中国农业科学 ›› 2004, Vol. 37 ›› Issue (10): 1560-1563 .

• 畜牧.兽医.资源昆虫 • 上一篇    下一篇

能量条件对鸭下丘脑5-羟色胺样神经元的影响

刘华珍,彭克美   

  1. 华中农业大学动物科技-动物医学学院
  • 收稿日期:2003-10-15 修回日期:2004-07-14 出版日期:2004-10-20 发布日期:2004-10-20
  • 通讯作者: 刘华珍

Effect of Energy on Serotonin-like Neurons in Duck Hypothalamus

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  1. 华中农业大学动物科技-动物医学学院
  • Received:2003-10-15 Revised:2004-07-14 Online:2004-10-20 Published:2004-10-20

摘要: 选用来自同一父母代的1日龄健康樱桃谷鸭60羽,饲喂1周后,随机分为2组:高能组和低能组,分别饲喂高能和低能日粮。饲喂4周后,处理动物:灌流固定,取全脑后固定4 h,入8%蔗糖溶液中4℃保存。制作石蜡切片,采用免疫组化链霉亲和素-生物素-过氧化物酶复合物法( Streptavidin -biotin-peroxidase complex method,SABC)进行染色,并用图像分析系统作半定量分析。结果显示:高能组室旁核(17.73±1.41个/19.46×103μm2)及腹内侧核(15.80±1.71个/ 19.46×103μm2)有大量的阳性胞体;低能组外侧区(11.25±1.53个/19.46×103μm2)也有大量的阳性胞体,而室旁核没有。上述研究结果表明,下丘脑5-羟色胺样神经元参与摄食和能量代谢的调节。

关键词: 樱桃谷鸭, 5 -羟色胺, 下丘脑, 摄食, 能量代谢

Abstract: In the present study, the immunohistochemical method streptavidin-biotin-peroxidase complex (SABC) was used to examine the effect of energy on serotonin-like neurons in hypothalamus of Cherry valley ducks bred with high energy feed and low energy feed, respectively. All films were analysed by using a computer-assisted image analysis system. In high energy group, labeled cells are widely distributed in paraventricular nucleus(PVN,17.73±1.41 neurons/19.46×103μm2) and ventromedial hypothalamus (VMH, 15.80±1.71 neurons/19.46×103μm2). In low energy group, labeled cells are widely distributed in lateral hypothalamus(LH,11.25±1.53 neurons/ 19.46×103μm2), yet none in PVN.These results indicate that serotonin-like neurons in hypothalamus are involved in the regulation of food intake and energy metabolism.

Key words: Cherry valley duck, Serotonin, Hypothalamus, Food intake, Energy metabolism