秧苗富锌对不同水稻品种镉积累及相关转运基因的影响

doi:10.3864/j.issn.0578-1752.2022.17.001

摘要/Abstract

摘要：

【目的】秧苗富锌是发挥水稻锌-镉拮抗效应的一种新模式,研究不同水稻品种对秧苗富锌降镉效应的响应差异及潜在机制,为该模式的推广应用奠定基础。【方法】选择武运粳21号、浙粳优1578、黄华占、徽两优丝苗等4个品种开展水培富锌和盆栽验证试验,研究不同品种锌富集的动态特征及移栽后水稻组织对镉的吸收转运差异,通过分析根系相关镉转运基因的表达状况探究秧苗富锌降镉效应的种间差异机制。【结果】锌培养对水稻秧苗的生长发育无明显影响,但不同品种幼苗锌富集能力差异明显,浙粳优1 578幼苗锌积累量（230 μg/株）最高,武运粳21号次之（124 μg/株）,黄华占和徽两优丝苗相对较低（85.1—95.0 μg/株）。浙粳优1578幼苗根系镉转运基因对富锌较为敏感,OsZIP7表达下调48.7%,OsZIP1表达上调81.3%;武运粳21号幼苗根系OsIRT1和OsZIP7表达分别下调35.9%和35.0%,OsZIP1表达上调31.1%;而黄华占和徽两优丝苗幼苗根系镉转运基因对富锌较不敏感。秧苗富锌使分蘖期武运粳21号根系和茎叶镉浓度分别下降26.7%和36.7%;浙粳优1578根系和茎叶镉浓度分别下降32.0%和40.0%,根系-茎叶镉转运系数显著下降12.0%。秧苗富锌使武运粳21号、浙粳优1578和黄华占糙米镉含量分别下降37.5%、36.7%和25.3%,而对徽两优丝苗糙米镉含量无明显影响,表明秧苗富锌对水稻降镉效应存在明显的种间差异。相关分析显示,糙米Cd浓度与茎叶Cd浓度呈显著正相关,而茎叶Cd浓度与根系和茎叶Zn浓度呈显著负相关,表明水稻内部Zn、Cd拮抗作用明显。根系和茎叶Zn浓度均与OsZIP7和OsIRT1的相对表达量呈显著负相关,与OsZIP1的相对表达量呈显著正相关关系,表明秧苗富锌主要通过调控根系OsZIP1、OsZIP7和OsIRT1的表达,从而间接影响糙米镉含量。【结论】不同品种幼苗根系镉转运基因对富锌的响应差异明显,浙粳优1578和武运粳21号较为敏感,故而糙米镉含量下降幅度高于其他品种。

关键词: 土壤镉污染, 拮抗效应, 转运基因, 水稻糙米, 相关分析

Abstract:

【Objective】 Rice seedlings enriched with zinc (Zn) is a new method to antagonize cadmium (Cd) within rice, studying the various effects and underlying mechanisms among different rice cultivars is useful to provide a basis for the application of this method. 【Method】 Hydroponic and pot experiments were conducted to test the effects of the Zn enrichment in four cultivars including the Wuyunjing21, Zhejingyou1578, Huanghuazhan, and Huiliangyousimiao. Dynamics of Zn accumulation in different cultivars, and variations of Cd uptake and transport in rice tissues were investigated. Furthermore, the expressions of the Cd-related transporter genes of the root were analyzed to explore the underlying mechanisms responsible for the various effects in different cultivars. 【Result】 Results showed that the Zn cultivation didn’t affect the growth of all rice seedlings. Zn accumulations varied obviously among different cultivars, the Zhejingyou1578 had the highest Zn level (230 μg Zn per plant), followed by the Wuyunjing21 (124 μg Zn per plant), the Huanghuazhan and Huiliangyousimiao had the low levels (85.1-95.0 μg Zn per plant). Zn enrichment greatly influenced the expression of Cd-related transporters in the Zhejingyou1578, the OsZIP7 was down-regulated by 48.7% and OsZIP1 was up-regulated by 81.3%. The expressions of the OsIRT1 and OsZIP7 in the Wuyunjing21 were also down-regulated by 35.9% and 35.0%, respectively, and the OsZIP1 was up-regulated by 31.1%. However, the Cd-related transporters in the Huanghuazhan and Huiliangyousimiao were insensitive to Zn enrichment. As a result, Zn enrichment significantly reduced Cd concentrations in the root and shoot by 26.7% and 36.7% of the Wuyunjing21, and 32.0% and 40.0% of the Zhejingyou1578 with a 12.0% inhibition on the Cd transport, respectively. Furthermore, brown rice Cd were reduced by 37.5%, 36.7% and 25.3% in the Wuyunjing21, Zhejingyou1578, and Huanghuazhan, respectively, while no difference occurred in the Huiliangyousimiao, revealing various effects on reducing Cd among different cultivars induced by the Zn enrichment. Correlation analysis showed that the brown rice Cd was negatively correlated with the shoot Cd which was negatively correlated with the root and shoot Zn, revealing a significant antagonism between Zn and Cd within rice. However, the root and shoot Zn were negatively correlated with the OsZIP7 and OsIRT1 and positively correlated with the OsZIP1 expression, indicating that the Zn enrichment within seedlings influenced the brown rice Cd mainly through regulating the expressions of the OsZIP1, OsZIP7 and OsIRT1. 【Conclusion】 The regulations on Cd-related transporters through Zn enrichment were varied among different cultivars, and the Zhejingyou1578 and Wuyunjing21 were more sensitive, so that the reductions of brown rice Cd were higher than other cultivars.

Key words: soil Cd contamination, antagonistic effect, transporter gene, brown rice, correlation analysis

姜晓婷,黄高翔,熊小英,黄芸培,丁昌峰,丁明军,王鹏. 秧苗富锌对不同水稻品种镉积累及相关转运基因的影响[J]. 中国农业科学, 2022, 55(17): 3267-3277.

JIANG XiaoTing,HUANG GaoXiang,XIONG XiaoYing,HUANG YunPei,DING ChangFeng,DING MingJun,WANG Peng. Effects of Seedlings Enriched with Zinc on Cadmium Accumulations and Related Transporter Genes Expressions in Different Rice Cultivars[J]. Scientia Agricultura Sinica, 2022, 55(17): 3267-3277.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2022.17.001

https://www.chinaagrisci.com/CN/Y2022/V55/I17/3267

图/表 7

表1

图1

图2

图3

图4

图5

图6

参考文献 37

[1]	张妍, 张磊, 程红光, 孙海旭, 崔祥芬. 南方某矿区土壤镉污染及作物健康风险研究. 农业环境科学学报, 2020, 39(12): 2752-2761.
	ZHANG Y, ZHANG L, CHENG H G, SUN H X, CUI X F. Soil cadmium pollution and crop health risk in a mining area in south China. Journal of Agro-Environment Science, 2020, 39(12): 2752-2761. (in Chinese)
[2]	林小兵, 周利军, 王惠明, 刘晖, 武琳, 俞莹, 胡敏, 何波, 周青辉, 黄欠如. 不同水稻品种对重金属的积累特性. 环境科学, 2018, 39(11): 5198-5206.
	LIN X B, ZHOU L J, WANG H M, LIU H, WU L, YU Y, HU M, HE B, ZHOU Q H, HUANG Q R. Accumulation of heavy metals in different rice varieties. Environmental Science, 2018, 39(11): 5198-5206. (in Chinese)
[3]	马娇阳, 保欣晨, 张振宁, 王成尘, 崔道雷, 向萍. 正常肝细胞和肝癌细胞对镉暴露毒性响应差异分析. 环境科学研究, 2022, 35(1): 257-264.
	MA J Y, BAO X C, ZHANG Z N, WANG C C, CUI D L, XIANG P. Cellular responses of normal and cancerous hepatic cells to cadmium exposure. Research of Environmental Sciences, 2022, 35(1): 257-264. (in Chinese)
[4]	SHEN X M, LIU W, CHEN Y J, GUO Y F, GAO M, CHEN W P, LIU Y J, LIU S J. Diagnostic significance of metallothionein members in recognizing cadmium exposure in various organs under low-dose exposure. Chemosphere, 2019, 229: 32-40. doi: 10.1016/j.chemosphere.2019.04.192
[5]	WANG P, CHEN H P, KOPITTKE P M, ZHAO F J. Cadmium contamination in agricultural soils of China and the impact on food safety. Environmental Pollution, 2019, 249: 1038-1048. doi: 10.1016/j.envpol.2019.03.063
[6]	ZHAO F J, MA Y B, ZHU Y G, TANG Z, MCGRATH S P. Soil contamination in China: Current status and mitigation strategies. Environmental Science & Technology, 2015, 49(2): 750-759. doi: 10.1021/es5047099
[7]	LEE S, AN G. Over-expression of OsIRT1 leads to increased iron and zinc accumulations in rice. Plant Cell and Environment, 2009, 32(4): 408-416. doi: 10.1111/j.1365-3040.2009.01935.x
[8]	TAKAHASHI R, ISHIMARU Y, SHIMO H, OGO Y, SENOURA T, NISHIZAWA N K, NAKANISHI H. The OsHMA2 transporter is involved in root to-shoot translocation of Zn and Cd in rice. Plant Cell and Environment, 2012, 35(11): 1948-1957. doi: 10.1111/j.1365-3040.2012.02527.x
[9]	SASAKI A, YAMAJI N, MA J F. Overexpression of OsHMA3 enhances Cd tolerance and expression of Zn transporter genes in rice. Journal of Experimental Botany, 2014, 65(20): 6013-6021. doi: 10.1093/jxb/eru340
[10]	LIU X S, FENG S J, ZHANG B Q, WANG M Q, CAO H W, RONO J K, CHEN X, YANG Z M. OsZIP1 functions as a metal efflux transporter limiting excess zinc, copper and cadmium accumulation in rice. BMC Plant Biology, 2019, 19(1): 1-16. doi: 10.1186/s12870-018-1600-2
[11]	TAN L T, ZHU Y X, FAN T, PENG C, WANG J R, SUN L, CHEN C Y. OsZIP 7 functions in xylem loading in roots and inter-vascular transfer in nodes to deliver Zn/Cd to grain in rice. Biochemical and Biophysical Research Communications, 2019, 512(1): 112-118. doi: 10.1016/j.bbrc.2019.03.024
[12]	李虹呈, 王倩倩, 贾润语, 辜娇峰, 周航, 杨文弢, 张平, 彭佩钦, 廖柏寒. 外源锌对水稻各部位镉吸收与累积的拮抗效应. 环境科学学报, 2018, 38(12): 4854-4863.
	LI H C, WANG Q Q, JIA R Y, GU J F, ZHOU H, YANG W T, ZHANG P, PENG P Q, LIAO B H. Antagonistic effects of exogenous zinc on uptake and accumulation of cadmium in various rice organs. Acta Scientiae Circumstantiae, 2018, 38(12): 4854-4863. (in Chinese)
[13]	MUHAMMAD Q, SHAHID H, ZED R. Zinc fertilisation increases grain zinc and reduces grain lead and cadmium concentrations more in zinc-biofortified than standard wheat cultivar. Science of the Total Environment, 2017, 605-606: 454-460.
[14]	董如茵, 徐应明, 王林, 赵明阳, 梁学峰, 孙约兵. 土施和喷施锌肥对镉低积累油菜吸收镉的影响. 环境科学学报, 2015, 35(8): 2589-2596.
	DONG R Y, XU Y M, WANG L, ZHAO M Y, LIANG X F, SUN Y B. Effects of soil application and foliar spray of zinc fertilizer on cadmium uptake in a pakchoi cultivar with low cadmium accumulation. Acta Scientiae Circumstantiae, 2015, 35(8): 2589-2596. (in Chinese)
[15]	韩潇潇, 任兴华, 王培培, 黄永春, 张长波, 刘仲齐. 叶面喷施锌离子对水稻各器官镉积累特性的影响. 农业环境科学学报, 2019, 38(8): 1809-1817.
	HAN X X, REN X H, WANG P P, HUANG Y C, ZHANG C B, LIU Z Q. Effects of foliar application with zinc on the characteristics of cadmium accumulation in organs of rice plants. Journal of Agro- Environment Science, 2019, 38(8): 1809-1817. (in Chinese)
[16]	HUANG G X, DING C F, MA Y B, WANG Y R, ZHOU Z G, ZHENG S A, WANG X X. Rice (Oryza sativa L.) seedlings enriched with zinc or manganese: Their impacts on cadmium accumulation and expression of related genes. Pedosphere, 2021, 31(6): 849-858. doi: 10.1016/S1002-0160(20)60047-9
[17]	ZHOU Q, SHAO G S, ZHANG Y X, DONG Q, WANG H, CHENG S H, CAO L Y, SHEN X H. The difference of cadmium accumulation between the indica and japonica subspecies and the mechanism of it. Plant Growth Regulation, 2017, 81(3): 523-532. doi: 10.1007/s10725-016-0229-0
[18]	HUANG G X, DING C F, Li Y S, ZHANG T L, WANG X X. Selenium enhances iron plaque formation by elevating the radial oxygen loss of roots to reduce cadmium accumulation in rice (Oryza sativa L.). Journal of Hazardous Materials, 2020, 398: 122860. doi: 10.1016/j.jhazmat.2020.122860
[19]	HOUBA V J G, TEMMINGHOFF E J M, GAIKHORST G A, VAN VARK W. Soil analysis procedures using 0.01 M calcium chloride as extraction reagent. Communications in Soil Science and Plant Analysis, 2000, 31(9/10): 1299-1396. doi: 10.1080/00103620009370514
[20]	HUANG G X, DING C F, HU Z Y, CUI C H, ZHANG T L, WANG X X. Topdressing iron fertilizer coupled with pre-immobilization in acidic paddy fields reduced cadmium uptake by rice (Oryza sativa L.). Science of the Total Environment, 2018, 636: 1040-1047. doi: 10.1016/j.scitotenv.2018.04.369
[21]	鲁如坤. 土壤农业化学分析方法. 北京: 中国农业科技出版社, 2000.
	LU R K. Analysis Methods of Soil Agro-Chemistry. Beijing: China Agricultural Science and Technology Press, 2000. (in Chinese)
[22]	王北洪, 马智宏, 付伟利. 密封高压消解罐消解-原子吸收光谱法测定土壤重金属. 农业工程学报, 2008, 24(S2): 255-259.
	WANG B H, MA Z H, FU W L. Determination of heavy metal in soil by high pressure sealed vessels assisted digestion-atomic absorption spectrometry. Transactions of the Chinese Society of Agricultural Engineering, 2008, 24(S2): 255-259. (in Chinese)
[23]	HUANG S, YAMAJI N, MA J F. Zinc transport in rice: How to balance optimal plant requirements and human nutrition. Journal of Experimental Botany, 2022, 73(6): 1800-1808. doi: 10.1093/jxb/erab478
[24]	张标金, 罗林广, 魏益华, 张祥喜. 不同基因型水稻锌积累动态过程的比较. 江西农业学报, 2015, 27(9): 6-10.
	ZHANG B J, LUO L G, WEI Y H, ZHANG X X. Comparative study on dynamic process of zinc accumulation in different genotypes of rice. Acta Agriculturae Jiangxi, 2015, 27(9): 6-10. (in Chinese)
[25]	薛欣月, 于雪然, 刘晓刚, 马嘉欣, 田蕾, 李培富. 水稻锌吸收、转运、累积机理研究进展. 生物技术通报, 2022, 38(4): 29-43.
	XUE X Y, YU X R, LIU X G, MA J X, TIAN L, LI P F. Research progress on absorption, transportation and accumulation mechanism of zinc in rice. Biotechnology Bulletin, 2022, 38(4): 29-43. (in Chinese)
[26]	YANG M, LI Y T, LIU Z H, TIAN J J, LIANG L M, QIU Y, WANG G Y, DU Q Q, CHENG D, CAI H M, SHI L, XU F S, LIAN X M. A high activity zinc transporter OsZIP9 mediates zinc uptake in rice. The Plant Journal, 2020, 103(5): 1695-1709. doi: 10.1111/tpj.14855
[27]	汪鹏, 王静, 陈宏坪, 周东美, 赵方杰. 我国稻田系统镉污染风险与阻控. 农业环境科学学报, 2018, 37(7): 1409-1417.
	WANG P, WANG J, CHEN H P, ZHOU D M, ZHAO F J. Cadmium risk and mitigation in paddy systems in China. Journal of Agro- Environment Science, 2018, 37(7): 1409-1417. (in Chinese)
[28]	CHEN Z, TANG Y T, YAO A J, GAO J, WU Z H, PENG Z R, WANG S Z, XIAO S, BAKER A J M, QIU R L. Mitigation of Cd accumulation in paddy rice (Oryza sativa L.) by Fe fertilization. Environmental Pollution, 2017, 231: 549-559. doi: 10.1016/j.envpol.2017.08.055
[29]	SASAKI A, YAMAJI N, YOKOSHO K, MA J F. Nramp5 is a major transporter responsible for manganese and cadmium uptake in rice. The Plant Cell, 2012, 24(5): 2155-2167. doi: 10.1105/tpc.112.096925
[30]	UENO D, YAMAJI N, KONO I, HUANG C F, ANDO T, YANO M, MA J F. Gene limiting cadmium accumulation in rice. Proceedings of the National Academy of Sciences of the United States of America, 2010, 107(38): 16500-16505.
[31]	TAKAHASHI R, ISHIMARU Y, SHIMO H, OGO Y, SENOURA T, NISHIZAWA N K, NAKANISHI H. The OsHMA2 transporter is involved in root-to-shoot translocation of Zn and Cd in rice. Plant Cell and Environment, 2012, 35(11): 1948-1957. doi: 10.1111/j.1365-3040.2012.02527.x
[32]	ALI S, MFARREJ M F B, HUSSAIN A, AKRAM N A, RIZWAN M, WANG X K, MAQBOOL A, NAFEES M, ALI B. Zinc fortification and alleviation of cadmium stress by application of lysine chelated zinc on different varieties of wheat and rice in cadmium stressed soil. Chemosphere, 2022, 295: 133829. doi: 10.1016/j.chemosphere.2022.133829
[33]	WANG Y F, SU Y, LU S G. Predicting accumulation of Cd in rice (Oryza sativa L.) and soil threshold concentration of Cd for rice safe production. Science of the Total Environment, 2020, 738: 139805. doi: 10.1016/j.scitotenv.2020.139805
[34]	YANG Y, LI Y L, WANG M E, CHEN W P, DAI Y T. Limestone dosage response of cadmium phytoavailability minimization in rice: A trade-off relationship between soil pH and amorphous manganese content. Journal of Hazardous Materials, 2021, 403: 123664. doi: 10.1016/j.jhazmat.2020.123664
[35]	张上都, 罗正良, 伍祥, 石邦志, 彭菊, 蒙秀菲, 蒋慧丹, 周乐良. 不同温度下水稻各生育期对镉累积效应研究. 种子, 2021, 40(12): 39-44.
	ZHANG S D, LUO Z L, WU X, SHI B Z, PENG J, MENG X F, JIANG H D, ZHOU L L. Effects of different temperature on cadmium accumulation in rice at different growth stages. Seed, 2021, 40(12): 39-44. (in Chinese)
[36]	罗玲, 方宝华, 刘洋, 余锋, 李强, 张玉烛. 不同生育时期温度处理对水稻镉积累特性的影响. 杂交水稻, 2020, 35(2): 52-59.
	LUO L, FANG B H, LIU Y, YU F, LI Q, ZHANG Y Z. Effects of temperature treatment in different growth stages on cadmium accumulation characteristics in rice. Hybrid Rice, 2020, 35(2): 52-59. (in Chinese)
[37]	何洋, 刘洋, 方宝华, 李超, 杨坚, 滕振宁, 何小娥, 张玉烛. 温度对不同水稻品种糙米镉(Cd)含量的影响. 中国稻米, 2016, 22(2): 31-35.
	HE Y, LIU Y, FANG B H, LI C, YANG J, TENG Z N, HE X E, ZHANG Y Z. Effect of temperature on cadmium content of brown rice in different cultivars. China Rice, 2016, 22(2): 31-35. (in Chinese)