中国农业科学 ›› 2005, Vol. 38 ›› Issue (04): 719-724 .

• 植物保护 • 上一篇    下一篇

朱砂叶螨抗甲氰菊酯品系选育及遗传分析

何林,赵志模   

  1. 西南农业大学植物保护学院
  • 收稿日期:2004-05-10 修回日期:1900-01-01 出版日期:2005-04-10 发布日期:2005-04-10
  • 通讯作者: 赵志模

Resistance Selection of Tetranychus cinnabarinus to Fenpropathrin and Genetic Analysis

,,   

  1. 西南农业大学植物保护学院
  • Received:2004-05-10 Revised:1900-01-01 Online:2005-04-10 Published:2005-04-10

摘要: 从保留的室内相对敏感品系分出一个亚品系开始培育朱砂叶螨的甲氰菊酯抗药性品系。连续用药40代,抗性增长68.5倍。以此抗性品系(R)和相对敏感品系(S)杂交、回交,研究朱砂叶螨对甲氰菊酯的抗性遗传形式。正交(SR)和反交(RS)F1代的LC-P线介于S与R之间且偏向S方,显性度DSR和DRS分别为-0.83和-0.29,表明抗性由不完全隐性基因控制;两D值(DRS和DSR)95%置信限没有重叠,表明两个 D值(DSR和DRS)存在显著差异,也表明朱砂叶螨对甲氰菊酯的抗性遗传可能存在母体影响或核外效应;对F1杂合子与亲本的回交(SR♀×S♂和RS♀×R♂)F2代测定表明抗性由单基因控制。

关键词: 朱砂叶螨, 抗药性, 甲氰菊酯, 遗传形式

Abstract: The resistant inheritance mode of Tetranychus cinnabarinus to fenpropathrin were studied based on the resistance selection in laboratory. After 40 generations selection, T. cinnabarinus developed 68.5-fold resistance to fenpropathrin. The methods of cross and back-cross between resistant (R) and sensitive (S) strains were used for exploring the inheritance mode of the resistance of this mite to fenpropathrin. The log (LC)-P equation of F1 got from obverse (SR) and reverse (RS) crosses was intermediate between S and R and inclined to S, the dominant indices DSR and DRS were - 0.83 and - 0.29, respectively, which indicated that the resistance is controlled by the incompletely recessive gene; the 95% confidence limit of this two dominant indices do not superpose showing DSR and DRS have significant difference and the maternal or external karyon effect may be existed in resistance inheritance; the back-cross results of heterozygote F1 with its parents suggested that the separation of progenies F2 (SR♀× S♂ and RS♀× R♂) was consistent with Mendel's single gene inheritance model.

Key words: Tetranychus cinnabarinus, Resistance, Fenpropathrin, Inheritance mode