关键词: 肌动蛋白解聚因子, 转基因棉花, 基因组, 纤维品质

Abstract:

【Objective】 To detect the effects of suppressing actin-depolymerizing factor gene of G. hirsutum L. (GhADF1), antisense GhADF1 gene was introduced into upland cotton. 【Method】 The ORF of GhADF1 was inserted into plant expression vector pPZP111 in the anti-sense orientation. The recombinant plasmid was introduced into cotton Coker312 (G. hirsutum L.) by the method of agrobacterium-mediated transformation. 【Result】 PCR and Southern blot showed that the antisense GhADF1 gene was integrated into the cotton genome and could be inherited stably. The fiber quality traits analysis indicated that the fiber length and strength of 3 pure transgenic lines were significantly improved compared with that of Coker312. 【Conclusion】 Transformation of antisense GhADF1 gene could improve fiber strength and cotton fiber quality.

Key words: actin-depolymerizing factor gene of G. hirsutum L. (GhADF1), transgenic cotton, genome, fiber quality analysis