动物源大肠杆菌PMQR基因流行性检测

doi:10.3864/j.issn.0578-1752.2012.10.018

摘要/Abstract

摘要： 【目的】检测分离自广东省散养型养殖场的动物源大肠杆菌中oqxAB基因及其它质粒介导喹诺酮类耐药基因(PMQR)的流行情况。【方法】采用PCR方法检测oqxA、oqxB、qnr、qepA和aac（6′）-Ib-cr基因；琼脂平板稀释法对PMQR阳性菌株进行18种抗菌药物的最小抑菌浓度测定。【结果】qnrB 、qnrS、aac（6′）-Ib-cr、oqxA、oqxB的检出率依次为10.49%、18.88%、31.47%、44.8%和48.9%，但未检测到qnrA、qnrC、qnrD和qepA。oqxA与oqxB的检出率较高，且oqxA与oqxB常常同时存在。多数菌株同时携带两个或两个以上的PMQR基因。PMQR阳性菌对18种兽医临床常用抗生素耐药率较高。【结论】PMQR基因在广东省兽医临床传播广泛，广东省大肠杆菌对临床常用抗菌药物耐药较为严重，药敏谱呈多样化，多重耐药株比例较高。

关键词: oqxAB基因, 大肠埃希氏菌, 质粒介导, 喹诺酮, 耐药基因

Abstract: 【Objective】The objective of the study is to investigate the prevalence of oqxAB gene and other three types of plasmid-mediated quinolone resistance ( PMQR) gene in Escherichia coli isolated from free-range farms in Guangdong province.【Method】All the strains were screened for the oqxA, oqxB, qnr, qepA, aac (6′)-Ib-cr genes by PCR. The susceptibility of PMQR- positive strains to 18 antimicrobial agents were determined by agar dilution method.【Results】The positive rates of qnrB, qnrS, aac（6′）-Ib-cr，oqxA，oqxB genes were 10.49%, 18.88%, 31.47%, 44.8%, and 48.9% respectively. No qnrA, qnrC, qnrD and qepA genes were detected in any of the isolates. oqxA and oqxB have a high positive rate, and the two genes always exsist in the same isolates. PMQR gene always shows a coexstence of two or more kinds of the plasmid genes in the same isolates. PMQR positive isolates show a high rate of resistance to 18 antimicrobial agents which was widely used in veterinary clinical medicine. 【Conclusion】PMQR gene is very common in veterinary clinic. E. coli isolated from animals in Guangdong province shows a high level resistance to widely used drugs. The antibiotic sensitivity spectrum is variegated. The proportion of multi-drug-resistant strains is high.

Key words: oqxAB gene , Escherichia coli, plasmid mediate, quinolones, resistance gene

庄娜, 陈雪影, 岳磊, 廖晓萍, 刘雅红. 动物源大肠杆菌PMQR基因流行性检测[J]. 中国农业科学, 2012, 45(10): 2052-2057.

ZHUANG Na, CHEN Xue-Ying, YUE Lei, LIAO Xiao-Ping, LIU Ya-Hong. Detection of PMQR Gene in Escherichia coli Isolated from Animals[J]. Scientia Agricultura Sinica, 2012, 45(10): 2052-2057.

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