关键词: 口蹄疫病毒, 非结构蛋白, VPg2-ELISA方法评价

Abstract: VPg2 gene fragment was successfully subcloned from 3ABC gene of the foot-and-mouth disease virus（FMDV）TaiBao strain and constructed VPg2 expressing plasmid by inserting the target gene fragment into pGEX-4T-1 vector. The expressed protein was analysed by Western blotting. The result showed strong reaction with FMDV positive serum. VPg2 protein ELISA was evaluated through testing sera of 4 cattle groups i.e. control group, vaccinated group, infected group and infected after vaccination group. Negative results were obtained for the control group and the vaccinated group. On the contrary, positive results were obtained for the infected group and part of the infected after vaccination group. In the infected group and infected after vaccination group, the duration of the antibody against VPg2 lasted for at least 90 days. The earliest detecting time of VPg2 antibody was 10th day after infection. The duration of the antibody against VPg2 was the same with the antibody against 3ABC tested previously. 2170 negative sera were tested using indirect VPg2-ELISA and 3ABC-ELISA. The results showed that the false positive rates were 0.55% in VPg2 ELISA assay and were 2.21% in 3ABC-ELISA assay.

Key words: Foot-and-mouth disease virus, Non-structural protein, Evalulation of VPg2-ELISA