中国农业科学 ›› 2004, Vol. 37 ›› Issue (10): 1464-1467 .

• 耕作栽培.生理生态 • 上一篇    下一篇

不同大麦淀粉胚乳细胞中淀粉和贮藏蛋白的积累

  

  • 收稿日期:2003-07-21 修回日期:2003-11-19 出版日期:2004-10-20 发布日期:2004-10-20
  • 通讯作者: 兰盛银

  1. 华中农业大学生命科学技术学院
  • Received:2003-07-21 Revised:2003-11-19 Online:2004-10-20 Published:2004-10-20

关键词: 大麦, 胚乳, 淀粉体, 蛋白质体, 小淀粉粒, 细胞结构

Abstract: Three barley subvarieties Hua7618 (non-waxy), 20A2 (waxy) and Hua2012 (osculant subvariety), which were different in endosperm types, were used in this report. During endosperm development, the initiation and accumulation of starch and storage protein as well as their morphological changes in endosperm cells of those subvarieties were observed sequentially by optical microscope and transmission electron microscope. The results showed that in Hua7618 endosperm cells, the size increase of large starch granules was the quickest and the average volume of them till grain maturation was the largest compared with the other subvarieties, while the 1 000-grain weight and the number of small starch granules were the greatest and the content of protein was the least. The situation in 20A2 endosperm cells was just opposite to Hua7618, and the situation in Hua2012 was intervenient. One amyloplast contained one large starch granule in endosperm cells, its number hardly increased after 13 days after flowering. Protein bodies were dispersed spherical particles at the prophase of caryopsis development. They fused with each other at the anaphase of caryopsis development and deposited among starch granules. Nuclei in endosperm cells deformed gradually at the metaphase of caryopsis development, and the initiation of small starch granules occurred during or after nuclei disruption.