关键词: 水稻条纹叶枯病, 水稻品种, 抗性特征, 遗传分析, 基因定位

Abstract:

【Objective】 The objective of this experiment was to analyze the resistance mechanism and resistance genetic model to rice stripe disease in Zhendao 88, and to screen the SSR marker related to the gene of rice stripe disease resistance in Zhendao 88. 【Method】The resistance to rice stripe virus (RSV) and the vector, small brown planthopper (SBPH) (Laodelphax striatellus Fallèn) was analyzed in Zhendao 88 by using the method of tiller inoculation, inoculation at the seeding stage, non-preference test and antibiosis test. The inheritance of rice stripe disease resistance in F2 lines and F2:3 lines from the cross Zhendao 88/ Wuyujing No. 3 was studied by using tiller inoculation and inoculation at the seeding stage, respectively. The resistance gene was mapped based on SSR analysis.【Result】 Zhendao 88 was highly resistant to rice stripe virus and weakly resistant to vector. Genetic analysis indicated that the resistance to rice stripe disease in Zhendao 88 was inherited as a single dominant trait. The rice stripe disease resistance gene in Zhendao 88 was located on the chromosome 11, with the distance of 4.7cM from a SSR marker RM229. 【Conclusion】 The rice stripe disease resistance in Zhendao 88 was mainly derived from the resistance to virus and controlled by a single major gene. The breeding process for rice stripe disease resistance should be accelerated by using Zhendao 88 as a resistance parent.

Key words: rice stripe disease, rice cultivar, resistance mechanism, genetic analyze, gene mapping