关键词: 大葱, 洋葱, 子房培养, 种间杂种, 鉴定, 特性

Abstract:

【Objective】 The objective of the study is to develop the interspecific hybrid plants between welsh onions and onion. 【Method】 The Zhangqiu welsh onion and onion inbred line 06-38 were used to make distant hybridization. The ovary of distant hybrids were cultured. The hybrid plants were identified by CAPS. The fertility was identified by pollen staining, in vitro germination and self-pollination. 【Result】 Under the natural conditions, the embryo of the welsh onion×onion reciprocal cross plants was abortive. The seventh day after pollination was the best time for the ovary culture. The ovarys were cultured on the MS medium without hormone and the welsh onion×onion reciprocal crosses were obtained from the interspecific F1 hybrid. Through ovary culture, 26 hybrid plants of the welsh onion × onion and 22 hybrid plants of onion × onion were obtained. All of the tested hybrids were the true hybrid by CAPS. The F1 hybrid plants had obvious heterosis and hybrid male sterility. 【Conclusion】 By using the ovary culture technique, the interspecific hybrid F1was obtained, thus overcoming the welsh onion×onion distant hybrid which is abortive under natural conditions. The hybrid plants showed apparent heteroses and male sterility.

Key words: welsh onion, onion, ovary culture, interspecific hybrids, identification, characterization