中国农业科学 ›› 2019, Vol. 52 ›› Issue (10): 1807-1817.doi: 10.3864/j.issn.0578-1752.2019.10.013
安清明1,2,周辉通2,3,吴震洋1,罗玉柱2(),JonG.Hickford2,3
收稿日期:
2018-11-05
接受日期:
2019-03-18
出版日期:
2019-05-16
发布日期:
2019-05-23
通讯作者:
罗玉柱
作者简介:
安清明,E-mail: 基金资助:
AN QingMing1,2,ZHOU HuiTong2,3,WU ZhenYang1,LUO YuZhu2(),Jon G. Hickford2,3
Received:
2018-11-05
Accepted:
2019-03-18
Online:
2019-05-16
Published:
2019-05-23
Contact:
YuZhu LUO
摘要:
【目的】检测绵羊ADIPOQ基因多态性及连锁现状,评估该基因突变对绵羊生长及胴体性状的影响,以期丰富绵羊相关重要经济性状的分子遗传研究基础。【方法】以8个不同品种的商品绵羊为研究对象,利用PCR-SSCP方法检测ADIPOQ基因Exon-1区和Exon-2区变异,利用GLMs模型进行评估该基因突变对绵羊生长及胴体性状的影响。【结果】绵羊ADIPOQ基因Exon-1区和Exon-2区共检测到13个突变位点,其中Exon-2区发现的c.46T/C突变导致编码氨基酸p.Tyr16His转变。Exon-1区等位基因A1和B1为优势等位基因,Exon-2区等位基因A2和D2为优势等位基因,且两个区域的等位基因均存在种群差异,大多数品种在两个区域中的变异为中度多态(0.25<PIC<0.5),只有美利奴羊在Exon-2区为高度多态(PIC>0.5),特克塞尔、派伦代和丘陵陶塞特羊在Exon-2区为低度多态(PIC<0.25);两段区域间存在的突变位点为高度连锁,且趋向于共同遗传(D’=0.952,r 2=0.365)。关联分析结果表明,ADIPOQ基因Exon-1区变异对绵羊生长性状存在性别差异,携带等位基因A1的公羔具有较低的断尾重、断奶重和断奶前生长速度(P<0.05),而携带等位基因A1的母羔却与生长性状无显著关联;携带等位基因B1的公羔与生长性状无显著关联,但携带等位基因B1的母羔却具有较高的断尾重(P<0.05);同时发现基因型为B1B1的公羔个体具有更高的断尾重和断奶重(P<0.05);胴体性状关联分析结果表明,携带等位基因A1的群体具有较低的热胴体重、腰部瘦肉量、后腿瘦肉量和总瘦肉量(P<0.05),携带等位基因B1的群体则具有较高的后腿瘦肉量、后腿瘦肉比例和较低的肩部瘦肉比例(P<0.05);基因型为B1B1的个体均有较高的热胴体重、腰部瘦肉量、后腿瘦肉量和总瘦肉量(P<0.05)。 【结论】绵羊ADIPOQ基因的两段区域具有丰富的多态性,Exon-2区域中的c.46T/C为非同义突变。Exon-1区变异影响绵羊的生长性状和胴体性状,淘汰携带等位基因A1的个体和选留存在等位基因B1的个体、或留存B1B1基因型的个体和淘汰A1A1的个体,均可有效改善绵羊后代群体的部分生长性状和胴体性状。
安清明,周辉通,吴震洋,罗玉柱,JonG.Hickford. 绵羊脂联素基因(ADIPOQ)多态性及其与生长及胴体性状关联性分析[J]. 中国农业科学, 2019, 52(10): 1807-1817.
AN QingMing,ZHOU HuiTong,WU ZhenYang,LUO YuZhu,Jon G. Hickford. Polymorphisms of ADIPOQ Gene and Their Association with Growth and Carcass Traits in Sheep[J]. Scientia Agricultura Sinica, 2019, 52(10): 1807-1817.
表3
绵羊ADIPOQ基因Exon-1区和Exon-2区突变位点"
Exon-1 | Exon-2 | ||||||||
---|---|---|---|---|---|---|---|---|---|
突变位点 Mutation sites | 等位基因 Allele | 突变位点 Mutation sites | 等位基因 Allele | ||||||
A1 | B1 | C1 | D1 | A2 | B2 | C2 | D2 | ||
c.-9831 | A | G | A | A | c.46# | A | G | A | A |
c.-9791 | C | C | C | A | c.135 | C | C | C | T |
c.-9790 | G | T | G | G | c.199+24 | G | T | G | G |
c.-9644 | G | G | A | G | c.199+71 | G | G | A | G |
c.-9640 | A | G | A | A | c.199+94 | A | G | A | A |
c.-9632 | C | T | C | C | c.199+126 | C | T | C | C |
c.-9631 | A | G | A | A |
表4
ADIPOQ基因不同区域在各品种中的等位基因频率"
品种 (Breed) | 数量 Number | 外显子1区 Exon-1 | 外显子2 Exon-2 | ||||||
---|---|---|---|---|---|---|---|---|---|
A1 | B1 | C1 | D1 | A2 | B2 | C2 | D2 | ||
美利奴Merino | 68 | 50.00 | 48.53 | 0.00 | 1.47 | 55.15 | 5.15 | 9.55 | 30.15 |
罗姆尼Romney | 71 | 52.82 | 41.55 | 4.93 | 0.70 | 76.76 | 0.00 | 4.93 | 18.31 |
萨福克Suffolk | 42 | 53.57 | 46.43 | 0.00 | 0.00 | 75.00 | 1.19 | 0.00 | 23.81 |
特克塞尔羊Texel | 22 | 56.82 | 43.18 | 0.00 | 0.00 | 100.00 | 0.00 | 0.00 | 0.00 |
考利代羊Corriedale | 41 | 39.02 | 57.32 | 0.00 | 3.66 | 75.61 | 2.44 | 6.10 | 15.85 |
派伦代羊Perendale | 14 | 57.14 | 39.29 | 0.00 | 3.57 | 89.29 | 0.00 | 0.00 | 10.71 |
杜泊羊Dorper | 39 | 76.92 | 23.08 | 0.00 | 0.00 | 56.41 | 1.28 | 38.46 | 3.85 |
丘陵陶塞特Dorset down | 19 | 81.58 | 18.42 | 0.00 | 0.00 | 100.00 | 0.00 | 0.00 | 0.00 |
总数Overall | 316 | 55.70 | 42.09 | 1.11 | 1.10 | 72.78 | 1.74 | 8.70 | 16.78 |
表5
不同绵羊ADIPOQ基因遗传特性及Hardy-Weinberg平衡检验"
品种 Breed | 外显子1 Exon-1 | 外显子2 Exon-2 | 卡方检验域 χ2 testing | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Na | Ne | Ho | He | PIC | Na | Ne | Ho | He | PIC | 外显子1 Exon-1 | 外显子2 Exon-2 | |
美利奴 Merino | 3.00 | 2.06 | 0.53 | 0.47 | 0.40 | 4.00 | 2.45 | 0.32 | 0.68 | 0.53 | P = 0.45 | P<0.01 |
罗姆尼 Romney | 4.00 | 2.02 | 0.61 | 0.39 | 0.45 | 3.00 | 1.60 | 0.59 | 0.41 | 0.33 | P<0.05 | P = 0.53 |
萨福克 Suffolk | 2.00 | 1.99 | 0.40 | 0.60 | 0.37 | 3.00 | 1.61 | 0.52 | 0.48 | 0.32 | P = 0.23 | P = 0.09 |
特克塞尔羊 Texel | 2.00 | 1.96 | 0.68 | 0.31 | 0.37 | 3.00 | 1.58 | 0.57 | 0.43 | 0.00 | P = 0.08 | P = 0.11 |
考利代羊 Corriedale | 3.00 | 2.07 | 0.41 | 0.59 | 0.42 | 4.00 | 1.66 | 0.61 | 0.39 | 0.36 | P = 0.13 | P = 0.12 |
派伦代羊 Perendale | 3.00 | 2.07 | 0.57 | 0.43 | 0.42 | 2.00 | 1.23 | 0.79 | 0.21 | 0.17 | P = 0.67 | P = 0.72 |
杜泊羊 Dorper | 2.00 | 1.55 | 0.64 | 0.36 | 0.29 | 4.00 | 2.14 | 0.46 | 0.54 | 0.44 | P = 0.99 | P<0.01 |
丘陵陶塞特 Dorset Down | 2.00 | 1.43 | 0.63 | 0.37 | 0.26 | 2.00 | 1.72 | 0.67 | 0.33 | 0.00 | P = 0.37 | P = 0.13 |
表6
ADIPOQ基因Exon-1区等位基因与罗姆尼公/母羔羊生长性状关联性分析"
生长性状 Growth traits | 等位 基因 Allele | 其它等位基因 Other Allele in model | 公羔 Male Lambs | 母羔 Female Lambs | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
存在 Present (1) | 数目 Number | 缺失 Absent (0) | 数目 Number | P值 P-value | 存在 Present (1) | 数目 Number | 缺失 Absent (0) | 数目 Number | P值 P-value | |||
初生重 Birth weight (kg) | A1 | None | 5.51±0.16 | 400 | 5.48±0.17 | 150 | 0.800 | 4.90±0.20 | 382 | 4.81±0.21 | 155 | 0.278 |
B1 | None | 5.57±0.16 | 418 | 5.44±0.17 | 132 | 0.173 | 4.88±0.20 | 408 | 4.87±0.22 | 129 | 0.938 | |
断尾重 Tailing weight (kg) | A1 | None | 12.91±0.53 | 400 | 13.61±0.56 | 150 | 0.019 | 10.58±0.62 | 382 | 10.60±0.65 | 155 | 0.934 |
B1 | None | 13.18±0.32 | 418 | 13.62±0.39 | 132 | 0.155 | 10.67±0.61 | 408 | 10.06±0.65 | 129 | 0.019 | |
A1 | B1 | 12.91±0.53 | 400 | 13.75±0.56 | 150 | 0.010 | ||||||
断奶重 Weaning weight (kg) | A1 | None | 30.43±0.90 | 400 | 31.64±0.95 | 150 | 0.017 | 25.05±1.05 | 382 | 24.86±1.10 | 155 | 0.661 |
B1 | None | 30.04±0.55 | 418 | 30.75±0.66 | 132 | 0.179 | 25.12±1.04 | 408 | 24.38±1.10 | 129 | 0.089 | |
A1 | B1 | 30.52±0.91 | 400 | 31.85±0.96 | 150 | 0.010 | ||||||
断奶前生长速度 Pre-weaning growth rate (g·d-1) | A1 | None | 289.10±9.3 | 400 | 300.80±9.7 | 150 | 0.025 | 234.20±1.1 | 382 | 233.60±1.2 | 155 | 0.887 |
B1 | None | 279.60±5.7 | 418 | 284.80±6.9 | 132 | 0.355 | 234.90±1.1 | 408 | 229.40±1.1 | 129 | 0.233 |
表7
ADIPOQ基因Exon-1区基因型与罗姆尼公/母羔羊生长性状关联性分析"
生长性状 Growth traits | 基因型 Genotype | 公羔 Male lambs | 母羔 Female lambs | ||||
---|---|---|---|---|---|---|---|
平均值±标准误 Mean±SE | 数目 Number | P值 P-value | 平均值±标准误 Mean±SE | 数目 Number | P值 P-value | ||
初生重 Birth weight (kg) | A1A1 | 5.42±0.17 | 132 | 0.291 | 4.87±0.22 | 129 | 0.521 |
A1B1 | 5.56±0.17 | 268 | 4.90±0.21 | 253 | |||
B1B1 | 5.51±0.17 | 150 | 4.81±0.21 | 155 | |||
断尾重 Tailing weight (kg) | A1A1 | 13.17±0.56 | 132 | 0.026 | 10.06±0.65 | 129 | 0.056 |
A1B1 | 12.76±0.54 | 268 | 10.70±0.62 | 253 | |||
B1B1 | 13.54±0.56 | 150 | 10.57±0.64 | 155 | |||
断奶重 Weaning weight (kg) | A1A1 | 30.82±0.96 | 132 | 0.028 | 24.38±1.10 | 129 | 0.160 |
A1B1 | 30.20±0.92 | 268 | 25.22±1.05 | 253 | |||
B1B1 | 31.54±0.95 | 150 | 24.83±1.09 | 155 | |||
断奶前生长速度 Pre-weaning growth rate(g·d-1) | A1A1 | 291.90±9.9 | 132 | 0.055 | 229.40±11.7 | 129 | 0.445 |
A1B1 | 287.30±9.5 | 268 | 235.40±11.1 | 253 | |||
B1B1 | 300.00±9.8 | 150 | 233.30±11.6 | 155 |
表8
ADIPOQ基因启动子变异体对罗姆尼羔羊胴体肌肉性状的影响"
性状 Traits | 等位基因 Allele | 其它等位基因 Other allele in model | 平均值±标准误 (Mean ± SE) | P值 P-value | |||
---|---|---|---|---|---|---|---|
存在 Present | 数量 Number | 缺失 Absent | 数量 Number | ||||
热胴体重(kg) Hot weight (H-W) | A1 | None | 17.04 ± 0.29 | 331 | 17.34 ± 0.31 | 125 | 0.086 |
B1 | None | 17.07 ± 0.29 | 341 | 17.32 ± 0.32 | 115 | 0.149 | |
A1 | B1 | 17.07 ± 0.30 | 331 | 17.45 ± 0.32 | 125 | 0.036 | |
B1 | A1 | 17.09 ± 0.30 | 341 | 17.43 ± 0.32 | 115 | 0.060 | |
GR值(cm) GR value (V-GR) | A1 | None | 2.36 ± 0.48 | 331 | 2.19 ± 0.49 | 125 | 0.550 |
B1 | None | 2.17 ± 0.47 | 341 | 2.54 ± 0.50 | 115 | 0.196 | |
A1 | B1 | 2.39 ± 0.48 | 331 | 2.30 ± 0.51 | 125 | 0.752 | |
B1 | A1 | 2.17 ± 0.18 | 341 | 2.52 ± 0.51 | 115 | 0.234 | |
后退瘦肉量(%) Leg yield | A1 | None | 20.69 ± 0.21 | 331 | 21.09 ± 0.21 | 125 | 0.001 |
B1 | None | 20.93 ± 0.21 | 341 | 20.67 ± 0.22 | 115 | 0.039 | |
A1 | B1 | 20.68 ± 0.21 | 331 | 21.04 ± 0.22 | 125 | 0.004 | |
B1 | A1 | 20.95 ± 0.20 | 341 | 20.77 ± 0.22 | 115 | 0.164 | |
腰部瘦肉量(%) Loin yield | A1 | None | 14.16 ± 0.15 | 331 | 14.41 ± 0.16 | 125 | 0.006 |
B1 | None | 14.28 ± 0.15 | 341 | 14.20 ± 0.16 | 115 | 0.359 | |
A1 | B1 | 14.16 ± 0.15 | 331 | 14.40 ± 0.16 | 125 | 0.009 | |
B1 | A1 | 14.29 ± 0.15 | 341 | 14.27 ± 0.16 | 115 | 0.755 | |
肩部瘦肉量(%) Shoulder yield | A1 | None | 16.93 ± 0.17 | 331 | 17.12 ± 0.18 | 125 | 0.067 |
B1 | None | 16.99 ± 0.17 | 341 | 17.05 ± 0.18 | 115 | 0.550 | |
A1 | B1 | 16.95 ± 0.17 | 331 | 17.16 ± 0.18 | 125 | 0.044 | |
B1 | A1 | 16.70 ± 0.17 | 341 | 17.11 ± 0.18 | 115 | 0.301 | |
总瘦肉量(%) Total yield | A1 | None | 51.79 ± 0.43 | 331 | 52.63 ± 0.45 | 125 | 0.001 |
B1 | None | 52.20 ± 0.43 | 341 | 51.92 ± 0.45 | 115 | 0.281 | |
A1 | B1 | 51.79 ± 0.43 | 331 | 52.60 ± 0.45 | 125 | 0.002 | |
B1 | A1 | 52.24 ± 0.43 | 341 | 52.14 ± 0.46 | 115 | 0.712 | |
后腿瘦肉比例(%) Proportion of leg yield | A1 | None | 39.94 ± 0.21 | 331 | 40.07 ± 0.22 | 125 | 0.290 |
B1 | None | 40.09 ± 0.20 | 341 | 39.79 ± 0.22 | 115 | 0.017 | |
A1 | B1 | 39.92 ± 0.21 | 331 | 39.98 ± 0.22 | 125 | 0.589 | |
B1 | A1 | 40.09 ± 0.21 | 341 | 39.81 ± 0.22 | 115 | 0.027 | |
腰部瘦肉比例(%) Proportion of loin yield | A1 | None | 27.34 ± 0.17 | 331 | 27.37 ± 0.18 | 125 | 0.733 |
B1 | None | 27.35 ± 0.17 | 341 | 27.35 ± 0.18 | 115 | 0.932 | |
A1 | B1 | 27.34 ± 0.17 | 331 | 27.37 ± 0.18 | 125 | 0.742 | |
B1 | A1 | 27.36 ± 0.17 | 341 | 27.36 ± 0.18 | 115 | 0.992 | |
肩部瘦肉比例(%) Proportion of shoulder yield | A1 | None | 32.72 ± 0.22 | 331 | 32.55 ± 0.23 | 125 | 0.209 |
B1 | None | 32.55 ± 0.22 | 341 | 32.86 ± 0.23 | 115 | 0.020 | |
A1 | B1 | 32.74 ± 0.22 | 331 | 32.64 ± 0.23 | 125 | 0.447 | |
B1 | A1 | 32.55 ± 0.22 | 341 | 32.83 ± 0.23 | 115 | 0.036 |
表9
ADIPOQ基因启动子基因型对罗姆尼羔羊胴体肌肉性状的影响"
性状 Traits | 基因型 Genotype | 数量 Number | 平均值±标准误 Mean ± SE | P值 P-value |
---|---|---|---|---|
热胴体重(kg) Hot weight (H-W) | A1A1 | 115 | 17.24 ± 0.32 | 0.039 |
A1B1 | 216 | 16.89 ± 0.31 | ||
B1B1 | 125 | 17.27 ± 0.31 | ||
GR值(cm) GR value (V-GR) | A1A1 | 115 | 2.56 ± 0.51 | 0.412 |
A1B1 | 216 | 2.21 ± 0.49 | ||
B1B1 | 125 | 2.12 ± 0.50 | ||
后腿瘦肉量(%) Leg yield | A1A1 | 115 | 20.59 ± 0.22 | 0.002 |
A1B1 | 216 | 20.77 ± 0.21 | ||
B1B1 | 125 | 21.13 ± 0.21 | ||
腰部瘦肉量(%) Loin yield | A1A1 | 115 | 14.14 ± 0.16 | 0.021 |
A1B1 | 216 | 14.17 ± 0.15 | ||
B1B1 | 125 | 14.41 ± 0.16 | ||
肩部瘦肉量(%) Shoulder yield | A1A1 | 115 | 17.00 ± 0.18 | 0.109 |
A1B1 | 216 | 16.89 ± 0.18 | ||
B1B1 | 125 | 17.10 ± 0.18 | ||
总瘦肉量(%) Total yield | A1A1 | 115 | 51.74 ± 0.45 | 0.005 |
A1B1 | 216 | 51.84 ± 0.44 | ||
B1B1 | 125 | 52.65 ± 0.45 | ||
后腿瘦肉比例(%) Proportion of leg yield | A1A1 | 115 | 39.78 ± 0.22 | 0.050 |
A1B1 | 216 | 40.06 ± 0.21 | ||
B1B1 | 125 | 40.13 ± 0.22 | ||
腰部瘦肉比例(%) Proportion of loin yield) | A1A1 | 115 | 27.34 ± 0.18 | 0.944 |
A1B1 | 216 | 27.34 ± 0.17 | ||
B1B1 | 125 | 27.37 ± 0.18 | ||
肩部瘦肉比例(%) (Proportion of shoulder yield | A1A1 | 115 | 32.88 ± 0.23 | 0.050 |
A1B1 | 216 | 32.60 ± 0.23 | ||
B1B1 | 125 | 32.50 ± 0.23 |
[1] | YANG Y, ZHANG F, DING R, SKRIP L, WANG Y, LEI H, HU D . ADIPOQ gene polymorphisms and cancer risk: a meta-analysis. Cytokine, 2013,61(2):565-671. |
[2] |
YANG W S, CHUANG L M . Human genetics of adiponectin in the metabolic syndrome. Journal of Molecular Medicine, 2006,84(2):112-121.
doi: 10.1007/s00109-005-0011-7 |
[3] |
SCHERER P E, WILLIAMS S, FOGLIANO M, BALDINI G, LODISH H F . A Novel Serum Protein Similar to C1q, Produced Exclusively in Adipocytes. Journal of Biological Chemistry, 1995,270(45):26746-26749.
doi: 10.1074/jbc.270.45.26746 |
[4] |
HSUEH W C, PL S J, MITCHELL B D, POLLIN T I, KNOWLER W C, EHM M G, BELL C J, SAKUL H, WAGNER M J, BURNS D K . Genome-wide and fine-mapping linkage studies of type 2 diabetes and glucose traits in the Old Order Amish: evidence for a new diabetes locus on chromosome 14q11 and confirmation of a locus on chromosome 1q21-q24. Diabetes, 2003,52(2):550-557.
doi: 10.2337/diabetes.52.2.550 |
[5] |
YAMAUCHI T, KAMON J, ITO Y, TSUCHIDA A, YOKOMIZO T, KITA S, SUGIYAMA T, MIYAGISHI M, HARA K, TSUNODA M . Cloning of adiponectin receptors that mediate antidiabetic metabolic effects. Nature, 2003,423(6941):762-769.
doi: 10.1038/nature01705 |
[6] | KHARROUBI I, RASSCHAERT JEIZIRIK D L, CNOP M . Expression of adiponectin receptors in pancreatic beta cells. Biochemical & Biophysical Research Communications, 2003,312(4):1118-1122. |
[7] | YAMAUCHI T, HARA K, KUBOTA N, TERAUCHI Y, TOBE K, FROGUEL P, NAGAI R, KADOWAKI T . Dual roles of adiponectin/Acrp30 in vivo as an anti-diabetic and anti-atherogenic adipokine. Current Drug Targets - Immune Endocrine & Metabolic Disorders, 2003,3(3):243-253. |
[8] | 孟宪然, 杜琛, 王静, 付绍印, 郑竹清, 张文广, 李金泉 . 基于RNA-Seq识别山羊肉品质候选基因. 畜牧兽医学报, 2015,46(08):1300-1307. |
MENG X R, DU C, WANG J, FU S Y, ZHENG Z Q, ZHANG W G, LI J Q . RNA-Seq Approach for Identifying Candidate Genes of Meat Quality in Goats. Acta Veterinariaet Zootechnica Sinica, 2015,46(08):1300-1307. (in Chinese) | |
[9] | CIESLAK J, FLISIKOWSKA T, SCHNIEKE A, KIND A, SZYDLOWSKI M, SWITONSKI M, FLISIKOWSKI K . Polymorphisms in the promoter region of the adiponectin (ADIPOQ) gene are presumably associated with transcription level and carcass traits in pigs. Animal Genetics, 2013,44(3):340-343. |
[10] |
DAI L H, XIONG Y Z, DENG C Y, JIANG S W, ZUO B, ZHENG R, LI F E, LEI M . Association of the A-G polymorphism in porcine adiponectin gene with fat deposition and carcass traits. Asian Australasian Journal of Animal Sciences, 2006,19(6):779-783.
doi: 10.5713/ajas.2006.779 |
[11] |
MORSCI N S, SCHNABEL R D, TAYLOR J F . Association analysis of adiponectin and somatostatin polymorphisms on BTA1 with growth and carcass traits in Angus cattle. Animal Genetics, 2006,37(6):554-562.
doi: 10.1111/age.2006.37.issue-6 |
[12] | SHIN S, CHUNG E . Novel SNPs in the bovine ADIPOQ and PPARGC1A genes are associated with carcass traits in Hanwoo (Korean cattle). Molecular Biology Reports, 2013,40(7):4651-4660. |
[13] | 刘重旭, 王凭青, 张宝云, 储明星, 邓腊梅, 谭颖, 樊奇 . 贵州白山羊和古蔺马羊脂联素基因多态性及其与繁殖性能的关联研究. 中国农业科学, 2011,44(9):1916-1922. |
LIU C X, WANG P Q, ZHANG B Y, CHU M X, DENG L M, TAN Y, FAN Q . Polymorphism of adiponectin gene and its relationship with reproductive ability in Guizhou White and Gulin Ma Goats. Scientia Agricultura Sinica, 2011,44(9):1916-1922. (in Chinese) | |
[14] |
ZHOU H, HICKFORD J.G. H. , FANG Q . A two-step procedure for extracting genomic DNA from dried blood spots on filter paper for polymerase chain reaction amplification. Analytical Biochemistry, 2006,354(1):159-161.
doi: 10.1016/j.ab.2006.03.042 |
[15] |
SLATKIN M . Linkage disequilibrium--understanding the evolutionary past and mapping the medical future. Nature Reviews Genetics, 2008,9(6):477-485.
doi: 10.1038/nrg2361 |
[16] |
ARDLIE K G, KRUGLYAK L, SEIELSTAD M . Patterns of linkage disequilibrium in the human genome. Nature Reviews Genetics, 2002,3(4):299-309.
doi: 10.1038/nrg777 |
[17] | CHU H, WANG M, ZHONG D, SHI D, MA L, TONG N, ZHANG Z . ADIPOQ polymorphisms are associated with type 2 diabetes mellitus: A meta‐analysis study. Diabetes/metabolism research and reviews, 2013,29(7):532-545. |
[18] | YUAN Y, JIANG H, KUANG J, HOU X, FENG Y, SU Z . Genetic variations in ADIPOQ gene are associated with chronic obstructive pulmonary disease. PloS One, 2012,7(11):e50848. |
[19] | HOUDE A, MURPHY B, MATHIEU O, BORDIGNON V, PALIN M F . Characterization of swine adiponectin and adiponectin receptor polymorphisms and their association with reproductive traits. Animal Genetics, 2010,39(3):249-257. |
[20] |
FANG X, DU Y, ZHANG C, SHI X Y, CHEN D X, SUN J J, JIN Q J, LAN X Y, CHENG H . Polymorphism in a microsatellite of the acrp30 gene and its association with growth traits in goats. Biochemical Genetics, 2011,49(7-8):533-539.
doi: 10.1007/s10528-011-9428-6 |
[21] |
YANG H, YE E, SI G, CHENG L M, CAI L Q, YE C F, ZHANG C, LU X M . Adiponectin gene polymorphism rs2241766 T/G is associated with response to pioglitazone treatment in type 2 diabetic patients from Southern China. PloS One, 2014,9(11):e112480
doi: 10.1371/journal.pone.0112480 |
[22] | GU H F . Biomarkers of adiponectin: plasma protein variation and genomic DNA polymorphisms. Biomarker insights, 2009,4(4):123-133. |
[23] | 沈留红, 江涛, 巫晓峰, 姜思汛, 肖劲邦, 曹随忠, 余树民, 邓俊良, 左之才, 彭广能, 马晓平, 钟志军, 任志华, 王娅, 胡延春 . 奶牛胎盘脂联素、瘦素、内脂素与犊牛初生重相关性研究. 畜牧兽医学报, 2017,48(01):185-192. |
SHEN L H, JIANG T, WU X F, JIANG S X, XIAO J B, CAO S Z, YU S M, DENG J L, ZUO Z C, PENG G N, MA X P, ZHONG Z J, REN Z H, WANG Y, HU Y C . The Correlation between adiponectin, leptin, visfatin in placenta and calf birth weight. Acta Veterinariaet Zootechnica Sinica, 2017,48(01):185-192. (in Chinese) | |
[24] | RIESTRA P, GEBREAB S Y, XU R, KHAN R J, BIDULESCU A, CORREA A, TEKOLA-AYELE F, DAVIS S K . Gender-specific associations between ADIPOQ gene polymorphisms and adiponectin levels and obesity in the Jackson Heart Study cohort. BMC Medical Genetics, 2015,16(1):65. |
[25] | REISZPORSZASZ S, BHASIN S, ARTAZA J N, SHEN R, SINHA- HIKIM I, HOUGUE A, FIELDER T J, GONZALEA- CADAVID N F . Lower skeletal muscle mass in male transgenic mice with muscle- specific overexpression of myostatin. Ajp Endocrinology & Metabolism, 2003,285(4):E876-888. |
[26] |
HAN J, ZHOU H, FORREST R H, SEDCOLE J R, FRAMPTON C M, HICKFORD J G H . Effect of myostatin (MSTN) g+6223G > A on production and carcass traits in New Zealand Romney Sheep. Asian-Australasian Journal of Animal Sciences, 2010,23(7):863-866.
doi: 10.5713/ajas.2010.90392 |
[27] |
LEE Y S, LEE S J . Regulation of GDF-11 and myostatin activity by GASP-1 and GASP-2. PNAS, 2013,110(39):E3713-3722.
doi: 10.1073/pnas.1309907110 |
[28] |
WANG J Q, ZHOU H, FANG Q, LIU X, LUO Y Z, HICKFORD J G H . Effect of variation in ovine WFIKKN2 on growth traits appears to be gender-dependent. Scientific Report, 2015,5:12347; doi: 10.1038/srep12347.
doi: 10.1038/srep12347 |
[29] | VOLK M G . An examination of the evidence supporting the association of dietary cholesterol and saturated fats with serum cholesterol and development of coronary heart disease. Alternative Medicine Review, 2007,12(3):228-246. |
[30] | OWECKI M, MICZKE A, KACZMAREK M, HOPPE-GOLEBIEWSKA J, PUPEK-MUSIALIK D, SLOMSKI R, BRYLL W, CYMERYS M, NIKISCH E, SOWIŃSKI J . The Y111 H (T415C) polymorphism in exon 3 of the gene encoding adiponectin is uncommon in Polish obese patients. Hormone & Metabolic Research, 2007,39(11):797-800. |
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