中国农业科学

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五指山小型猪实验用近交系培育研究成果

冯书堂   

  1. 中国农业科学院北京畜牧兽医研究所,北京 100094
  • 出版日期:2007-12-31 发布日期:2007-12-31
  • 作者简介:冯书堂(1945-),河北大名人,研究员,研究方向为动物干细胞及转基因技术。Tel:010-62815893 / 62899982;E-mail:fst508@sina.com
  • 基金资助:
    科技部“十五”攻关项目(2004BA717B);科技部社会公益性重点专项(2001DIA40036);科技基础性工作和社会公益研究专项(2003DEB6J078);“863”(No.2003AA205100);国家自然科学基金委重大、重点项目(339993420)、(39830190);“九五”农业部畜牧重点(95牧-02-08-03)、国家资源保种项目

The Inbreeds Culture of Chinese Wuzhishan Mini-pig Used for Experiments

FENG Shu-tang   

  1. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094
  • Online:2007-12-31 Published:2007-12-31

摘要: 历经近20年的努力,将我国体型最小、濒临灭绝的珍稀品种五指山小型猪(WZSP)异地保种获得成功,并培育出世界上首批近交系猪,已获近交F19,成年体重(24月龄)35~40 kg,其近交系数高达0.983。通过近交繁育与验证、生长发育规律、血液生理指标测定、分子遗传基础等研究发现:群体、家系微卫星座等位基因纯合度较高,SLA分型与人有较大的相似性,含有特异等位基因,不含MHSn 基因,GH和GH受体基因序列特异。初步揭示其矮小性、遗传稳定性、与人类相似性分子遗传基础;在人类比较医学,如烧伤植皮、心血管模型、口腔牙齿疾病、异种器官移植等深层次、多方位研究和开发利用结果表明:它不仅是肉猪育种、高档肉食加工的优良素材,更是实验动物模型研究的最佳选择。WZSP近交系在人类比较医学研究、异种器官移植研究中将发挥特殊的作用和不可估量的贡献。

关键词: 五指山小型猪 , 近交系 , 分子遗传

Abstract: Wuzhishan mini-pig (WZSP) is one of the scarce breeds, which is becoming extinct in China, the weight of sow is about 35-40 kg at 24-month-old of age. Since ex-situ breeding successful in WZSP and inbred line of WZSP in the world has made a progress by scientists of CAAS for nearly 20 years. Now, F19 has been obtained already, the inbreeding coefficient has reached 0.983. A series of studies have been carried out on germplam evaluation, laboratory rearing and inbreeding including inbreeding evaluation, performances of growth-development, evaluation of physiological and biochemical values, characteristics of molecular genetics,exploitation and application. The results shown that WZSP with small size, light body weight, early sexual maturity and high quality of meat production and also shown that WZSPs Microsatellite with Specific Allele, without MHSn gene, GH′s and GHR′s sequences is quite different from normal pigs, SLA types are similar to human and so on. It is not only the ideal material for pig’s meat breeding, but also the best choice for laboratory animals. It will make a special contribution to human model and xenotransplantation in the future.

Key words: Wuzhishan mini-pig , Inbred line , Molecular genetics