关键词: 小尾寒羊, 痒病, 单抗, 免疫组化

Abstract: 【Objective】The method of immunohistochemistry assay for the detection of Scrapie by monoclonal antibody was established.【Method】Genomic DNA was isolated from the ovine whole blood. Using the polymerase chain reaction to amplify the core part of PrP from the ovine Genomic DNA. By using the recombinant DNA technology, the recombinant protein of core part of PrP was obtained. Then using standard methodology of myeloma cell fusion, monoclonal antibodies were obtained to detect Scrapie by immunohistochemistry.【Results】The recombinant protein of core part of ovine PrP was obtained and a panel of six hybridoma cell lines secreting specific antibodies to core part of PrP related to Scrapie were established from one fusion between myeloma Sp2/0 and spleen cells from mice immunized with the purified recombinant protein. Four hybridoma cell lines can be used in immunohistochemistry assay for the detection of Scrapie.【Conclusion】The recombinant core part of PrP and special Mabs was obtained by using recombinant DNA and myeloma cell fusion technology. So the special monoclonal antibody developed in authors university can be used to detect PrPsc of Scrapie by immunohistochemistry.

Key words: Ovine, Scrapie, Monoclonal antibody, Immunohistochemistry