壳聚糖为递送载体的鸭肠炎病毒gC基因疫苗诱导Balb/c小鼠

doi:10.3864/j.issn.0578-1752.2011.16.019

中国农业科学 ›› 2011, Vol. 44 ›› Issue (16): 3454-3462.doi: 10.3864/j.issn.0578-1752.2011.16.019

壳聚糖为递送载体的鸭肠炎病毒gC基因疫苗诱导Balb/c小鼠

蒋金凤, 程安春, 汪铭书, 路立婷, 朱德康, 贾仁勇, 陈孝跃

1. 四川农业大学预防兽医研究所
2. 四川农业大学动物医学院禽病防治研究中心
3. 四川农业大学动物医学院动物疫病与人类健康四川省重点实验室

收稿日期:2010-11-27 修回日期:2011-04-11 出版日期:2011-08-15 发布日期:2011-04-25
通讯作者: 通信作者程安春和汪铭书，Tel：0835-2885774/2882754(Fax)；E-mail：chenganchun@vip.163.com
作者简介:蒋金凤，E-mail：jjf403@yahoo.com.cn
基金资助:
教育部“长江学者和创新团队发展计划”创新团队项目（IRT0848）和现代农业产业技术体系建设专项资金（CARS-43-8）、“973”计划（2011CB111606）和国家自然科学基金（31072157）

The Immunoreaction in Balb/c Mice Immunized with Duck Enteritis Virus gC Genetic Vaccine with Chitosan as Deliver Carrier

JIANG Jin-Feng, CHENG An-Chun, WANG Ming-Shu, LU Li-Ting, ZHU De-Kang, JIA Ren-Yong, CHEN Xiao-Yue

1. 四川农业大学预防兽医研究所
2. 四川农业大学动物医学院禽病防治研究中心
3. 四川农业大学动物医学院动物疫病与人类健康四川省重点实验室

Received:2010-11-27 Revised:2011-04-11 Online:2011-08-15 Published:2011-04-25

摘要/Abstract

摘要： 【目的】探讨壳聚糖对鸭肠炎病毒（duck enteritis virus, DEV）gC基因疫苗免疫反应的影响。【方法】应用壳聚糖作为DEV gC基因疫苗（pcDNA-DEV-gC）递送载体，采用肌肉注射和口服单次免疫Balb/c小鼠50µg剂量基因疫苗，并以肌注不同剂量裸质粒组、弱毒组和生理盐水组作为对照，分别检测不同疫苗免疫后的细胞、体液和黏膜免疫水平。【结果】肌注壳聚糖疫苗组诱导小鼠产生较肌注裸质粒50µg组和口服壳聚糖疫苗组高的细胞和体液免疫反应，且产生与弱毒疫苗相似的细胞免疫应答，但弱毒疫苗诱导体液免疫的能力更强，仅口服壳聚糖疫苗组产生粘膜免疫。【结论】上述研究结果表明壳聚糖具有一定的佐剂效应，为获得更有效的DEV gC基因疫苗提供了新策略，但该疫苗的推广应用还有待于对影响疫苗免疫效果的各因素进行进一步优化。

关键词: 壳聚糖, DEV gC基因, 基因疫苗, 细胞免疫, 体液免疫, 粘膜免疫

Abstract: 【Objective】 The objective of the experiment is to investigate the influence of chitosan on the immunoreaction induced duck enteritis virus gC genetic vaccine. 【Method】 Balb/c mice were immunized with 50µg per dose of DEV gC genetic vaccine with chitosan as deliver carrier by a single intramuscular injection and oral. Naked plasmid groups of different immunizing doses by intramscular injection, live attenuated vaccine group and physiological saline group were applied as control groups. Cellular immunity, humoral immunity and mucosal immunity were detected, respectively. 【Result】 Compared with DEV gC genetic vaccine with chitosan as deliver carrier by oral and 50 µg of naked plasmid group by intramuscular injection, DEV gC genetic vaccine with chitosan as deliver carrier by intramuscular injection induced stronger cellular and humoral immunity. This vaccine induced similar cellular immune response compared with live attenuated vaccine that induced stronger humoral immune responses. Mucosal immunity was induced only by oral DEV gC genetic vaccine with chitosan as deliver carrier. 【Conclusion】 Chitosan had certain adjuvant’s ability. It has provided a new strategy to obstain more effective DEV gC genetic vaccine. Many factors affecting the immunity efficiency of vaccine need to be optimiazed further for application of this vaccine generally .

Key words: chitosan, DEV gC gene, genetic vaccine, cellular immunity, humoral immunity, mucosal immunity

蒋金凤, 程安春, 汪铭书, 路立婷, 朱德康, 贾仁勇, 陈孝跃. 壳聚糖为递送载体的鸭肠炎病毒gC基因疫苗诱导Balb/c小鼠[J]. 中国农业科学, 2011, 44(16): 3454-3462.

JIANG Jin-Feng, CHENG An-Chun, WANG Ming-Shu, LU Li-Ting, ZHU De-Kang, JIA Ren-Yong, CHEN Xiao-Yue. The Immunoreaction in Balb/c Mice Immunized with Duck Enteritis Virus gC Genetic Vaccine with Chitosan as Deliver Carrier[J]. Scientia Agricultura Sinica, 2011, 44(16): 3454-3462.

0
/ 收藏文章 0 / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2011.16.019

https://www.chinaagrisci.com/CN/Y2011/V44/I16/3454

参考文献

[1]Shawky S, Sandhu T. Inactivated vaccine for protection against duck virus enteritis. Avian Diseases, 1997, 41: 461-468.

[2]程安春, 汪铭书, 崔恒敏. 鸭瘟鸭病毒性肝炎二联弱毒疫苗的研究1.最佳配比的筛选, 安全性及免疫效力. 畜牧兽医学报, 1996, 26: 466-474.

Cheng A C, Wang M S, Cui H M, Siudy on bivalent attenuated vaccines against DP and DVH 1. the most appropriate proportion, safety and immune efficaycy test. Acta Veterinaria et Zootechnica Sinica, 1996, 26(5): 466-474. (in Chinese)

[3]Islam M, Samad M, Rahman M, Hossain M, Akter S. Assessment of immunologic responses in khaki cambell ducks vaccinated against duck plague. Trends in Pharmacol Sciences, 2005, 4: 36-38.

[4]Shams H. Recent developments in veterinary vaccinology. Veterinary Journal, 2005, 170: 289-299.

[5]刁有祥, 吕桂霞, 郑福英, 王刚. 一种新型鸭瘟病原的分离鉴定及其特征. 中国兽医学报, 2006, 26(2): 136-139.

Diao Y X, Lv G X, Zheng H Y, Wang G. Isolation and identification and characteristics of the pathogen of a new duck plague. Chinese Veterinary Science, 2006, 26(2): 136-139. (in Chinese)

[6]Spear P G. Herpes simplex virus: receptors and ligands for cell entry. Cell Microbiology, 2004, 6: 401-410.

[7]Kaashoek M J, Rijsewijk F A, Ruuls R C, Keil G M, Thiry E, Pastoret P P, Van Oirschot J T. Virulence, immunogenicity and reactivation of bovine herpesvirus 1 mutants with a deletion in the gC, gG, gI, gE, or in both the gI and gE gene. Vaccine, 1998, 16: 802-809.

[8]Chang Y J, Jiang M, Lubinski J M, King R D, Friedman H M. Implications for herpes simplex virus vaccine strategies based on antibodies produced to herpes simplex virus type 1 glycoprotein gC immune evasion domains. Vaccine, 2005, 23: 4658-4665.

[9]Ober B T, Teufel B, Wiesmuller K H, Jung G, Pfaff E, Saalmuller A, Rziha H J. The porcine humoral immune response against pseudorabies virus specifically targets attachment sites on glycoprotein gC. Journal of Virology, 2000, 74: 1752-1760.

[10]Ober B T, Summerfield A, Mattlinger C, Wiesmuller K H, Jung G, Pfaff E, Saalmuller A, Rziha H J. Vaccine-induced, pseudorabies virus-specific, extrathymic CD4+CD8+ memory T-helper cells in swine. Journal of Virology, 1998, 72: 4866-4873.

[11]Gupta P K, Saini M, Gupta L K, Rao V D, Bandyopadhyay S K, Butchaiah G, Garg G K, Garg S K. Induction of immune responses in cattle with a DNA vaccine encoding glycoprotein C of bovine herpesvirus-1. Veterinaryt Microbiology, 2001, 78: 293-305.

[12]Gogev S, Vanderheijden N, Lemaire M, Schynts F, D'Offay J, Deprez I, Adam M, Eloit M, Thiry E. Induction of protective immunity to bovine herpesvirus type 1 in cattle by intranasal administration of replication-defective human adenovirus type 5 expressing glycoprotein gC or gD. Vaccine, 2002, 20: 1451-1465.

[13]Gerdts V, Jons A, Makoschey B, Visser N, Mettenleiter T C. Protection of pigs against Aujeszky's disease by DNA vaccination. Journal of General Virology, 1997, 78 ( Pt 9): 2139-2146.

[14]Jr.Dubensky T W, Liu M A, Ulmer J B. Delivery systems for gene-based vaccines. Molecular Medicine, 2000, 6: 723-732.

[15]Dupuis M, Denis-Mize K, Woo C, Goldbeck C, Selby M J, Chen M, Otten G R, Ulmer J B, Donnelly J J, Ott G, McDonald D M. Distribution of DNA vaccines determines their immunogenicity after intramuscular injection in mice. Journal of Immunology, 2000, 165: 2850-2858.

[16]Guliyeva Ü, ner F, Özsoy S, Haziroglu R. Chitosan microparticles containing plasmid DNA as potential oral gene delivery system. European Journal of Pharmaceutics and Biopharmaceutics, 2006, 62: 17-25.

[17]Bowman K, Sarkar R, Raut S, Leong K. Gene transfer to hemophilia A mice via oral delivery of FVIII-chitosan nanoparticles. Journal of Controlled Release, 2008, 132: 252-259.

[18]Koping-Hoggard M, Tubulekas I, Guan H, Edwards K, Nilsson M, Varum K M, Artursson P. Chitosan as a nonviral gene delivery system. Structure-property relationships and characteristics compared with polyethylenimine in vitro and after lung administration in vivo. Gene Therapy, 2001, 8: 1108-1121.

[19]Mao H, Roy K, Troung-Le V, Janes K, Lin K, Wang Y, August J, Leong K. Chitosan-DNA nanoparticles as gene carriers: synthesis, characterization and transfection efficiency. Journal of Control Release, 2001, 70: 399-421.

[20]Elson C O, Ealding W, Lefkowitz J. A lavage technique allowing repeated measurement of IgA antibody in mouse intestinal secretions. Journal of Immunol Methods, 1984, 67: 101-108.

[21]柳忠辉, 吕昌龙. 免疫学常用实验技术. 北京: 科学出版社, 2002.

Liu Z H, Lv C L. Used Immunological Test Technology. Beijing: Science Press, 2002. (in Chinese)

[22]Ulmer J B, Wahren B, Liu M A. Gene-based vaccines: recent technical and clinical advances. Trends Molecular Medicine, 2006, 12: 216-222.

[23]Chalmers W S. Overview of new vaccines and technologies. Veterinaryt Microbiology, 2006, 117: 25-31.

[24]Wolff J, Malone R, Williams P, Chong W, Acsadi G, Jani A, Felgner P. Direct gene transfer into mouse muscle in vivo. Science, 1990, 247: 1465.

[25]Greenland J R, Letvin N L. Chemical adjuvants for plasmid DNA vaccines. Vaccine, 2007, 25: 3731-3741.

[26]Khatri K, Goyal A K, Gupta P N, Mishra N, Vyas S P. Plasmid DNA loaded chitosan nanoparticles for nasal mucosal immunization against hepatitis B. International Journal of Pharmaceutics, 2008, 354: 235-241.

[27]Illum L, Jabbal-Gill I, Hinchcliffe M, Fisher A N, Davis S S. Chitosan as a novel nasal delivery system for vaccines. Advanced Drug Delivery Reviews, 2001, 51: 81-96.

[28]Babiuk S, Mookherjee N, Pontarollo R, Griebel P, van Drunen Littel-van den Hurk S, Hecker R, Babiuk L. TLR9-/- and TLR9+/+ mice display similar immune responses to a DNA vaccine. Immunology, 2004, 113: 114-120.

[29]李琦涵, 姜莉. 病毒感染的分子生物学. 北京: 化学工业出版社, 2004.

Li Q H, Jiang L. The Molecular Biology of Viral Infection. Beijing: Chemical Industry Press, 2004. (in Chinese)

Metrics

Viewed

Full text

178

HTML			PDF

Just accepted	Online first	Issue	Just accepted	Online first	Issue
0	0	0	0	0	178

From	Others	local

Times	83	95
Rate	47%	53%

Abstract

193

Just accepted	Online first	Issue

0	0	193

From	Others	local

Times	184	9
Rate	95%	5%

Cited

Web of Science	Crossref	ScienceDirect	Search for Citations in Google Scholar >>


This page requires you have already subscribed to WoS.

Shared

壳聚糖为递送载体的鸭肠炎病毒gC基因疫苗诱导Balb/c小鼠

The Immunoreaction in Balb/c Mice Immunized with Duck Enteritis Virus gC Genetic Vaccine with Chitosan as Deliver Carrier

PDF

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	付中民,陈华枝,刘思亚,祝智威,范小雪,范元婵,万洁琦,张璐,熊翠玲,徐国钧,陈大福,郭睿. 意大利蜜蜂响应东方蜜蜂微孢子虫胁迫的免疫应答[J]. 中国农业科学, 2019, 52(17): 3069-3082.
[2]	梁迪，杨曦，郭玉蓉. 不同浓度苹果多酚对壳聚糖复合膜液理化性质的影响[J]. 中国农业科学, 2018, 51(14): 2799-2813.
[3]	王宝刚, 侯玉茹, 李文生, 杨军军, 石磊, 杨媛, 冯晓元. 壳聚糖处理对‘早红考密斯’梨虎皮病抑制和贮藏品质的影响[J]. 中国农业科学, 2013, 46(16): 3424-3431.
[4]	马彦霞, 郁继华, 张国斌, 曹刚. 壳聚糖对水分胁迫下辣椒幼苗氧化损伤的保护作用[J]. 中国农业科学, 2012, 45(10): 1964-1971.
[5]	朱德康,黎敏,车茜,程安春,汪铭书,陈孝跃 . 小鹅瘟病毒VP3真核表达质粒与弱毒疫苗诱导鹅体免疫应答的比较[J]. 中国农业科学, 2011, 44(3): 595-603 .
[6]	朱叶萌,谢正军,李云涛,韩新燕 . 壳聚糖铜对断奶仔猪生产性能、肠道菌群及黏膜形态的影响 [J]. 中国农业科学, 2011, 44(2): 387-394 .
[7]	沈福晓, 蒋金凤, 程安春, 汪铭书, 路立婷, 贾仁勇, 朱德康, 陈孝跃, 孙涛. 壳聚糖/pcDNA-DPV-gC基因在雏鸭体内的抗原表达和分布[J]. 中国农业科学, 2011, 44(18): 3909-3917.
[8]	张燕, 伏红伟, 王爽, 梁国华, 杨益众. 利用RNAi技术防治水稻条纹叶枯病[J]. 中国农业科学, 2011, 44(13): 2683-2691 .
[9]	夏庆祥;张德庆;吴家强;牛星;王小龙;牛钟相; . 猪C3d分子佐剂PRRSV GP5基因核酸疫苗的构建及免疫效果的研究[J]. 中国农业科学, 2011, 44(12): 2582-2588 .
[10]	邓雨艳,明建,张昭其,曾凯芳 . 壳聚糖诱导脐橙果实抗病性、水杨酸及活性氧代谢变化 [J]. 中国农业科学, 2010, 43(4): 812-820 .
[11]	罗自生,张莉 . 壳聚糖/纳米SiOx复合物涂膜对鲜切竹笋品质和生理的影响[J]. 中国农业科学, 2010, 43(22): 4694-4700 .
[12]	王全溪,吴宝成,李国平,黄一帆 . 番鸭呼肠孤病毒感染对体液免疫功能的影响[J]. 中国农业科学, 2010, 43(2): 424-429 .
[13]	柳风祥,崔治中,郭慧君 . 复方中药提取物对REV诱发的免疫抑制鸡的免疫增强作用[J]. 中国农业科学, 2009, 42(6): 2164-2171 .
[14]	. 壳聚糖的抗菌性及其对果实病害的防治研究进展[J]. 中国农业科学, 2009, 42(2): 626-635 .
[15]	杨玉荣,佘锐萍,梁宏德 . 雏鸡服用AvBD13后体液免疫的动态变化[J]. 中国农业科学, 2009, 42(10): 3679-3684 .