关键词: RNAi技术, 基因疫苗, 水稻条纹病毒, 浸种与喷雾, 控制效果

Abstract: 【Objective】The objective of this study is to control the incidence and prevalence of rice stripe disease, change the situation of that there is no specific pesticide to inhibit the occurrence of rice stripe disease once rice is infected with RSV. 【Method】Seed soaking and spraying technologies were used to make biological vaccines absorbed by rice plants. Both the detection of RSV-carrying rate and vaccine residue in rice plant and field survey on occurrence rate of rice stripe disease were conducted to determine vaccine control efficiency to the disease. 【Result】Addition of 1.0‰ B penetrant in the vaccine was the optimal concentration for seed soaking and 5.0‰ A penetrant was the optimal concentration for spraying. The detection rate of vaccine was consistent with the degeneration of RSV. The residue time of vaccine in rice plant was more than 30 d after seed soaking and 10-20 d after spraying. The control effects of two methods both exceeded 70%.【Conclusion】Biological vaccine could be a new biological pesticide for the control of rice stripe disease.

Key words: RNAi technology, genic vaccine, RSV, soaking and spraying, control effect