中国农业科学 ›› 2007, Vol. 40 ›› Issue (11): 2488-2494 .

• 植物保护 • 上一篇    下一篇

转基因抗虫棉叶围卡那霉素抗性细菌种群动态及nptⅡ基因漂移研究

邓 欣,赵廷昌,高必达   

  1. 中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室
  • 收稿日期:2006-10-30 修回日期:1900-01-01 出版日期:2007-11-10 发布日期:2007-11-10
  • 通讯作者: 高必达

Dynamics in kanamycin-resistant bacterial population and shift of nptII gene in the phyllosphere of insect-resistant transgenic cotton

  1. 中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室
  • Received:2006-10-30 Revised:1900-01-01 Online:2007-11-10 Published:2007-11-10

摘要: 【目的】研究转基因抗虫棉叶围卡那霉素抗性细菌的种群动态变化,并监测nptⅡ基因向叶围细菌漂移的情况。【方法】采用常规培养的方法分析转基因抗虫棉33B、SGK321和GK12、受体棉33、石远321和泗棉3号叶围具有卡那霉素抗性的细菌在整个生育期的种群动态变化,并以质粒pBI121为对照,根据nptⅡ基因设计特异性引物,采用菌落PCR方法对分离的叶围卡那霉素抗性细菌进行nptⅡ基因漂移监测。【结果】6个棉花品种的叶围卡那霉素抗性细菌总数量和群落多样性指数随着时间的变化,在同一棉花品种不同采样时间之间差异显著,而同一采样时间抗虫棉和对应的受体棉间多数差异不显著。在转基因抗虫棉品种SKG321,GK12及受体棉泗棉3号的叶围细菌中发现阳性片段。【结论】叶围卡那霉素抗性细菌的总数量及群落多样性指数在抗虫棉与对应的受体棉之间多数差异不显著。转基因抗虫棉中的nptⅡ基因可能向叶围细菌漂移。

关键词: 转基因抗虫棉, 叶围细菌, 卡那霉素抗性, 种群, nptⅡ

Abstract: 【Objective】To study the Dynamics in kanamycin-resistant bacterial population and monitor shift of nptII gene in the phyllosphere of insect-resistant transgenic cotton.【Method】Insect-resistant transgenic cotton 33B, SGK321 and GK12,and corresponding receptor cotton 33, Shiyuan 321 and No.3 Simian cotton were sampled at different growing stages. Dynamics and diversity among kanamycin-resistant bacterial populations in the phyllosphere were analyzed with the conventional culture methods. In addition, the shift of nptII gene was monitored by germ PCR, and the unique primer was designed from the sequence of nptII gene, the CK is pBI121 plasmids.【Result】at different sample time,significant differences were found among the total number and diversity index of phyllospere kanamycin-resistant bacterial population each cotton variety, but little differences between Insect-resistant transgenic cotton and corresponding receptor cotton at the same time. we discovered the positive segment from kanamycin-resistant bacteria in the phyllosphere of insect-resistant transgenic cotton SKG321, GK12 and receptor cotton No.3 Simian cotton.【Conclusion】We suggested that little differences were found among the total number and diversity index of phyllospere kanamycin-resistant bacterial population between Insect-resistant transgenic cotton and corresponding receptor cotton,and nptII gene in insect-resistant transgenic cottons could drift into the phyllosphere bacteria.

Key words: insect-resistant transgenic cotton, phyllosphere bacteria, kanamycin-resistant, population, biosafety, nptII