关键词: 小麦, SacB基因, 转化, 耐旱

Abstract: 【Objective】SacB gene from Bacillus subtilis will be transferred into wheat cultivars or breeding lines 02-371, 02-207, Zhengmai 9023 and Dongnong 7742 for improving their tolerance to drought stress. 【Method】Using wheat immature embryo calli as explants, SacB gene was transferred into four wheat cultivars by Agrobacterium-mediated transformation. Transformed plants were detected by PCR and Southern blotting. 【Result】 PCR and Southern blotting analysis showed that the SacB gene has been integrated into wheat genome. One or two copies of integrated transgene were in transgenic plants. The transformation frequencies of Line 02-371, Line 02-207, Zhengmai 9023 and Dongnong 7742 were 0.88%, 1.48%, 1.04%, and 1.23%, respectively. RT-PCR analysis showed that the SacB gene was expressed in some transgenic plants, of which the tolerance to drought stress was significantly improved. 【Conclusion】 SacB gene from Bacillus subtilis can improve the tolerance of transgenic wheat plants to drought stress.

Key words: Wheat (Triticum aestivum L.), SacB gene, Transformation, Drought tolerance