关键词: 大白菜, 结球甘蓝, 核型分析, 分子标记, 二体异附加系

Abstract:

【Objective】 The objective of this study is to obtain homozygous Chinese cabbage-cabbage disomic alien addition lines. 【Method】 In this study, 5#Chinese cabbage-cabbage monosomic addition line was used, and its selfing F1 generation was tested by means of identification of cytology and SSR analysis. 【Result】 The results showed that the ratio of disomic addition lines (2n=22) was 10.81% in 37 screened plants of selfed progeny. Through karyotype analysis and molecular marker identification, 4 Chinese cabbage-cabbage disomic alien addition lines (Chinese cabbage with 5# chromosome from cabbage) were identified in the plants with 2n=22. The alien chromosomes of disomic alien addition lines moving late was observed at anaphaseⅠof meiotic behavior, but the distribution of most of the chromosomes was 11-11-11-11 at anaphaseⅡ, the ratio was 69.55%. 【Conclusion】 5# Chinese cabbage-cabbage disomic alien addition lines were obtained. The alien chromosome of 5# Chinese cabbage-cabbage disomic alien addition lines corresponded with 07 linkage group of cabbage. Disomic alien addition lines undergo meiosis to form the tetrad which is mainly additional one chromosome of the cabbage (n=11), it has higher hereditary stability. The acquisition of alien addition lines for the study of the phylogenetic relationships between brassica A and C genome, and for transmitting valuable genes from cabbage to Chinese cabbage and expanding the genetic background of Chinese cabbage germplasm, are of great significance.

Key words: Chinese cabbage, cabbage, karyotype analysis, molecular marker, disomic alien addition lines