 70(19): 5189-5203. doi: 10.1093/jxb/erz276 pmid: 31173099
[11]	ZHENG L L, WEN Y, LIN Y, TIAN J, SHAOBAI J J, HAO Z C, WANG C F, SUN T Y, WANG L, CHEN C. Phytohormonal dynamics in the abscission zone of Korla fragrant pear during Calyx abscission: A visual study. Frontiers in Plant Science, 2024, 15: 1452072.
[12]	ZENG N B, YANG Z J, ZHANG Z F, HU L X, CHEN L. Comparative transcriptome combined with proteome analyses revealed key factors involved in alfalfa (Medicago sativa) response to waterlogging stress. International Journal of Molecular Sciences, 2019, 20(6): 1359.
[13]	PEI M S, NIU J X, LI C J, CAO F J, QUAN S W. Identification and expression analysis of genes related to Calyx persistence in Korla fragrant pear. BMC Genomics, 2016, 17: 132. doi: 10.1186/s12864-016-2470-3 pmid: 26911295
[14]	MA L, ZHOU L, QUAN S W, XU H, YANG J P, NIU J X. Integrated analysis of mRNA-seq and miRNA-seq in Calyx abscission zone of Korla fragrant pear involved in Calyx persistence. BMC Plant Biology, 2019, 19(1): 192. doi: 10.1186/s12870-019-1792-0 pmid: 31072362
[15]	QI X X, WU J, WANG L F, LI L T, CAO Y F, TIAN L M, DONG X G, ZHANG S L. Identifying the candidate genes involved in the Calyx abscission process of ‘Kuerlexiangli’ (Pyrus sinkiangensis Yu) by digital transcript abundance measurements. BMC Genomics, 2013, 14(1): 727.
[16]	徐航, 全绍文, 马丽, 周丽, 牛建新. 库尔勒香梨PsiERF基因的克隆和表达分析. 新疆农业科学, 2018, 55(9): 1616-1625. doi: 10.6048/j.issn.1001-4330.2018.09.006
	XU H, QUAN S W, MA L, ZHOU L, NIU J X. Molecular cloning of the PsiERF gene and its expression in Korla Xiangli. Xinjiang Agricultural Sciences, 2018, 55(9): 1616-1625. (in Chinese)
[17]	WANG B H, SUN X X, DONG F Y, ZHANG F, NIU J X. Cloning and expression analysis of an MYB gene associated with Calyx persistence in Korla fragrant pear. Plant Cell Reports, 2014, 33(8): 1333-1341.
[18]	YANG S J, VANDERBELD B, WAN J X, HUANG Y F. Narrowing down the targets: Towards successful genetic engineering of drought- tolerant crops. Molecular Plant, 2010, 3(3): 469-490.
[19]	GONG X, BAO J P, CHEN J, QI K J, XIE Z H, RUI W K, HAO G W, SHIRATAKE K, KHANIZADEH S, ZHANG S L, et al. Candidate proteins involved in the Calyx abscission process of ‘Kuerlexiangli’ (Pyrus sinkiangensis Yu) identified by iTRAQ analysis. Acta Physiologiae Plantarum, 2020, 42(7): 112.
[20]	蒋可人, 马峥, 郑航, 刘小军. 转录组与蛋白质组整合分析在生物学研究中的应用. 生物技术通报, 2018, 34(12): 50-55. doi: 10.13560/j.cnki.biotech.bull.1985.2017-0929
	JIANG K R, MA Z, ZHENG H, LIU X J. Review on the application of integrated transcriptome and proteome analysis in biology. Biotechnology Bulletin, 2018, 34(12): 50-55. (in Chinese)
[21]	郝志超, 温玥, 田嘉, 邵白俊杰, 杨丁花, 张峰. 库尔勒香梨萼片脱落与离区不同部位植物激素的关系. 植物生理学报, 2022, 58(7): 1369-1380.
	HAO Z C, WEN Y, TIAN J, SHAO B J J, YANG D H, ZHANG F. Relationship between calyx abscission and phytohormones in different parts of abscission zone of Korla Xiangli. Plant Physiology, 2022, 58(7): 1369-1380. (in Chinese)
[22]	郝志超. ‘库尔勒香梨’萼片脱落的激素变化及对果实品质的影响[D]. 乌鲁木齐: 新疆农业大学, 2022.
	HAO Z C. Hormone changes in sepal abscission of ' Korla Xiangli' and its effect on fruit quality[D]. Urumqi: Xinjiang agricultural university, 2022. (in Chinese)
[23]	BUCHFINK B, XIE C, HUSON D H. Fast and sensitive protein alignment using DIAMOND. Nature Methods, 2014, 12(1): 59-60.
[24]	孙晓霞, 牛建新, 王博慧, 裴茂松, 李陈静, 曹福军. ‘库尔勒香梨’脱萼组与宿萼组样品差异表达基因的筛选. 果树学报, 2015, 32(6): 1020-1027, 1314.
	SUN X X, NIU J X, WANG B H, PEI M S, LI C J, CAO F J. Screening of differentially expressed genes in‘Korla Xiangli’flower samples with persistent or deciduous Calyx. Journal of Fruit Science, 2015, 32(6): 1020-1027, 1314. (in Chinese)
[25]	CHERSICOLA M, KLADNIK A, ŽNIDARIČ M T, MRAK T, GRUDEN K, DERMASTIA M. 1-aminocyclopropane-1-carboxylate oxidase induction in tomato flower pedicel phloem and abscission related processes are differentially sensitive to ethylene. Frontiers in Plant Science, 2017, 8: 464. doi: 10.3389/fpls.2017.00464 pmid: 28408916
[26]	NAKANO T, SUZUKI K, FUJIMURA T, SHINSHI H. Genome-wide analysis of the ERF gene family in Arabidopsis and rice. Plant Physiology, 2006, 140(2): 411-432.
[27]	AGUSTÍ J, MERELO P, CERCÓS M, TADEO F R, TALÓN M. Ethylene-induced differential gene expression during abscission of Citrus leaves. Journal of Experimental Botany, 2008, 59(10): 2717-2733. doi: 10.1093/jxb/ern138 pmid: 18515267
[28]	NAKANO T, FUJISAWA M, SHIMA Y, ITO Y. The AP2/ERF transcription factor SlERF52 functions in flower pedicel abscission in tomato. Journal of Experimental Botany, 2014, 65(12): 3111-3119. doi: 10.1093/jxb/eru154 pmid: 24744429
[29]	SINGH P, BHARTI N, SINGH A P, TRIPATHI S K, PANDEY S P, CHAUHAN A S, KULKARNI A, SANE A P. Petal abscission in fragrant roses is associated with large scale differential regulation of the abscission zone transcriptome. Scientific Reports, 2020, 10: 17196. doi: 10.1038/s41598-020-74144-3 pmid: 33057097
[30]	MEIR S, PHILOSOPH-HADAS S, RIOV J, TUCKER M L, PATTERSON S E, ROBERTS J A. Re-evaluation of the ethylene- dependent and-independent pathways in the regulation of floral and organ abscission. Journal of Experimental Botany, 2019, 70(5): 1461-1467.
[31]	ESTORNELL L H, AGUSTÍ J, MERELO P, TALÓN M, TADEO F R. Elucidating mechanisms underlying organ abscission. Plant Science, 2013, 199: 48-60.
[32]	QIN G, GU H, ZHAO Y. Regulation of auxin homeostasis and plant development by an indole-3-acetic acid carboxyl methyltransferase in Arabidopsis. Plant Cell, 2005, 17: 2693-2704.
[33]	LIANG Y, JIANG C Y, LIU Y, GAO Y R, LU J Y, AIWAILI P, FEI Z J, JIANG C Z, HONG B, MA C, et al. Auxin regulates sucrose transport to repress petal abscission in rose (Rosa hybrida). The Plant Cell, 2020, 32(11): 3485-3499. doi: 10.1105/tpc.19.00695 pmid: 33830255
[34]	GAO Y R, LIU C, LI X D, XU H Q, LIANG Y, MA N, FEI Z J, GAO J P, JIANG C Z, MA C. Transcriptome profiling of petal abscission zone and functional analysis of an Aux/IAA family gene RhIAA16 involved in petal shedding in rose. Frontiers in Plant Science, 2016, 7: 1375.
[35]	仇志浪. 贵州甜樱桃异常生理落果的分子机制[D]. 贵阳: 贵州大学, 2021.
	QIU Z L. Molecular mechanism of abnormal physiological fruit drop of sweet cherry in Guizhou[D]. Guiyang: Guizhou University, 2021. (in Chinese)
[36]	KIM J, SUNDARESAN S, PHILOSOPH-HADAS S, YANG R H, MEIR S, TUCKER M L. Examination of the abscission-associated transcriptomes for soybean, tomato, and Arabidopsis highlights the conserved biosynthesis of an extensible extracellular matrix and boundary layer. Frontiers in Plant Science, 2015, 6: 1109. doi: 10.3389/fpls.2015.01109 pmid: 26697054
[37]	刘化禹, 娄爽, 秦栋, 张妍, 谢佳璇, 霍俊伟. 蓝果忍冬果柄离区形成中内源激素含量与细胞壁相关酶活性的变化特征. 西北植物学报, 2019, 39(1): 110-120.
	LIU H Y, LOU S, QIN D, ZHANG Y, XIE J X, HUO J W. Characteristics of endogenous hormones and cell wall-related enzymes activities during formation of carpopodium abscission zone in blue honeysuckle. Acta Botanica Boreali-Occidentalia Sinica, 2019, 39(1): 110-120. (in Chinese)
[38]	王雪. 观赏海棠果实脱落相关酶活测定及转录组分析[D]. 秦皇岛: 河北科技师范学院, 2021.
	WANG X. Determination of enzyme activity and transcriptome analysis related to fruit abscission of ornamental crabapple[D]. Qinhuangdao: Hebei Normal University of Science & Technology, 2021. (in Chinese)
[39]	吴建阳, 刘丽琴, 石胜友, 李伟才, 郑雪文, 魏永赞. 龙眼多聚半乳糖醛酸酶基因DlPG1表达模式与幼果脱落关系研究. 中国南方果树, 2017, 46(3): 14-19.
	WU J Y, LIU L Q, SHI S Y, LI W C, ZHENG X W, WEI Y Z. Preliminary study on the relationship between expression of clade E PGGene and fruitlet abscission in Longan. South China Fruits, 2017, 46(3): 14-19. (in Chinese)
[40]	PACHECO J M, RANOCHA P, KASULIN L, FUSARI C M, SERVI L, APTEKMANN A A, GABARAIN V B, PERALTA J M, BORASSI C, MARZOL E, et al. Apoplastic class III peroxidases PRX62 and PRX69 promote Arabidopsis root hair growth at low temperature. Nature Communications, 2022, 13: 1310.
[41]	SINGH A P, TRIPATHI S K, NATH P, SANE A P. Petal abscission in rose is associated with the differential expression of two ethylene- responsive xyloglucan endotransglucosylase/hydrolase genes, RbXTH1 and RbXTH2. Journal of Experimental Botany, 2011, 62(14): 5091-5103.

基因编号 Gene ID	正向引物 Forward primer (5′-3′)	反向引物 Reverse primer (5′-3′)
Psin03G014400	GGACAAAAGATCAGCAGCCTAC	ACCTGAGAAAGCTTGCCAAG
Psin05G015600	TGCAGCTGAAATTCGTGACC	CCTCAGCTGTCTCAAATGTGC
Psin01G012900	TCTCTTCTTCGGCTCCACTTC	AAATCCCCGTGCAGAATTCC
Psin03G003950	ACGACAACCTTGCACCTTTG	TTTGATCACTGTGGAGGAGACC
Psin12G002990	ACAGCCTAACAGCAAACCAG	TCAGGCCAACAGCAGAAAAC
Psin01G002910	TGCTTGATGACACACCAACC	AAGAAACAACTCCCGGACAC
Psin01G014890	ACTTTCGACAATGGCGGTTC	GCTCAATGACTCCTTTGGTTCC
Psin10G013480	AACAATTTGGGGGTCAAGGG	TCAGTGCAAGTAACGTCGTC
Psin03G018850	GTTCCATGTATCCTTGCAACGG	AACAACTTGTCGGCTGAACC
Psin02G000220	GTTCCATGTATCCTTGCAACGG	AACAACTTGTCGGCTGAACC

样品 Sample	原始序列数 Raw reads	有效序列数 Valid reads	有效碱基数 Valid bases	基因组比对序列数 Reads mapped	Q20 (%)	Q30 (%)
T-L-1	53100666	51652274	7.75	47429147(91.82%)	97.70	93.25
T-L-2	49583548	48231834	7.23	44281991(91.81%)	97.62	93.07
T-L-3	50550552	49112684	7.37	45132315(91.90%)	97.58	92.97
S-LD-1	59022886	57275596	8.59	52859617(92.29%)	97.76	93.44
S-LD-2	64312592	62558674	9.38	57688769(92.22%)	97.70	93.28
S-LD-3	52419448	51073616	7.66	47107851(92.24%)	97.77	93.43

分类 Category	通路编号 Path number	途径 Pathway	差异基因数量 DEGs number	校正后P值 Corrected P-value
代谢 Metabolism	ko01100	代谢途径 Metabolic pathways	64	5.82×10^-7
	ko00040	戊糖和葡萄糖醛酸相互转化 Pentose and glucuronate interconversions	12	7.32×10^-7
	ko01110	次生代谢物的生物合成 Biosynthesis of secondary metabolites	34	1.39×10^-3
	ko00909	倍半萜和三萜生物合成 Sesquiterpenoid and triterpenoid biosynthesis	4	1.92×10^-3
	ko00073	角质、木栓质和蜡的生物合成 Cutin, suberine and wax biosynthesis	4	2.28×10^-3
	ko00908	玉米素生物合成 Zeatin biosynthesis	3	2.51×10^-3
	ko00520	氨基糖和核苷酸糖代谢 Amino sugar and nucleotide sugar metabolism	7	2.91×10^-3
	ko00940	苯丙烷生物合成 Phenylpropanoid biosynthesis	8	1.16×10^-2

分类 Category	GO编号 GO number	条目 Term	差异蛋白数量 DEPs number	校正后P值 Corrected P-value
生物过程 Biological process	GO:0042744	过氧化氢分解代谢过程 Hydrogen peroxide catabolic process	7	1.17×10^-5
	GO:0006979	对氧化应激的响应 Response to oxidative stress	7	8.96×10^-5
	GO:0046274	木质素分解代谢过程 Lignin catabolic process	4	9.21×10^-5
	GO:0009627	系统获得性抗性 Systemic acquired resistance	3	5.19×10^-4
	GO:0009734	生长素激活的信号通路 Auxin-activated signaling pathway	5	1.80×10^-3
	GO:0051726	细胞周期调控 Regulation of cell cycle	2	4.49×10^-3
	GO:0006865	氨基酸运输 Amino acid transport	3	6.44×10^-3
	GO:0030036	肌动蛋白细胞骨架组织 Actin cytoskeleton organization	2	6.64×10^-3
	GO:0045492	木聚糖生物合成过程 Xylan biosynthetic process	2	9.16×10^-3
	GO:0000139	高尔基体膜 Golgi membrane	7	1.02×10^-2
细胞组分 Cellular component	GO:0004601	过氧化物酶活性 Peroxidase activity	7	2.83×10^-5
	GO:0005576	细胞外区域 Extracellular region	12	3.23×10^-5
	GO:0048046	质外体 Apoplast	6	2.96×10^-4
	GO:0005667	转录调节复合物 Transcription regulator complex	2	1.21×10^-3
分子功能 Molecular function	GO:0020037	血红素结合 Heme binding	12	4.82×10^-5
	GO:0052716	对苯二酚﹕氧氧化还原酶活性 Hydroquinone﹕oxygen oxidoreductase activity	4	7.60×10^-5
	GO:0015293	同向转运体活性 Symporter activity	4	3.15×10^-3
	GO:0008194	UDP-糖基转移酶活性 UDP-glycosyltransferase activity	6	4.10×10^-3
	GO:0071702	有机物质运输 Organic substance transport	2	4.49×10^-3
	GO:0005504	脂肪酸结合 Fatty acid binding	2	8.30×10^-3

分类 Category	通路编号 Path number	途径 Pathway	差异蛋白数量 DEPs number	校正后P值 Corrected P-value
代谢 Metabolism	ko00940	苯丙烷生物合成 Phenylpropanoid biosynthesis	9	1.44×10^-4
	ko00999	多种植物次生代谢物的生物合成 Biosynthesis of various plant secondary metabolites	5	8.52×10^-3
	ko00561	甘油酯代谢 Glycerolipid metabolism	5	4.34×10^-2
	ko00908	玉米素生物合成 Zeatin biosynthesis	1	1.86×10^-1
基因信息处理 Genetic information processing	ko03410	碱基切除修复 Base excision repair	3	4.96×10^-2
基因信息处理 Genetic information processing	ko03030	DNA复制 DNA replication	4	1.54×10^-2