关键词: 花生, 遗传多样性, 分子标记, 聚类分析

Abstract:

【Objective】 In order to provide theoretical information for peanut breeding,an experiment was carried out to study the genetic relationship and genetic diversity of peanut germplasms. 【Method】 32 ISSR and 252 SRAP markers were used to amplify the genomic DNA isolated from 24 peanut genotypes, detecting the inter-specific genetic variation. 【Result】 The result showed that three hundred and ninety DNA fragments were amplified, by using thirteen primers screened out of 32 ISSR primers, among which 293 DNA bands were polymorphic and 75.13 percent of the bands can reveal the genetic diversity between these germplasms. There were 5 827 DNA fragments were the visual bands using 252 SRAP markers, and the polymorphic bands were 3 966 with an average of 17.32 alleles per primer. The genetic similarity (GS) indexes among the 24 peanut genotypes were calculated based on the data from these markers. The value of GS varied from 0.60 to 0.80. The results of UPGMA indicated that the majority of 24 accessions could be divided into seven or eight groups, and most genotypes were clustered by market types. 【Conclusion】 It is no doubt that ISSR and SRAP markers are very valuable to analyze DNA polymorphism and genetic relationship in peanut germplasm.

Key words: peanut, genetic diversity, molecular markers, cluster analysis