关键词: H-FABP基因型, 湖北白猪, 杂交后代, 相关性状

Abstract: The genetic variation of 5，-Up stream Region and the Second Intron of heart fatty acid-binding protein(H-FABP)gene in 282 pigs including Hubei white pig and its crossbred were detected by PCR-RFLP with HinfⅠ,HaeⅢ and MspⅠ.Meanwhile,effects of different PCR-RFLP genotype of H-FABP gene of crossbreed on related traits were analyzed.The results showed as follows:(1) Hubei white pig had no polymorphism at HinfⅠ-RFLP site which had only HH Genotype, but it had polymorphism at HaeⅢ-RFLP and MspⅠ-RFLP site.Crossbred F1 and F2 had polymorphism at these three site;(2) Hubei white pig had low polymorphism at HinfⅠ-RFLP and HaeⅢ-RFLP site （PIC＜0.25）while it had mediate polymorphism（0.25＜PIC＜0.5）at MspⅠ-RFLP site., Crossbred F1 and F2 had mediate polymorphism at these three site.（3）The effect of different H-FABP genotype on IMF content tended to be HH>Hh>hh, dd＞Dd＞DD，aa＞Aa＞AA.IMF content of dd genotype was significantly higher（P＜0.05）than that of DD and Dd genotype at the HaeⅢ-RFLP site. IMF content of aa and Aa genotype was significantly higher （P＜0.01）than that of DD and AA genotype at the MspⅠ-RFLP site. Weight of 20 days of HH genotype was significantly higher（P＜0.05）than that of hh genotype at the HinfⅠ-RFLP site, but groups divided by any other genotypes had no significant differences among other product performance.

Key words: H-FABP genotype, Hubei white pig, crossbred, Related trait