西藏拉鲁湿地野生植物根际微生物资源挖掘及其在缓解玉米盐碱胁迫中的作用

doi:10.3864/j.issn.0578-1752.2025.20.009

中国农业科学 ›› 2025, Vol. 58 ›› Issue (20): 4144-4157.doi: 10.3864/j.issn.0578-1752.2025.20.009

西藏拉鲁湿地野生植物根际微生物资源挖掘及其在缓解玉米盐碱胁迫中的作用

杨芾¹^,²(), 韦善君³(), 张晓霞¹^,²(), 张琳敏³, 刘洪秀³, 李怡君¹^,²^,³, 仝亚萍³

¹ 北方干旱半干旱耕地高效利用全国重点实验室（中国农业科学院农业资源与农业区划研究所），北京 100081
² 国家盐碱地综合利用技术创新中心，山东东营 257347
³ 中央民族大学生命与环境科学学院，北京 100081

收稿日期:2025-07-15 接受日期:2025-09-29 出版日期:2025-10-16 发布日期:2025-10-14
通信作者:
韦善君，E-mail：wei.s.j@163.com。

张晓霞，E-mail：zhangxiaoxia@caas.cn。
联系方式: 杨芾，E-mail：yangfu0326@caf.ac.cn。
基金资助:
中国农业科学院科技创新工程(CAAS-ZDRW202407)

Characterization and Utilization of Rhizosphere Microbiota from Wild Plants in Lalu Wetland in Xizang for Alleviating Saline-Alkali Stress in Maize

YANG Fu¹^,²(), WEI ShanJun³(), ZHANG XiaoXia¹^,²(), ZHANG LinMin³, LIU HongXiu³, LI YiJun¹^,²^,³, TONG YaPing³

¹ State Key Laboratory of Efficient Utilization of Arid and Semi-Arid Arable Land in Northern China (Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences), Beijing 100081
² National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Dongying 257347, Shandong
³ College of Life and Environmental Science, Minzu University of China, Beijing 100081

Received:2025-07-15 Accepted:2025-09-29 Published:2025-10-16 Online:2025-10-14

摘要/Abstract

摘要：

【目的】盐碱化已经成为世界农业发展的重要因素制约之一，微生物可缓解盐碱对植物的胁迫，但在长期耕作过程中可能使土地中有益的微生物类群数量下降或缺失。将源自原始生境野生植物根际的益生菌应用于盐碱地，为作物抗盐碱提供一种新型生物策略，以期减缓盐碱逆境对作物的胁迫。【方法】分离培养西藏拉鲁湿地国家自然保护区5种湿地植物根际细菌，基于16S rRNA基因序列进行初步鉴定；依托鉴别培养基平板进行功能评价，对具有应用前景的新类群通过盆栽试验验证对玉米幼苗生长的影响。【结果】从小蓬草、牛膝菊、曼陀罗、蒲公英和黄芪的根际土壤样品中，共分离得到260株细菌，优势菌属是不动杆菌属（Acinetobacter)，包含发现14个种21株潜在新种；筛选获得溶解无机磷功能34株，产铁载体功能47株。通过基因组确定具产铁载体功能菌株3-210和3-218为黄杆菌属（Flavobacterium）的2个潜在新种，均预测出1个植物激素信号转导关键基因GS2，Fegenie分别预测出了50个和71个与铁相关的基因，未检测到与致病过程相关的毒力因子基因。盆栽试验结果表明，接种2株黄杆菌可提高玉米幼苗株高和茎粗等农艺性状，3-210的处理显著提升了叶片过氧化氢酶活性和游离脯氨酸含量，3-218的处理显著提升了叶片过氧化氢酶和过氧化物酶活性。【结论】西藏拉鲁湿地植物根际土功能细菌资源丰富，筛选获得了多株具有产铁载体和溶磷功能的菌株，获得的黄杆菌属2个潜在新种有增强玉米幼苗适应盐碱胁迫的能力。

关键词: 拉鲁湿地国家级自然保护区, 盐碱地, PGPR, 玉米, 黄杆菌属

Abstract:

【Objective】Soil salinization poses a significant constraint on global agricultural development. However, microorganisms have shown the potential to mitigate salt stress in plants, and long-term intensive cultivation often leads to the unbalance of the beneficial microbial communities in agricultural soils. Isolating plant growth-promoting bacteria from the rhizosphere of wild plants in less disturbed habitats and introducing them into saline-alkali farmland offers a promising strategy to enhance crop tolerance to salt-alkali stress. 【Method】Isolation and culture of rhizospheric bacteria were performed using samples from five wetland plant species collected in the Lalu Wetland National Nature Reserve, Tibet. The isolates were preliminarily identified based on 16S rRNA gene sequencing. Functional characterization was further carried out using specific identification media. Finally, pot experiments were conducted to assess the effects of the selected promising microbial strains on the growth of maize seedlings. 【Result】A total of 260 strains were isolated from the rhizosphere soil of Erigeron canadensis, Galinsoga parviflora, Datura stramonium, Taraxacum mongolicum and Astragalus membranaceus, including 14 species and 21 potential novel species, and the dominant genus was Acinetobacter. 34 strains exhibiting inorganic phosphate-solubilizing capabilities and 47 strains demonstrating siderophore- producing functions were isolated through screening. Among them, strains 3-210 and 3-218 were identified as potential novel species of the genus Flavobacterium through genome analysis, and one key gene for plant hormone signal transduction GS2 was predicted. FeGenie predicted 50 and 71 iron related genes, respectively. No virulence factor genes related to the pathogenic process were detected. At the same time, the results of the pot experiment showed that inoculation of these two strains could improve the agronomic traits, such as plant height and stem diameter of maize seedlings. 3-210 treatment significantly increased the catalase activity and free proline content in leaves, and 3-218 group had significantly higher activities of the catalase and peroxide. 【Conclusion】The rhizospheric soils of plants in the Lalu Wetland National Nature Reserve, Tibet, harbored abundant functional bacterial resources, including multiple strains capable of siderophore production and phosphate solubilization. Notably, two potential novel species of the genus Flavobacterium demonstrated the ability to enhance saline-alkali stress tolerance in maize seedlings.

Key words: Lalu Wetland National Nature Reserve, saline-alkali land, plant growth promoting rhizobacteria, maize, Flavobacterium

杨芾, 韦善君, 张晓霞, 张琳敏, 刘洪秀, 李怡君, 仝亚萍. 西藏拉鲁湿地野生植物根际微生物资源挖掘及其在缓解玉米盐碱胁迫中的作用[J]. 中国农业科学, 2025, 58(20): 4144-4157.

YANG Fu, WEI ShanJun, ZHANG XiaoXia, ZHANG LinMin, LIU HongXiu, LI YiJun, TONG YaPing. Characterization and Utilization of Rhizosphere Microbiota from Wild Plants in Lalu Wetland in Xizang for Alleviating Saline-Alkali Stress in Maize[J]. Scientia Agricultura Sinica, 2025, 58(20): 4144-4157.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2025.20.009

https://www.chinaagrisci.com/CN/Y2025/V58/I20/4144

图/表 7

表1

图1

表2

图2

表3

图3

图4

参考文献 67

[1]	赵耕毛, 杨梦圆, 陈硕, 苏纪康, 吕慧琳, 贾慧昕, 刘兆普. 我国盐碱地治理: 现状、问题与展望. 南京农业大学学报, 2025, 48(1): 14-26.
	ZHAO G M, YANG M Y, CHEN S, SU J K, LÜ H L, JIA H X, LIU Z P. Saline-alkali land management in China: Current situation, problems and prospects. Journal of Nanjing Agricultural University, 2025, 48(1): 14-26. (in Chinese)
[2]	BERG G, ZACHOW C, MÜLLER H, PHILIPPS J, TILCHER R. Next-generation bio-products sowing the seeds of success for sustainable agriculture. Agronomy, 2013, 3(4): 648-656.
[3]	MENDES R, GARBEVA P, RAAIJMAKERS J M. The rhizosphere microbiome: Significance of plant beneficial, plant pathogenic, and human pathogenic microorganisms. FEMS Microbiology Reviews, 2013, 37(5): 634-663. doi: 10.1111/1574-6976.12028 pmid: 23790204
[4]	GOUDA S, KERRY R G, DAS G, PARAMITHIOTIS S, SHIN H S, PATRA J K. Revitalization of plant growth promoting rhizobacteria for sustainable development in agriculture. Microbiological Research, 2018, 206: 131-140. doi: S0944-5013(17)30341-5 pmid: 29146250
[5]	宋立群, 敖静, 刘晓辉, 胡琴琴, 李杨. 根际促生菌在植物根部的定殖研究进展. 应用与环境生物学报, 2025: 1-15. https://link.cnki.net/doi/10.19675/j.cnki.1006-687x.2024.09010.
	SONG L Q, AO J, LIU X H, HU Q Q, LI Y. Research progress on the colonization of plant growth promoting rhizobacteria in plant roots. Chinese Journal of Applied and Environmental Biology, 2025: 1-15. https://link.cnki.net/doi/10.19675/j.cnki.1006-687x.2024.09010. (in Chinese)
[6]	SUN X L, XU Z H, XIE J Y, HESSELBERG-THOMSEN V, TAN T M, ZHENG D Y, STRUBE M L, DRAGOŠ A, SHEN Q R, ZHANG R F, KOVÁCS Á T. Bacillus velezensis stimulates resident rhizosphere Pseudomonas stutzeri for plant health through metabolic interactions. The ISME Journal, 2022, 16(3): 774-787.
[7]	YADAV U, ANAND V, KUMAR S, VERMA I, ANSHU A, PANDEY I A, KUMAR M, BEHERA S K, SRIVASTAVA S, SINGH P C. Bacillus subtilis NBRI-W9 simultaneously activates SAR and ISR against Fusarium chlamydosporum NBRI-FOL7 to increase wilt resistance in tomato. Journal of Applied Microbiology, 2024, 135(3): lxae013.
[8]	SINGH A, PATANI A, PATEL M, VYAS S, VERMA R K, AMARI A, OSMAN H, RATHOD L, ELBOUGHDIRI N, YADAV V K, et al. Tomato seed bio-priming with Pseudomonas aeruginosa strain PAR: A study on plant growth parameters under sodium fluoride stress. Frontiers in Microbiology, 2023, 14: 1330071.
[9]	CONDORI T, ALARCÓN S, HUASASQUICHE L, GARCÍA- BLÁSQUEZ C, PADILLA-CASTRO C, VELÁSQUEZ J, SOLÓRZANO R. Inoculation with Azospirillum brasilense as a strategy to reduce nitrogen fertilization in cultivating purple maize (Zea mays L.) in the inter-Andean valleys of Peru. Microorganisms, 2024, 12(10): 2107.
[10]	QUILBÉ J, LAMY L, BROTTIER L, LELEUX P, FARDOUX J, RIVALLAN R, BENICHOU T, GUYONNET R, BECANA M, VILLAR I, et al. Genetics of nodulation in Aeschynomene evenia uncovers mechanisms of the Rhizobium-legume symbiosis. Nature communications, 2021, 12: 829.
[11]	ZHENG W, WANG N N, QIAN G L, QIAN X, LIU W, HUANG L L. Cross-niche protection of kiwi plant against above-ground canker disease by beneficial rhizosphere Flavobacterium. Communications Biology, 2024, 7: 1458.
[12]	丁方丽, 李嫚, 刘东平, 张忠良, 孙治强, 朴凤植, 申顺善. 植物根际促生菌普城沙雷菌A21-4 对黄瓜生长及土壤微生态的影响. 中国蔬菜, 2018(5): 36-41.
	DING F L, LI M, LIU D P, ZHANG Z L, SUN Z Q, PIAO F Z, SHEN S S. Effects of plant growth promoting rhizobacteria Serratia plymuthica A21-4 on cucumber growth and soil rhizosphere microecology. China Vegetables, 2018(5): 36-41. (in Chinese)
[13]	MUIGANO M N, MAUTI G O, ANAMI S E, ONGUSO J M. Advances and challenges in polyhydroxyalkanoates (PHA) production using Halomonas species: A review. International Journal of Biological Macromolecules, 2025, 309(Pt 1): 142850.
[14]	YANG X X, YUAN R W, YANG S Y, DAI Z A, DI N, YANG H J, HE Z L, WEI M. A salt-tolerant growth-promoting phyllosphere microbial combination from mangrove plants and its mechanism for promoting salt tolerance in rice. Microbiome, 2024, 12(1): 270. doi: 10.1186/s40168-024-01969-9 pmid: 39707568
[15]	周宇鹏, 蔡奕, 周念清, 杜喜玲, 李娜, 邢婧文. 河流潜流带控氮微生物特性及影响因素研究进展. 地球科学前沿(汉斯), 2025(3): 281-292.
	ZHOU Y P, CAI Y, ZHOU N Q, DU X L, LI N, XING J W. Progress in characteristics and influencing factors of microorganisms controlling nitrogen cycle in the hyporheic zone of a river. Advances in Geosciences, 2025(3): 281-292. (in Chinese)
[16]	宋雅荣, 常单娜, 周国朋, 高嵩涓, 段廷玉, 曹卫东. 解磷细菌活化水稻土中低品位磷矿粉的效果与机制. 中国农业科学, 2024, 57(6): 1102-1116. doi:10.3864/j.issn.0578-1752.2024.06.007.
	SONG Y R, CHANG D N, ZHOU G P, GAO S J, DUAN T Y, CAO W D. Effect and mechanism of phosphate-solubilizing bacterial on activating of low-grade phosphate rock powder in red paddy soil. Scientia Agricultura Sinica, 2024, 57(6): 1102-1116. doi:10.3864/ j.issn.0578-1752.2024.06.007. (in Chinese)
[17]	张淑静, 张淑凤, 杨萍, 肖琳, 曲田丽, 张保华, 牛赡光, 杜丰玉. 山东湿地生防菌筛选及解淀粉芽孢杆菌JQ-3的防病促生作用. 中国植保导刊, 2024, 44(5): 16-21.
	ZHANG S J, ZHANG S F, YANG P, XIAO L, QU T L, ZHANG B H, NIU S G, DU F Y. Screening of biocontrol bacteria from wetland of Shandong and the disease control and growth promotion effect of Bacillus amyloliquefaciens JQ-3. China Plant Protection, 2024, 44(5): 16-21. (in Chinese)
[18]	曹莹. 湿地根际微生物对磷的转化释放及植物吸收的影响[D]. 南京: 东南大学, 2018.
	CAO Y. Effects of wetland rhizosphere microorganisms on phosphorus transformation and release and plant uptake[D]. Nanjing: Southeast University, 2018. (in Chinese)
[19]	金小茜. 天津永定河故道国家湿地公园景观植物内生细菌功能发掘研究[D]. 北京: 中国林业科学研究院, 2022.
	JIN X Q. Functional exploration of endophytic bacteria in landscape plants of Yongding river national wetland park in Tianjin[D]. Beijing: Chinese Academy of Forestry, 2022. (in Chinese)
[20]	李青青, 张芮, 高彦婷, 张红娟, 刘柯含. 耐盐碱解磷菌的溶磷效果及其对黄豆萌发的影响. 微生物学通报, 2024, 51(11): 4574-4589.
	LI Q Q, ZHANG R, GAO Y T, ZHANG H J, LIU K H. Phosphorus solubilization effect of saline-tolerant phosphorus-solubilizing bacteria and its effect on soybean germination. Microbiology China, 2024, 51(11): 4574-4589. (in Chinese)
[21]	CUI K P, XU T, CHEN J W, YANG H Y, LIU X M, ZHUO R, PENG Y H, TANG W, WANG R, CHEN L S, et al. Siderophores, a potential phosphate solubilizer from the endophyte Streptomyces sp. CoT10, improved phosphorus mobilization for host plant growth and rhizosphere modulation. Journal of Cleaner Production, 2022, 367: 133110.
[22]	何建清, 张格杰, 岳海梅. 拉鲁湿地自然保护区放线菌组成分析及生物活性测定. 微生物学杂志, 2009, 29(4): 6-10.
	HE J Q, ZHANG G J, YUE H M. Diversity and bioactivity analysis of actinomycetes isolated from lhalu wetland nature reserve. Journal of Microbiology, 2009, 29(4): 6-10. (in Chinese)
[23]	德吉, 苏延山, 龙琦炜. 西藏拉鲁湿地水体产胞外酶酵母菌筛选及其与理化因子相关性分析. 高原科学研究, 2018, 2(1): 20-30, 37.
	DE J, SU Y S, LONG Q W. Screening of extracellular enzymes producing yeasts and analysis on its relationship to water environmental factors in Lhalu Wetland. Plateau Science Research, 2018, 2(1): 20-30, 37. (in Chinese)
[24]	郭小芳, 德吉, 龙琦炜, 白斌锦, 王豪杰, 曹亚璞. 西藏拉鲁湿地水体酵母菌多样性及其与理化因子相关性. 微生物学报, 2018, 58(7): 1167-1181.
	GUO X F, DE J, LONG Q W, BAI B J, WANG H J, CAO Y P. Spatial dynamics of yeast community and its relationship with environmental factors in Lhalu Wetland, Tibet. Acta Microbiologica Sinica, 2018, 58(7): 1167-1181. (in Chinese)
[25]	荣良燕, 姚拓, 赵桂琴, 柴强, 席琳乔, 王小利. 产铁载体PGPR菌筛选及其对病原菌的拮抗作用. 植物保护, 2011, 37(1): 59-64.
	RONG L Y, YAO T, ZHAO G Q, CHAI Q, XI L Q, WANG X L. Screening of siderophore-producing PGPR bacteria and their antagonism against the pathogens. Plant Protection, 2011, 37(1): 59-64. (in Chinese)
[26]	韩嘉诚, 朱宏图, 杨芾, 郭捷, 马晓彤, 张晓霞. 中国农业微生物菌种保藏管理中心大豆根瘤菌精准评价. 微生物学报, 2025, 65(4): 1667-1683.
	HAN J C, ZHU H T, YANG F, GUO J, MA X T, ZHANG X X. Accurate evaluation of soybean rhizobia preserved in the Agricultural Culture Collection of China. Acta Microbiologica Sinica, 2025, 65(4): 1667-1683. (in Chinese)
[27]	杨芾, 王辉, 王琴, 姜春前, 周妍旭, 李潞滨. 浙江地区毛竹林根际土壤促生细菌筛选及促生效应研究. 南京林业大学学报(自然科学版), 2025, 49(2): 83-90. doi: 10.12302/j.issn.1000-2006.202307015
	YANG F, WANG H, WANG Q, JIANG C Q, ZHOU Y X, LI L B. Screening of growth-promoting bacteria in the rhizosphere soil of Phyllostachys edulis and their growth-promoting effects in Zhejiang Province. Journal of Nanjing Forestry University (Natural Sciences Edition), 2025, 49(2): 83-90. (in Chinese)
[28]	WOESE C R, FOX G E. Phylogenetic structure of the prokaryotic domain: The primary Kingdoms. PNAS, 1977, 74(11): 5088-5090. pmid: 270744
[29]	CHALITA M, KIM Y O, PARK S, OH H S, CHO J H, MOON J, BAEK N, MOON C, LEE K, YANG J, et al. EzBioCloud: A genome-driven database and platform for microbiome identification and discovery. International Journal of Systematic and Evolutionary Microbiology, 2024, 74(6): 006421.
[30]	KUMAR S, STECHER G, LI M, KNYAZ C, TAMURA K. MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 2018, 35(6): 1547-1549. doi: 10.1093/molbev/msy096 pmid: 29722887
[31]	EDGAR R C. Muscle5: High-accuracy alignment ensembles enable unbiased assessments of sequence homology and phylogeny. Nature Communications, 2022, 13: 6968. doi: 10.1038/s41467-022-34630-w pmid: 36379955
[32]	KIMURA M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 1980, 16(2): 111-120. doi: 10.1007/BF01731581 pmid: 7463489
[33]	东秀珠, 蔡妙英. 常见细菌系统鉴定手册. 北京: 科学出版社, 2001.
	DONG X Z, CAI M Y. Handbook of Identification of Common Bacterial Systems. Beijing: Science Press, 2001. (in Chinese)
[34]	LIU H L, XIN B Y, ZHENG J S, ZHONG H, YU Y, PENG D H, SUN M. Build a bioinformatics analysis platform and apply it to routine analysis of microbial genomics and comparative genomics. Protocol Exchange, 2021.
[35]	BANKEVICH A, NURK S, ANTIPOV D, GUREVICH A A, DVORKIN M, KULIKOV A S, LESIN V M, NIKOLENKO S I, PHAM S, PRJIBELSKI A D, et al. SPAdes: A new genome assembly algorithm and its applications to single-cell sequencing. Journal of Computational Biology, 2012, 19(5): 455-477. doi: 10.1089/cmb.2012.0021 pmid: 22506599
[36]	PARKS D H, IMELFORT M, SKENNERTON C T, HUGENHOLTZ P, TYSON G W. CheckM: Assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes. Genome Research, 2015, 25(7): 1043-1055. doi: 10.1101/gr.186072.114 pmid: 25977477
[37]	PRITCHARD L, GLOVER R H, HUMPHRIS S, ELPHINSTONE J G, TOTH I K. Genomics and taxonomy in diagnostics for food security: Soft-rotting enterobacterial plant pathogens. Analytical Methods, 2016, 8(1): 12-24.
[38]	MEIER-KOLTHOFF J P, CARBASSE J S, PEINADO-OLARTE R L, GÖKER M. TYGS and LPSN: A database tandem for fast and reliable genome-based classification and nomenclature of prokaryotes. Nucleic Acids Research, 2022, 50(D1): D801-D807.
[39]	KANEHISA M, FURUMICHI M, SATO Y, MATSUURA Y, ISHIGURO-WATANABE M. KEGG: Biological systems database as a model of the real world. Nucleic Acids Research, 2025, 53(D1): D672-D677.
[40]	HUERTA-CEPAS J, SZKLARCZYK D, HELLER D, HERNÁNDEZ- PLAZA A, FORSLUND S K, COOK H, MENDE D R, LETUNIC I, RATTEI T, JENSEN L J, VON MERING C, BORK P. eggNOG 5.0: A hierarchical, functionally and phylogenetically annotated orthology resource based on 5090 organisms and 2502 viruses. Nucleic Acids Research, 2019, 47(D1): D309-D314.
[41]	GARBER A I, NEALSON K H, OKAMOTO A, MCALLISTER S M, CHAN C S, BARCO R A, MERINO N. FeGenie: A comprehensive tool for the identification of iron genes and iron gene neighborhoods in genome and metagenome assemblies. Frontiers in Microbiology, 2020, 11: 37. doi: 10.3389/fmicb.2020.00037 pmid: 32082281
[42]	张静, 高文博, 晏林, 张宗文, 周海涛, 吴斌. 燕麦种质资源耐盐碱性鉴定评价及耐盐碱种质筛选. 作物学报, 2023, 49(6): 1551-1561. doi: 10.3724/SP.J.1006.2023.21032
	ZHANG J, GAO W B, YAN L, ZHANG Z W, ZHOU H T, WU B. Identification and evaluation of salt-alkali tolerance and screening of salt-alkali tolerant germplasm of oat (Avena sativa L.). Acta Agronomica Sinica, 2023, 49(6): 1551-1561. (in Chinese) doi: 10.3724/SP.J.1006.2023.21032
[43]	刘谢香, 常汝镇, 关荣霞, 邱丽娟. 大豆出苗期耐盐性鉴定方法建立及耐盐种质筛选. 作物学报, 2020, 46(1): 1-8.
	LIU X X, CHANG R Z, GUAN R X, QIU L J. Establishment of screening method for salt tolerant soybean at emergence stage and screening of tolerant germplasm. Acta Agronomica Sinica, 2020, 46(1): 1-8. (in Chinese)
[44]	KIM M, OH H S, PARK S C, CHUN J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. International Journal of Systematic and Evolutionary Microbiology, 2014, 64(Pt 2): 346-351. doi: 10.1099/ijs.0.059774-0 pmid: 24505072
[45]	刘晓丽, 姚宏武, 李密, 李珊, 刘运喜. 泛耐药鲍曼不动杆菌研究进展. 中华医院感染学杂志, 2025, 35(13): 2050-2056.
	LIU X L, YAO H W, LI M, LI S, LIU Y X. Progress of research on extensively drug-resistant Acinetobacter baumannii. Chinese Journal of Nosocomiology, 2025, 35(13): 2050-2056. (in Chinese)
[46]	张新飞, 佘木子, 李晗玉, 敬松, 姜惠娜, 高浩, 朱延晓, 付娟娟. 琥珀酸黄杆菌促生机理及其对多年生黑麦草生长和抗逆性的生理调控作用. 草地学报, 2021, 29(8): 1704-1711. doi: 10.11733/j.issn.1007-0435.2021.08.013
	ZHANG X F, SHE M Z, LI H Y, JING S, JIANG H N, GAO H, ZHU Y X, FU J J. Growth promotion mechanisms of Flavobacterium succinicans and their physiological regulation on the growth and stress tolerance in Lolium perenne. Acta Agrestia Sinica, 2021, 29(8): 1704-1711. (in Chinese)
[47]	贾南豫, 朱水英, 蒋栋, 杨盛蝶, 牛国庆, 韩鑫, 文涛, 袁军, 沈其荣. 施用液体有机肥对干旱胁迫下玉米和小麦生长及根际微生物的影响. 南京农业大学学报, 2025, 48(5): 1060-1069.
	JIA N Y, ZHU S Y, JIANG D, YANG S D, NIU G Q, HAN X, WEN T, YUAN J, SHEN Q R. The effect of liquid organic fertilizer on the growth and rhizosphere microorganisms of corn and wheat under drought stress. Journal of Nanjing Agricultural University, 2025, 48(5): 1060-1069. (in Chinese)
[48]	李新鹏. 小麦GS2基因的多样性及其与农艺性状关联分析的研究[D]. 北京: 中国科学院遗传与发育生物学研究所, 2008.
	LI X P. Study on the diversity of wheat GS2 gene and its correlation analysis with agronomic traits[D]. Beijing: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 2008. (in Chinese)
[49]	刘丽娟, 王红莉, 王亚军, 任燕, 王庆, 石存斌, 张德锋. 笋壳鱼源柱状黄杆菌的分离鉴定及致病性和药物敏感性. 南方农业学报, 2023, 54(2): 638-646.
	LIU L J, WANG H L, WANG Y J, REN Y, WANG Q, SHI C B, ZHANG D F. Isolation, identification, pathogenicity and drug susceptibility of Flavobacterium columnare from Oxyeleotris marmoratus. Journal of Southern Agriculture, 2023, 54(2): 638-646. (in Chinese)
[50]	AN J X, LIU C, WANG Q, YAO M J, RUI J P, ZHANG S H, LI X Z. Soil bacterial community structure in Chinese wetlands. Geoderma, 2019, 337: 290-299. doi: 10.1016/j.geoderma.2018.09.035
[51]	SONG S S, ZHANG C, GAO Y, ZHU X Y, WANG R H, WANG M D, ZHENG Y L, HOU L J, LIU M, WU D M. Responses of wetland soil bacterial community and edaphic factors to two-year experimental warming and Spartina alterniflora invasion in Chongming Island. Journal of Cleaner Production, 2020, 250: 119502.
[52]	马骁. 海洋及洞穴环境细菌新种多相分类及一株药用植物内生链霉菌新种次级代谢产物研究[D]. 遵义: 遵义医科大学, 2024.
	MA X. Polyphase classification of new bacterial species in marine and cave environments and study on secondary metabolites of a new species of endophytic Streptomyces in medicinal plants[D]. Zunyi: Zunyi Medcial University, 2024. (in Chinese)
[53]	马雪晴, 冀傲冉, 郑娇莉, 曹春霞, 龚艳, 黄大野, 王蓓蓓. 植物根际促生菌促生机制及其应用研究进展. 中国农业科技导报(中英文), 2025, 27(2): 13-23.
	MA X Q, JI A R, ZHENG J L, CAO C X, GONG Y, HUANG D Y, WANG B B. Research progress on growth-promoting mechanism and application of plant growth-promoting rhizobacteria. Journal of Agricultural Science and Technology, 2025, 27(2): 13-23. (in Chinese)
[54]	张丽珍, 樊晶晶, 牛伟, 李涛, 吴荣海, 金益杰, 鹿茸. 盐碱地柠条根围土中黑曲霉的分离鉴定及解磷能力测定. 生态学报, 2011, 31(24): 7571-7578.
	ZHANG L Z, FAN J J, NIU W, LI T, WU R H, JIN Y J, LU R. Isolation of phosphate solubilizing fungus(Aspergillus niger) from Caragana rhizosphere and its potential for phosphate solubilization. Acta Ecologica Sinica, 2011, 31(24): 7571-7578. (in Chinese)
[55]	姜焕焕, 祁佩时, 王通, 迟晓元, 陈明娜. 花生根际多功能固氮菌的分离及其耐盐碱特性研究. 生物技术通报, 2019, 35(3): 24-30. doi: 10.13560/j.cnki.biotech.bull.1985.2018-0770
	JIANG H H, QI P S, WANG T, CHI X Y, CHEN M N. Screening of multi-function nitrogen-fixing bacteria in peanut rhizosphere and their tolerances to saline. Biotechnology Bulletin, 2019, 35(3): 24-30. (in Chinese)
[56]	SHIRMOHAMMADI E, ALI ALIKHANI H, ALI POURBABAEI A, ETESAMI H. Improved phosphorus (P) uptake and yield of rainfed wheat fed with P fertilizer by drought-tolerant phosphate-solubilizing fluorescent pseudomonads strains: A field study in drylands. Journal of Soil Science and Plant Nutrition, 2020, 20(4): 2195-2211.
[57]	WU F, LI J R, CHEN Y L, ZHANG L P, ZHANG Y, WANG S, SHI X, LI L, LIANG J S. Effects of phosphate solubilizing bacteria on the growth, photosynthesis, and nutrient uptake of Camellia oleifera Abel. Forests, 2019, 10(4): 348.
[58]	SAFIRZADEH S, CHOROM M, ENAYATIZAMIR N. Effect of phosphate solubilising bacteria (Enterobacter cloacae) on phosphorus uptake efficiency in sugarcane (Saccharum officinarum L.). Soil Research, 2019, 57(4): 333-341.
[59]	ZHANG T T, HU F, MA L. Phosphate-solubilizing bacteria from safflower rhizosphere and their effect on seedling growth. Open Life Sciences, 2019, 14: 246-254.
[60]	刘金涛, 姚凡, 李臻园, 李庆懋, 黄立钰. 植物铁素吸收机制研究进展. 热带农业科学, 2022, 42(5): 26-33.
	LIU J T, YAO F, LI Z Y, LI Q M, HUANG L Y. Advances on the mechanism of iron absorption in plants. Chinese Journal of Tropical Agriculture, 2022, 42(5): 26-33. (in Chinese)
[61]	CAVITE H J M, MACTAL A G, EVANGELISTA E V, CRUZ J A. Growth and yield response of upland rice to application of plant growth-promoting rhizobacteria. Journal of Plant Growth Regulation, 2021, 40(2): 494-508.
[62]	李艳梅, 王琼瑶, 涂卫国, 崔永亮, 钟玘狄, 李俐珩, 陈强, 余秀梅. 镍胁迫下产铁载体细菌对花生的促生性. 微生物学通报, 2017, 44(8): 1882-1890.
	LI Y M, WANG Q Y, TU W G, CUI Y L, ZHONG Q D, LI L H, CHEN Q, YU X M. Growth promoting activity of siderophore secreting bacteria for peanut plant under nickel stress. Microbiology China, 2017, 44(8): 1882-1890. (in Chinese)
[63]	SUN Y X, WU J L, SHANG X Y, XUE L G, JI G Y, CHANG S J, NIU J B, EMANEGHEMI B. Screening of siderophore-producing bacteria and their effects on promoting the growth of plants. Current Microbiology, 2022, 79(5): 150. doi: 10.1007/s00284-022-02777-w pmid: 35396958
[64]	杨晓蕾, 李建宏, 姚拓, 梅丽娜, 李琦, 白洁, 朱青青, 赵晓倩, 赵树栋. 5株植物根际促生菌功能特性及培养条件. 草业科学, 2022, 39(1): 30-38.
	YANG X L, LI J H, YAO T, MEI L N, LI Q, BAI J, ZHU Q Q, ZHAO X Q, ZHAO S D. Functional characteristics and culture conditions of five plant growth-promoting rhizobacteria strains. Pratacultural Science, 2022, 39(1): 30-38. (in Chinese)
[65]	SADEGHI A, KARIMI E, DAHAJI P A, JAVID M G, DALVAND Y, ASKARI H. Plant growth promoting activity of an auxin and siderophore producing isolate of Streptomyces under saline soil conditions. World Journal of Microbiology and Biotechnology, 2012, 28(4):1503-1509.
[66]	吴昕劢, 栗晗, 王经邦, 贾保磊, 姚燕来, 郑华宝, 刘妍. 耐盐菌株筛选及其对盐胁迫下番茄种子萌发促进效应解析. 南京农业大学学报, 2025: 1-14. https://link.cnki.net/urlid/32.1148.S.20250517.1811.002.
	WU X M, LI H, WANG J B, JIA B L, YAO Y L, ZHENG H B, LIU Y. Screening of salt-tolerant bacterial strains and analysis of their effects on promoting tomato seed germination under salt stress. Journal of Nanjing Agricultural University, 2025: 1-14. https://link.cnki.net/urlid/32.1148.S.20250517.1811.002. (in Chinese)
[67]	王博瑞, 罗汶婧, 马红彬, 李慧萍. 根际促生细菌调控植物响应盐胁迫的作用机制. 微生物学通报, 2025: 1-20. https://doi.org/10.13344/j.microbiol.china.250187.
	WANG B R, LUO W J, MA H B, LI H P. Mechanism of regulation of plant response to salt stress by plant growth-promoting rhizobacteria. Microbiology China, 2025: 1-20. https://doi.org/10.13344/j.microbiol.china.250187. (in Chinese)

处理组别 Group	种子萌发试验 Seed germination	幼苗生长试验 Seedling growth
CK+	R2A液体培养基浸种+无盐碱滤纸 R2A broth seed soaking and salt-alkali-free filter paper	R2A液体培养基浸种+60 mL无菌水 R2A broth seed soaking and 60 mL sterilized water
CK-	R2A液体培养基浸种+盐碱浸润滤纸 R2A broth seed soaking and salt-alkal filter paper	R2A液体培养基浸种+60 mL盐碱溶液 R2A broth seed soaking and 60 mL salt-alkali solution
3-210	菌液3-210浸种+盐碱浸润滤纸 Suspension 3-210 seed soaking and salt-alkali filter paper	菌液3-210浸种+60 mL盐碱溶液 Suspension 3-210 seed soaking and 60 mL salt-alkali solution
3-218	菌液3-218浸种+盐碱浸润滤纸 Suspension 3-218 seed soaking and salt-alkali filter paper	菌液3-218浸种+60 mL盐碱溶液 Suspension 3-218 seed soaking and 60 mL salt-alkali solution

处理组别 Group	发芽势 Germination potential (%)	发芽率 Germination rate (%)
CK+	64.28±20.24a	78.57±12.60a
CK-	51.78±24.20b	57.41±18.84c
3-210	59.26±18.84a	64.28±20.25b
3-218	55.54±20.41a	61.90±18.54b

处理组别 Group	盐害程度 Salt damage degree (%)					伤害指数 Injury index (%)
处理组别 Group	1级 Grade 1	2级 Grade 2	3级 Grade 3	4级 Grade 4	5级 Grade 5	伤害指数 Injury index (%)
CK-	50.00	0	27.78	11.11	11.11	46.67
3-210	55.56	11.11	16.67	11.11	5.56	40.00
3-218	55.56	5.56	16.67	11.11	11.11	29.12

西藏拉鲁湿地野生植物根际微生物资源挖掘及其在缓解玉米盐碱胁迫中的作用

Characterization and Utilization of Rhizosphere Microbiota from Wild Plants in Lalu Wetland in Xizang for Alleviating Saline-Alkali Stress in Maize

RichHTML

PDF

赞

可视化

摘要/Abstract

引用本文

使用本文

图/表 7

参考文献 67

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	韦文华, 李盼, 邵冠贵, 樊志龙, 胡发龙, 范虹, 何蔚, 柴强, 殷文, 赵连豪. 西北灌区青贮玉米产量及品质对减量灌水与有机无机肥配施的响应[J]. 中国农业科学, 2025, 58(8): 1521-1534.
[2]	薛钰琦, 赵继玉, 孙旺胜, 任佰朝, 赵斌, 刘鹏, 张吉旺. 不同氮素形态对夏玉米产量和品质的影响[J]. 中国农业科学, 2025, 58(8): 1535-1549.
[3]	陈桂平, 李盼, 邵冠贵, 吴霞玉, 殷文, 赵连豪, 樊志龙, 胡发龙. 减量灌水与有机无机肥配施对青贮玉米吐丝期后叶片持绿特性的调控作用[J]. 中国农业科学, 2025, 58(7): 1381-1396.
[4]	岳润清, 李文兰, 丁照华, 孟昭东. 转基因复合抗虫耐除草剂玉米LD05的分子特征及抗性评价[J]. 中国农业科学, 2025, 58(7): 1269-1283.
[5]	赵耀, 程前, 徐田军, 刘正, 王荣焕, 赵久然, 陆大雷, 李从锋. 高密度条件下株型改良对春玉米根-冠特征及籽粒产量的影响[J]. 中国农业科学, 2025, 58(7): 1296-1310.
[6]	邹晓威, 夏蕾, 朱晓敏, 孙辉, 周琦, 齐霁, 张亚封, 郑岩, 姜兆远. 基于转录组测序的玉米瘤黑粉菌UM01240过表达菌株诱导玉米抗病性分析[J]. 中国农业科学, 2025, 58(6): 1116-1130.
[7]	周广飞, 马亮, 马璐, 张舒钰, 章慧敏, 宋旭东, 张振良, 陆虎华, 郝德荣, 冒宇翔, 薛林, 陈国清. 玉米苞叶性状全基因组关联分析[J]. 中国农业科学, 2025, 58(3): 431-442.
[8]	王佳芯, 胡静一, 张巍, 魏骞, 王涛, 王小林, 张雄, 张盼盼. 覆膜方式对间作玉米光合物质生产及水分利用效率的影响[J]. 中国农业科学, 2025, 58(3): 460-477.
[9]	张方方, 宋启龙, 高娜, 白炬, 李阳, 岳善超, 李世清. 长期覆盖对黄土高原春玉米产量、土壤碳氮组分和碳氮库相关指数的影响[J]. 中国农业科学, 2025, 58(3): 507-519.
[10]	吕国华, 王庆锁, 宋佳珅, 李玉义, 梅旭荣. 我国盐碱地综合利用的生态保护战略[J]. 中国农业科学, 2025, 58(20): 4047-4053.
[11]	武书羽, 衡燕芳, 于太飞, 王世佳, 于思佳, 李园, 胡正, 张辉, 孙现军, 黎亮, 姜奇彦. 玉米自然群体苗期耐盐性鉴定及耐盐相关基因分析[J]. 中国农业科学, 2025, 58(20): 4085-4099.
[12]	曲海悦, 韩洁楠, 李冉, 张泽, 刘倩倩, 郝转芳, 翁建峰, 张德贵, 周志强, 许振南, 荣子国, 王菊英, 雍洪军, 李明顺. 新育中单系列玉米杂交种与亲本自交系的耐盐性评价[J]. 中国农业科学, 2025, 58(20): 4100-4116.
[13]	韩哲群, 苏莹, 高奇奇, 刘美英, 贾昂元, 张海睿, 南珊珊, 徐勤政, 王强, 王利军, 武雪萍. 智能滴灌水肥耦合模式实现土壤节水控盐与向日葵产量品质协同提升[J]. 中国农业科学, 2025, 58(20): 4158-4177.
[14]	张梦璇, 常芳弟, 魏禾雅, 李晓红, 伍林美, 张宏媛. 盐碱地麦后复种绿肥与氮肥减施对小麦产量、环境足迹及经济效益的协同效应[J]. 中国农业科学, 2025, 58(20): 4216-4230.
[15]	李明珠, 田荣荣, 李然, 王淑娟, 王伟妮, 王婧, 刘俊梅, 刘嘉, 李玉义, 徐立珍, 李彦, 赵永敢. 钙/铝基改良剂对河套灌区盐碱土质量及向日葵产量的影响[J]. 中国农业科学, 2025, 58(20): 4231-4245.