关键词: 阿维菌素, 甲氨基阿维菌素苯甲酸盐, 伊维菌素, 残留, 高效液相色谱

Abstract:

【Objective】 The aim of the study is to build a sensitive high-performance liquid chromatography with fluorescence detection (HPLC-FD) method for determinating multi-residues of abamectin(ABA), emamectin benzoate (EMA) and ivermectin (IVM) in vegetables. 【Method】 After being extracted by acetonitrile, the avermectins residues in vegetables were directly derivatised by N-methylimidazole (N-NMIM) and trifluoroacetic anhydride (TFAA) and then analyzed on HPLC-FLD using gradient elution program with acetonitrile/methanol/water. 【Result】 Results showed that the developed method had good sensitivity and selectivity for determining the ABA, EMA and IVM residues in vegetables within 12 min. When spiked at 10-1 000 μg?L-1, the fortified recoveries of ABA, EMA and IVM, respectively, were 90.5%-114.1%, 96.7%-118%, and 92.8%-108.1%, and their variation coefficients (C.V. %) were 0.85%-12.11%, 1.38%-9.36%, and 0.95%-11.52%, respectively. The limited detection levels of ABA, EMA and IVM residues in vegetables were 1.2, 2.1, and 1.7 μg?kg-1, respectively. 【Conclusion】 The HPLC-FD method is simple, fast and easy to perform, all validated parameters of this method are sufficient for analysis of pesticide residue.

Key words: abamectin, emamectin benzoate, ivermectin, residues, HPLC