基于介质阻挡放电低温等离子体分析储藏稻谷高温胁迫下酚类代谢规律

doi:10.3864/j.issn.0578-1752.2024.06.012

中国农业科学 ›› 2024, Vol. 57 ›› Issue (6): 1180-1190.doi: 10.3864/j.issn.0578-1752.2024.06.012

基于介质阻挡放电低温等离子体分析储藏稻谷高温胁迫下酚类代谢规律

侯帅¹(), 张祎佳¹, 周丹丹², 马飞洋¹, 王大鹏¹, 赵思琪¹, 丁超¹, 刘强¹()

¹ 南京财经大学食品科学与工程学院/江苏省现代粮食流通与安全协同创新中心/江苏高校粮油质量安全控制及深加工重点实验室，南京 210023
² 南京林业大学轻工与食品学院，南京 210037

收稿日期:2023-10-07 接受日期:2024-01-24 出版日期:2024-03-25 发布日期:2024-03-25
通信作者:
刘强，E-mail：qiangliu@nufe.edu.cn
联系方式: 侯帅，E-mail：19851780923@163.com。
基金资助:
国家自然科学基金青年基金(32202132); 中国科协青年人才托举工程(2022NRC001); 江苏省高校优势学科建设工程资助项目(PAPD)

Analysis of the Effect of Dielectric Barrier Discharge Cold Plasma on Phenolic Metabolism of Stored Paddy Rice Under High Temperature Stress

HOU Shuai¹(), ZHANG YiJia¹, ZHOU DanDan², MA FeiYang¹, WANG DaPeng¹, ZHAO SiQi¹, DING Chao¹, LIU Qiang¹()

¹ College of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety/Jiangsu Key Laboratory of Quality Control and Further Processing of Cereals and Oil, Nanjing 210023
² College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037

Received:2023-10-07 Accepted:2024-01-24 Published:2024-03-25 Online:2024-03-25

摘要/Abstract

摘要：

【目的】探讨介质阻挡放电低温等离子体（DBD-CP）处理对储藏稻谷高温胁迫下酚类代谢规律的影响。【方法】模拟夏季高温35 ℃条件下，以新鲜偏高水分稻谷（粳稻，16.0%水分含量，湿基水分）为原料，分析DBD-CP处理对储藏稻谷L^*、a^*、b^*、黄度指数（YI）、活性氧以及丙二醛（MDA）等品质指标的影响，探讨储藏过程中总酚和总黄酮成分的变化规律，并结合抗氧化活性ABTS^＋、DPPH自由基抑制率以及铁离子还原能力等多重验证，联合苯丙烷代谢关键限速酶的活性变化，共同揭示DBD-CP处理下的储藏稻谷酚类代谢规律。【结果】经DBD-CP处理后，稻谷储藏过程中颜色稳定性得到显著提升，对应储藏60 d后的处理组黄变指数显著降低了12.6%，超氧阴离子、过氧化氢以及MDA含量在储藏20 d时分别相对降低14.8%、41.6%和21.6%，对应的新鲜偏高水分稻谷劣变速率显著受到抑制；此外，在高温胁迫储藏期间，处理组样本总酚及总黄酮含量同步增加（P<0.05），最大上升幅度分别为对照组的1.23和1.34倍，对应样本的抗氧化特性和铁离子还原能力均得到显著提升（P<0.05）；结合苯丙烷代谢关键限速酶的活性分析，对应处理后苯丙氨酸解氨酶（PAL）、肉桂酸-4-羟化酶（C4H）和查尔酮合酶（CHS）活性显著增强，上升幅度在1.71—2.28倍；相关性分析进一步证实，高温胁迫下偏高水分稻谷酚类物质含量与PAL、C4H和CHS酶活性密切关联（P<0.05），与籽粒内部活性氧以及丙二醛含量显著负相关（P<0.05）。【结论】介质阻挡放电低温等离子体处理可以通过激活苯丙烷代谢促进偏高水分稻谷籽粒内部酚类合成速率，加强组织抗氧化特性并缓解膜质过氧化和自由基的积累，进而延缓稻谷在高温胁迫储藏环境下的品质劣变。

关键词: 稻谷, 介质阻挡放电低温等离子体, 苯丙烷代谢, 酚类物质, 抗氧化性, 储藏

Abstract:

【Objective】 The aim of his study was to explore the impact of dielectric barrier discharge cold plasma (DBD-CP) treatment on the phenolic metabolism of stored rice under high-temperature stress.【Method】 Under simulated summer high- temperature conditions (35 ℃), fresh high-moisture rice (Japonica rice, 16.0% moisture content on a wet basis) was used as the raw material, the effects of DBD-CP treatment on quality parameters such as L^*, a^*, b^*, yellowness index (YI), reactive oxygen species, and malondialdehyde (MDA) in stored rice were analyzed. Additionally, the study aims to analyze the changes in total phenols, total flavonoids, and antioxidant activities during the storage process. The investigation would involve multiple validations, including ABTS⁺ and DPPH radical scavenging activities, ferric ion reducing power, and the activity changes of key rate-limiting enzymes in phenylpropane metabolism. The ultimate goal was to explore the impact of DBD-CP treatment on the phenolic metabolism of stored rice. 【Result】 After DBD-CP treatment, the color stability of rice during storage significantly improved. The yellowing index of the treatment group decreased significantly by 12.6% after 60 days of storage. After 20 days of storage, the levels of superoxide anion, hydrogen peroxide, and MDA decreased by 14.8%, 41.6%, and 21.6%, respectively, and the deterioration rate of fresh high-moisture rice was significantly inhibited. Additionally, during the high-temperature stress storage period, the total phenol and total flavonoid content in the treatment group increased simultaneously (P<0.05), with maximum increases of 1.23 and 1.34 times, respectively. The antioxidant properties and iron ion reduction capacity of the corresponding samples were also improved (P<0.05). Combined with the analysis of the activity of key limiting enzymes in phenylpropane metabolism, the activities of phenylalanine ammonia-lyase (PAL), cinnamic acid-4-hydroxylase (C4H), and chalcone synthase (CHS) were significantly enhanced, with increases ranging from 1.71 to 2.28 times. Further correlation analysis confirmed the close association (P<0.05) between the content of phenolic substances in high-moisture rice under high-temperature stress and the activities of PAL, C4H, and CHS enzymes, and a significant negative correlation (P<0.05) with internal reactive oxygen species and MDA content. 【Conclusion】 Dielectric barrier discharge cold plasma treatment could promote the synthesis rate of phenolic compounds within the grains of high-moisture rice by activating phenylpropane metabolism. This treatment enhanced the tissue's antioxidant properties, alleviated membrane lipid peroxidation, and reduced the accumulation of free radicals. As a result, it delayed the quality deterioration of rice under high-temperature storage conditions.

Key words: DBD-CP, phenylpropanoid metabolism, phenolic compounds, antioxidant activity, storage

侯帅, 张祎佳, 周丹丹, 马飞洋, 王大鹏, 赵思琪, 丁超, 刘强. 基于介质阻挡放电低温等离子体分析储藏稻谷高温胁迫下酚类代谢规律[J]. 中国农业科学, 2024, 57(6): 1180-1190.

HOU Shuai, ZHANG YiJia, ZHOU DanDan, MA FeiYang, WANG DaPeng, ZHAO SiQi, DING Chao, LIU Qiang. Analysis of the Effect of Dielectric Barrier Discharge Cold Plasma on Phenolic Metabolism of Stored Paddy Rice Under High Temperature Stress[J]. Scientia Agricultura Sinica, 2024, 57(6): 1180-1190.

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基于介质阻挡放电低温等离子体分析储藏稻谷高温胁迫下酚类代谢规律

Analysis of the Effect of Dielectric Barrier Discharge Cold Plasma on Phenolic Metabolism of Stored Paddy Rice Under High Temperature Stress

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