关键词: 草鱼, MyoD基因, 克隆

Abstract: Total RNA was isolated from grass carp embryos at 22 h post-fertilization to clone the MyoD cDNA by using rapid cDNA end amplification, and the sequence was analyzed with other animals. The results showed that: the full-length of grass carp MyoD is 1 597 bp, with 825 bp open read frame enconding 275 amino acids, including a bHLH domain which composes of 1th to 84th amino acids and 98th to 141th amino acids, without signal peptide; Compared grass carp MyoD with other animals, the results showed that the length of MyoD peptide is related to the evolution of animal, and the homology of nucleotide and amino acid accords with genetic relationship among the animals; Cloning of grass carp MyoD is meaningful for researching the mechanism of fish's muscle development and dominate, it is also helpful for improving the muscle quality of fish artificially.

Key words: grass carp, MyoD gene, Cloning