关键词: 禽流感, 血凝素, 转化, 百脉根

Abstract: 【Objiective】Lotus corniculatus is a kind of legume forage with good property. Avian influenza virus (AIV) hemagglutinin (H5N1) gene is transformed into the Lotus corniculatus in order to get the H5N1 antigen protein in it taken as the plant bioreactor. It will be helpful to manufacture the edible transgenic plant vaccine of AIV. It also has an important significance on spreading of AIV in wild migratory birds. 【Method】Taken its cotyledon petiole explant as the transformation receptors, we transform hemagglutinin gene of Avian Influenza to Lotus corniculatus by Agrobacterium-mediated transformation. After co-culture, selective differentiation and regeneration, we can get the resistant plant. Resistant plants were detected by PCR, RT-PCR and western blotting.【Result】The results prove that hemagglutinin gene has been transformed to the genome of Lotus corniculatus. Western blot analysis confirmed that hemagglutinin gene protein has been expressed on protein level in the transgenic plants and the result is better than the control.【Conclusion】It is feasible to use plant bioreactor to produce AIV H5N1 antigen protein in the legume forage.

Key words: Avian Influenza virus, hemagglutinin, Transformation, Lotus corniculatus L.