宠物源大肠杆菌质粒介导喹诺酮类耐药基因流行性检测

doi:10.3864/j.issn.0578-1752.2010.16.022

中国农业科学 ›› 2010, Vol. 43 ›› Issue (16): 3447-3454 .doi: 10.3864/j.issn.0578-1752.2010.16.022

宠物源大肠杆菌质粒介导喹诺酮类耐药基因流行性检测

朱恒乾,廖晓萍,陈朝喜,王秀梅,孙坚,孙迎,李亮,张美君,刘雅红

(华南农业大学兽医学院/广东省兽药研制与安全评价重点实验室)

收稿日期:2009-12-25 修回日期:2010-04-01 出版日期:2010-08-15 发布日期:2010-08-15
通讯作者: 刘雅红

Detection of Plasmid-Mediated Quinolone Resistance in Clinical Isolates of Escherichia coli from Pet Animals

ZHU Heng-qian, LIAO Xiao-ping, CHEN Chao-xi, WANG Xiu-mei, SUN Jian, SUN ying,LI Liang, ZHANG Mei-jun, LIU Ya-hong

(华南农业大学兽医学院/广东省兽药研制与安全评价重点实验室)

Received:2009-12-25 Revised:2010-04-01 Online:2010-08-15 Published:2010-08-15
Contact: LIU Ya-hong

摘要/Abstract

摘要：

【目的】对广州分离得到的宠物源(主要是宠物猫、狗)大肠杆菌进行质粒介导喹诺酮类耐药PMQR基因qnr、qepA和aac(6′)-Ib-cr基因的流行性检测。【方法】采用琼脂平板稀释法对164株临床分离的宠物源大肠杆菌进行15种抗菌药物的最小抑菌浓度测定。通过PCR检测qnr、qepA和aac(6′)-Ib-cr基因。建立大肠杆菌基因指纹图谱并对指纹图谱进行分析。【结果】164株宠物源大肠杆菌对12种兽医临床常用抗生素耐药率较高,且表现为多重耐药(5-14 耐)。在164株宠物源大肠杆菌中检测到3个菌株同时携带qnrA、qnrB和qnrS基因,其中2株也携带aac(6′)-Ib-cr基因。阳性菌株均能扩增出指纹图谱,具有两个以上PMQR基因的菌株多分布于B型和C型。【结论】广州市宠物源大肠杆菌对临床常用抗菌药物耐药较为严重,检测结果显示喹诺酮类耐药基因在本市宠物医学临床上传播广泛,PMQR基因的产生能力与其基因型有密切的关系。

关键词: 宠物, 大肠埃希氏菌, 质粒介导, 喹诺酮, 耐药基因

Abstract:

【Objective】 The plasmid-mediated quinolone resistance PMQR genes of qnr, qepA and aac (6′)-Ib-cr in clinical isolates of Escherichia coli from pet animals (dogs and cats) in Guangzhou city were detected. 【Method】 Susceptibility of 164 strains to 15 antimicrobial agents were determined by agar disc dilution method. All of the 164 strains were screened for the qnr, qepA, aac (6′)-Ib-cr genes by PCR. Genetic fingerprints of the clinical isolates were established and analyzed. 【Results】 The isolates showed a high rate of resistance to antimicrobial agents widely used in veterinary clinical medicine and most of the them showed multiple-resistance(5 to 14 drugs). Three of these 164 specimens were detected containing qnrA, qnrB and qnrS genes, and two of which also posses aac(6′)-Ib-cr gene. Fingerprints could be amplifled from all the positive strains, and strains with more than two PMQR genes were distributed mainly in the B and C gene types. 【Conclusion】 Escherichia coli isolated from pet animals in Guangzhou city showed a high level multiple-resistance to widely used drugs. The result of the research showed a more widespread dissemination of resistance to quinolones in pet animal veterinary clinic of Guangzhou city, and the ability to generate PMQR gene of isolates is closely to their gene-type.

Key words: pet animals, Escherichia coli, plasmid mediate, quinolones, resistance gene

朱恒乾,廖晓萍,陈朝喜,王秀梅,孙坚,孙迎,李亮,张美君,刘雅红 . 宠物源大肠杆菌质粒介导喹诺酮类耐药基因流行性检测
[J]. 中国农业科学, 2010, 43(16): 3447-3454 .

ZHU Heng-qian,LIAO Xiao-ping,CHEN Chao-xi,WANG Xiu-mei,SUN Jian,SUN ying,LI Liang,ZHANG Mei-jun,LIU Ya-hong. Detection of Plasmid-Mediated Quinolone Resistance in Clinical Isolates of Escherichia coli from Pet Animals
[J]. Scientia Agricultura Sinica, 2010, 43(16): 3447-3454 .

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宠物源大肠杆菌质粒介导喹诺酮类耐药基因流行性检测

Detection of Plasmid-Mediated Quinolone Resistance in Clinical Isolates of Escherichia coli from Pet Animals

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