关键词: 黄瓜, 野生酸黄瓜, 渐渗系, DNA序列, 农艺性状

Abstract:

【Objective】 Introgression lines were researched by molecular markers and the phenotypic identification in order to proof DNA flow from wild sour cucumber to cultivated cucumber, which were obtained by interspecific hybridization between the cultivated cucumber (Cucumis sativus L., 2n=14) and wild sour cucumber (Cucumis hystrix Chakr., 2n=24), and multiple generational backcrossing and selfing followed. 【Method】 Molecular markers, DNA sequences and agronomic traits were used in the study to analyze and contrast introgression lines and their parents. 【Result】 The results showed that the genetic polymorphism between introgression lines and cultivated cucumber was 13.2%, including donor specific bands, missing bands and new bands. The sour cucumber specific bands in introgression lines were obtained by RAPD primer D-11 and SSR primer 06632, with the size of 310 bp and 150 bp respectively. Sequence analysis showed that the identity was 92.93% and 96.48%, respectively, though there were 41 and 9 different base pairs, respectively, including the base pairs transition, transversion and deletion. And the genetic characteristics of sour cucumber were found in introgression lines. 【Conclusion】 These results confirmed that a few mutations of different types occurred with DNA segments of wild sour cucumber being introgressed into cultivated cucumber.

Key words: Cucumis sativus L., wild sour cucumber, introgression lines, DNA sequence, agronomic traits