关键词: 开口箭, 提取物, 荔枝霜疫霉菌, 抗菌活性, 贮藏

Abstract: 【Objective】To exploit plant-derived antifungal substances as an alternative strategy to control post-harvest diseases of fruits and vegetables.【Method】The MeOH extract of the rhizomes of Tupistra chinensis was fractioned sequentially with petroleum ether, EtOAc and n-BuOH. The antifungal activities of the MeOH extract and its fractions against Peronophythora litchii were evaluated both in vitro and in vivo using agar plate tests and intact fruit inoculated with Peronophythora litchii. Storage experiments of litchi fruits treated with the MeOH extract and its fractions were carried out.【Result】The EC50 values of the MeOH extract, its EtOAc fraction and n-BuOH fraction inhibiting the mycelia growth of P. litchii were 1598.10, 662.86 and 1147.31 ?g?ml-1, respectively. Dipping litchi fruit in the MeOH extract, its EtOAc fraction or n-BuOH fraction solution resulted in a significant reduction of P. litchi inoculation infection. Additionally, the use of the above extract or fractions was significantly effective in reducing natural incidence of diseases and retarding quality deterioration of litchi fruits during storage.【Conclusion】The MeOH extract of T.chinensis, its EtOAc fraction and n-BuOH fraction were distinct active against Peronophythora litchii both in vitro and in vivo. Treatment of litchi fruits with the extract or fractions had beneficial storage effects.

Key words: Tupistra chinensis Bak., Extract, Peronophythora litchii, Antifungal activity, Storage