中国农业科学 ›› 2006, Vol. 39 ›› Issue (12): 2406-2415 .

• 作物遗传育种·种质资源 • 上一篇    下一篇

小麦优良种质资源高分子量麦谷蛋白亚基组成分析

张玲丽,李秀全,杨欣明,李洪杰,王 辉,李立会   

  1. 中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程
  • 收稿日期:2006-01-04 修回日期:2006-02-28 出版日期:2006-12-10 发布日期:2006-12-10
  • 通讯作者: 李立会

High-molecular-weight Glutenin Subunit Compositions of Chinese Common Wheat (Triticum aestivum L.) Germplasms

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  1. 中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程
  • Received:2006-01-04 Revised:2006-02-28 Online:2006-12-10 Published:2006-12-10

摘要: 【目的】分析近期育成的、具有一个或多个突出农艺性状的186份优良小麦种质资源的高分子量谷蛋白亚基(HMW-GS)组成和1B/1R异位分布情况,为广泛有效地利用这些新种质提供有用信息。【方法】采用SDS-PAGE方法对优良种质资源的HMW-GS组成进行了分析,并对麦谷蛋白亚基进行品质评分;同时用黑麦1RS上特有的醇溶蛋白标记对上述材料进行1B/1R易位检测。【结果】供试材料在编码HMW-GS位点上具有较大的变异,共发现16个等位变异,形成30种HMW-GS组合形式。在Glu-1的3个位点具有2个及2个以上优质亚基的材料占38.7%(72份),其中烘拷品质评分为10分且具有高产、矮秆、抗病、抗逆等一个或多个突出农艺性状的新种质13份。携带有1B/1R易位的材料为68份,占供试材料的36.6%。【结论】这些结合有优异性状的优质材料将是中国小麦遗传育种的宝贵优质源。

关键词: 小麦, 高分子量谷蛋白亚基(HMW-GS), 1B/1R易位

Abstract: Variation in the electrophoretic banding patterns of high-molecular-weight glutenin subunits (HMW-GS) of 186 common wheat (Triticum aestivum L.) germplasms that originated from China was examined by sodium dodecyl sulfate polyacrylamide gel electrophoresis in order to improve the effective utilization of these germplasms. The bread-making quality score of each genotype was calculated. The high level of genetic variation at Glu-1 locus was observed. Seventeen major glutenin HMW-GS were detected and 30 HMW-GS compositions were observed. The percentage of cultivars with more than two subunits related to good quality were 38.7% among 186 wheat lines. There were 13 lines with bread-making quality scores of 10 in combination with one or two good agronomical traits, such as high-yield, dwarfing, resistance to diseases and/or tolerant abiotic stress. These new germplasms can be the important resources for improvment bread making quality.

Key words: Wheat germplasm, High-molecular-weight glutenin subunits (HMW-GS), Quality scores, germplasms