球孢白僵菌对烟粉虱后代生命表参数的亚致死影响

doi:10.3864/j.issn.0578-1752.2014.18.007

中国农业科学 ›› 2014, Vol. 47 ›› Issue (18): 3588-3595.doi: 10.3864/j.issn.0578-1752.2014.18.007

球孢白僵菌对烟粉虱后代生命表参数的亚致死影响

王登杰¹，臧连生²，张烨³，王海鸿¹，雷仲仁¹

¹中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室，北京 100193
²吉林农业大学农学院，长春 130118；³山西省农业科学院植物保护研究所，太原030031

收稿日期:2014-02-19 修回日期:2014-03-24 出版日期:2014-09-16 发布日期:2014-09-16
通讯作者: 王海鸿，Tel：010-62815930；E-mail：wanghaihong2020@sina.com 雷仲仁，Tel：010-62815930；E-mail：leizhr@sina.com
作者简介:王登杰，576023312@qq.com
基金资助:
现代农业产业技术体系专项资金（CARS-25-B-07）
北京市科技计划（Z121100001212006）
公益性行业（农业）科研专项（201303019-02）

Sublethal Effects of Beauveria bassiana Balsamo on Life Table Parameters of Subsequent Generations of Bemisia tabaci Gennadius

WANG Deng-jie¹, ZANG Lian-sheng², ZHANG Ye³, WANG Hai-hong¹, LEI Zhong-ren¹

¹Institute of Plant Protection, Chinese Academy of Agricultural Sciences/State Key Laboratory for Biology of Plant Diseases and Insect Pests, Beijing 100193 ²Faculty of Agronomy, Jilin Agricultural University, Changchun 130118; ³Institute of Plant Protection, Shanxi Academy of Agricultural Sciences, Taiyuan 030031

Received:2014-02-19 Revised:2014-03-24 Online:2014-09-16 Published:2014-09-16

摘要/Abstract

摘要： 【目的】关于昆虫病原真菌对昆虫生命表亚致死影响的研究较少，而由真菌侵染所产生的亚致死影响对于寄主昆虫的种群动力学至关重要，并最终影响着目标昆虫的害虫地位。论文旨在比较烟粉虱（Bemisia tabaci）亲代3龄若虫经球孢白僵菌（Beauveria bassiana）处理及对照子代种群的生命表参数，探讨球孢白僵菌对烟粉虱生命表参数的亚致死影响。【方法】应用年龄-阶段两性生命表分析经球孢白僵菌处理的烟粉虱后代的发育历期、存活率和繁殖率数据，以蒸馏水处理的个体作为对照。应用bootstrap方法计算种群生长参数的平均数和标准误；采用t-test比较分析球孢白僵菌处理组和对照组烟粉虱的种群参数、发育历期和繁殖力间的差异。【结果】球孢白僵菌处理的烟粉虱与对照之间在成虫前期、总产卵前期、1—4龄若虫存活率和生命表参数上存在着显著差异。与对照处理的烟粉虱成虫前期（20.59 d）相比，亲代接种球孢白僵菌使后代个体的成虫前期显著延长（26.58 d）。经球孢白僵菌处理的烟粉虱后代存活率（0.195）要比对照个体（0.76）低。球孢白僵菌处理烟粉虱后代种群的内禀增长率（r）、周限增长率（λ）、净增殖率（R₀）、平均世代时间（T）和总繁殖率（GRR）分别为0.063 d^-1、1.065 d^-1、6.85，30.613 d和41.883，而对照烟粉虱后代种群的上述参数分别为0.137 d^-1、1.147 d^-1、33.443、25.575 d和51.44。【结论】亲代经球孢白僵菌处理的烟粉虱其子代种群增长要比对照组慢，死亡率高，但生殖能力并无显著性影响。该研究可为了解昆虫病原真菌对烟粉虱的长期影响提供理论依据。

关键词: 球孢白僵菌, 烟粉虱, 生命表, 亚致死影响

Abstract: 【Objective】 Beauveria bassiana (Bals.) Vuill., an important entomopathogenic fungus, has been widely used to control Bemisia tabaci Gennadius. No much research has been conducted to study the sublethal effects of fungus on life table parameters generations of insects, while sublethal effects of fungal infection can have important implications for the population dynamics of the host offsprings, which ultimately contributes to the status of the target insect as a pest. The objective of this study is to compare life tables of subsequent generations of B. tabaci arising from parental generations exposed in the third nymphal stages to B. bassiana and controls, and to investigate the sublethal effects of fungus on life table parameters of B. tabaci offsprings. 【Method】 Developmental, survival, and fecundity rate data were analyzed by using the age-stage, two-sex life table in fungus-treated B. tabaci. Those exposed to distilled water were considered as control. Means and standard errors of population growth parameters were calculated by using the bootstrap method. The t-test was used to evaluate the differences in the population parameters, development times, and fecundities between treated B. tabaci and control. 【Result】 There were significant differences in pre-adult developmental time, total preoviposition period, 1st-4th instars survival and life table parameters between fungus-treated whiteflies and control. In comparison to controls (20.59 d), parental generations exposed to fungus caused longer pre-adult development times (26.58 d). Fungus exposure caused lower survival rate (0.195) for subsequent generations individuals than control (0.76). The intrinsic rate of increase (r), finite rate (λ), net reproductive rate (R₀), mean generation time (T) and gross reproductive rate (GRR) were 0.063 d^-1，1.065 d^-1, 6.85，30.613 d and 41.883, respectively, for treated whiteflies and 0.137 d^-1，1.147 d^-1，33.443，25.575 d and 51.44, respectively, for control. The population growth of fungus-treated B. tabaci was slower than control. 【Conclusion】 Offsprings of fungus-treated B. tabaci compared to control increased their population slower, had higher mortality. However, fungal treatment didn’t impose significant effect on fecundity of B. tabaci. This information can provide insights into the long-term effects of applied entomopathgenic fungi on B. tabaci populations.

Key words: Beauveria bassiana, Bemisia tabaci, life table, sublethal effects

王登杰，臧连生，张烨，王海鸿，雷仲仁. 球孢白僵菌对烟粉虱后代生命表参数的亚致死影响[J]. 中国农业科学, 2014, 47(18): 3588-3595.

WANG Deng-jie, ZANG Lian-sheng, ZHANG Ye, WANG Hai-hong, LEI Zhong-ren. Sublethal Effects of Beauveria bassiana Balsamo on Life Table Parameters of Subsequent Generations of Bemisia tabaci Gennadius[J]. Scientia Agricultura Sinica, 2014, 47(18): 3588-3595.

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参考文献

[1] Brown J K, Frohlich D R, Rosell R C. The sweetpotato or silverleaf whiteflies: biotypes of Bemisia tabaci or a species complex? Annual Review of Entomology, 1995, 40: 511-534.

[2] Osborne L S, Landa Z. Biological control of whiteflies with entomopathogenic fungi. Florida Entomologist, 1992, 75: 456-471.

[3] Faria M, Wraight S P. Biological control of Bemisia tabaci with fungi. Crop Protection, 2001, 20: 767-778.

[4] Varma A, Malathi V A. Emerging geminivirus problems: A serious threat to crop production. Annals of Applied Biology, 2003, 142: 145-164.

[5] Perring T M. The Bemisia tabaci species complex. Crop Protection, 2001, 20(9): 725-737.

[6] Boykin L M, Shatters R G, Rosell R C, McKenzie C L, Bagnall R A, De Barro P, Frohlich D R. Global relationship of Bemisia tabaci (Hemiptera: Aleyrodidae) revealed using Bayesian analysis of mitochondrial COI DNA sequences. Molecular Phylogenetics and Evolution, 2007, 44: 1306-1319.

[7] Guiral P, Beitia F, Cenis J L. Biotype determination of Spanish populations of Bemisia tabaci on cotton in Isreal. Environmental Entomology, 1992, 21: 556-559.

[8] Wang Z, Yan H, Yang Y, Wu Y. Biotype and insecticide resistance status of the whitefly Bemisia tabaci from China. Pest Management Science, 2010, 66: 1360-1366.

[9] 闫文茜, 王相晶, 张友军, 王少丽. 北京地区蔬菜烟粉虱种群动态及其对烟碱类杀虫剂的抗药性监测. 植物保护, 2012, 38(5): 154-157.

Yan W Q, Wang X J, Zhang Y J, Wang S L. Population dynamics of the vegetable whitefly, Bemisia tabaci, and its resistance to neonicotinoid insecticides in Beijing area. Plant Protection, 2012, 38(5): 154-157. (in Chinese)

[10] McCoy C W, Samson R A , Boucias D G. Entomogenous fungi// Ignoffo C M, Mandava N B. Handbook of Natural Pesticides, Vol. V: Microbial Insecticides, Part A: Entomogenous Protozoa andFungi. Boca Raton, FL: CRC Press, 1988: 151-236.

[11] 张烨, 雷仲仁, 王海鸿, 吉青战. 球孢白僵菌HsbA蛋白的原核表达及免疫定位. 中国农业科学, 2013, 46(21): 4534-4541.

Zhang Y, Lei Z R, Wang H H, Ji Q Z. Prokaryocyte expression and immune localization of HsbA in Beauveria bassiana. Scientia Agricultura Sinica, 2013, 46(21): 4534-4541. (in Chinese)

[12] Liu Z, Lei Z, Hua B, Wang H, Liu T. Germination Behavior of Beauveria bassiana (Deuteromycotina: Hyphomycetes) on Bemisia tabaci (Hemiptera: Aleyrodidae) nymphs. Journal of Entomology Science, 2010, 45(4): 322-334.

[13] Wraight S P, Carruthers R I, Bradley C A, Jaronski S T, Lacey L A, Wood P, Galaini-Wraight S. Pathogenitity of the entomopathogenic fungi Paecilomyces spp. and Beauveria bassiana against the silverleaf whitefly, Bemisia argentifolii. Journal of Invertebrate Pathology, 1988, 71: 217-226.

[14] Wraight S P, Carruthers R I, Jaronski S T, Bradley C A, Garza C J, Galaini-Wraight S. Evaluation of the entomopathogenic fungi Beauveria bassiana and Paecilomyces fumosoroseus for microbial control of the silverleaf whitefly, Bemisia argentifolii. Biological Control, 2000, 17: 203-217.

[15] Quesada-Moraga E, Maranhao E A A, Valverde-Garcîa, Santiago- Álvarez C. Selection of Beauveria bassiana isolate for control of the whiteflies Bemisia tabaci and Trialeurodes vaporariorum on the basis of the virulence, thermal requirements, and toxicogenic activity. Biological Control, 2006, 36: 274-287.

[16] Liu T X, Stansly P A. Effects of relative humidity on efficacy of BotaniGuard^TM (Beauveria bassiana) on nymphs of sweetpotato whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae) on hibiscus in greenhouses. Southwest Entomologist, 2009, 32: 189-191.

[17] 刘召. 白僵菌加1菌株生物学习性及其对烟粉虱致病机理的初步研究[D]. 杨凌: 西北农林科技大学, 2006.

Liu Z. Biological characteristics of Beauveria bassiana isolate Canada 1 and its pathogenesis to Bemisia tabaci[D]. Yangling: Northwest A&F University, 2006. (in Chinese)

[18] Quesada-Moraga E, Santos-Quirós R, Valverde-Garcîa, Santiago- Álvarez C. Virulence, horizontal transmission, and sublethal reproductive effects of Metarhizium anisopliae (Anamorphic fungi) on the German cockroach (Blattodea: Blattellidae). Journal of Invertebrate Pathology, 2004, 87: 51-58.

[19] Latifian M, Soleimannejadian E, Ghazavi M, Mosadegh M S, Hayati J. Effects of sublethal concentrations of fungus Beauveria bassiana on the reproductive potentials of sawtoothed beetle Oryzaephilus surinamensis on commercial date cultivars. Plant Protection Journal, 2010, 2(4): 279-292.

[20] Seyed-Talebi F S, Kheradmand K, Talaei-Hassanloui R, Talebi- Jahromi K. Sublethal effects of Beauveria bassiana on life table parameters of two-spotted spider mite, Tetranychus urticae (Acari: Tetranychidae). Biocontrol Science and Technology, 2012, 22(3): 293-303.

[21] Torrado-León E, Montoya-Lerma J, Valencia-Pizo E. Sublethal effects of Beauveria bassiana (Balsamo) Vuillemin (Deuteromycotina: Hyphomycetes) on the white?y Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) under laboratory conditions. Mycopathologia, 2006, 162: 411-419.

[22] Chi H, Su H Y. Age-stage, two-sex life tables of Aphidius gifuensis (Ashmead) (Hymenoptera: Braconidae) and its host Myzus persicae (Sulzer) (Homoptera: Aphididae) with mathematical proof of the relationship between female fecundity and the net reproductive rate. Environmental Entomology, 2006, 35(1): 10-21.

[23] 罗晨, 姚远, 王戎疆, 阎凤鸣, 胡敦孝, 张芝利. 利用mtDNA Co I基因序列鉴定我国烟粉虱的生物型. 昆虫学报, 2002, 45(6): 759-763.

Luo C, Yao Y, Wang R J, Yan F M, Hu D X, Zhang Z L. The use of mitochondrial cytochrome oxidase I (mt Co I) gene sequences for the identification of biotypes of Bemisia tabaci (Gennadius) in China. Acta Entomologica Sinica, 2002, 45(6): 759-763. (in Chinese)

[24] 臧连生, 刘银泉, 刘树生. 一种适合粉虱实验观察的新型微虫笼. 昆虫知识, 2005, 42(3): 329-331.

Zang L S, Liu Y Q, Liu S S. A new clip-cage for whitefly experimental studies. Chinese Bulletin of Entomology, 2005, 42(3): 329-331. (in Chinese)

[25] Chi H, Liu H. Two new methods for the study of insect population ecology. Bulletin of the Institute of Zoology Academia Sinica, 1985, 24(2): 225-240.

[26] Chi H. Life-table analysis incorporating both sexes and variable development rates among individuals. Environmental Entomology, 1988, 17(1): 26-34.

[27] Chi H. TWOSEX-MSChart: a computer program for the age-stage, two-sex life table analysis. http://140.120.197.173/Ecology/Download/ Twosex-MSChart.zip.

[28] Efron B, Tibshirani R J. An Introduction to the Bootstrap. New York: Chapman and Hall, 1993.

[29] Lewontin R C. Selection for colonizing ability//Baker H G, Stebbins G L. The Genetics of Colonizing Species. Academic, San Diego, California, 1965: 77-91.

[30] 任洁, 韩雪梅, 刘昭, 雷仲仁. 烟粉虱血细胞对白僵菌的防御反应. 中国蔬菜, 2013(12): 61-65.

Ren J, Han X M, Liu Z, Lei Z R. Defense response of hemolymph in Bemisia tabaci to Beauveria bassiana. China Vegetables, 2013(12): 61-65. (in Chinese)

[31] Sharma S, Agarwal G P, Rajak R C. Pathophysiological alterations caused in Heliothis armigera by toxic metabolites of Beauveria bassiana (Bals) Vuill. Indian Journal of Experimental Biology, 1994, 32: 168-171.

[32] Davidson E W, Patron R B R, Lacey L, Frutos R, Vey A, Hendrix D L. Activity of natural toxins against the silverleaf whitefly, Bemisia argentifolii, using a novel feeding bioassay system. Entomologia Experimentalis et Applicata, 1996, 79(1): 25-32.

[33] Moore D, Reed M, Le Patourel G, Abraham Y J, Prior C. Reduction of feeding by the desert locust, Schistocerca gregaria, after infection with Metarhizium flavoviridae. Journal of Invertebrate Pathology, 1992, 60(3): 304-307.

[34] Villani M, Krueger G, Schroeder S R, Consolie P C, Consolie N H, Preston-Wilsey L M, Roberts D W. Soil application effects of Metarhizium anisopliae on Japanese beetle (Coleoptera: Scarabeidae) behavior and survival in turfgrass microcosms. Environmental Entomology, 1994, 23: 502-503.

[35] Lacey L A, Mesquita A L, Mercadier G, Debire R, Kazmer D J, Leclant F. Acute and sublethal activity of the entomopathogenic fungus Paecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes) on adult Aphelinus asychis (Hymenoptera: Aphelinidae). Environmental Entomology, 1997, 26: 1452-1460.

[36] Poprawski T J, Legaspi J C, Parker P E. Influence of entomopathogenic fungi on Serangium parcesetosum (Coleoptera: Coccienlidae), an important predator of whiteflies (Homoptera: Aleyrodidade). Environmental Entomology, 1982, 27(3): 785-795.

球孢白僵菌对烟粉虱后代生命表参数的亚致死影响

Sublethal Effects of Beauveria bassiana Balsamo on Life Table Parameters of Subsequent Generations of Bemisia tabaci Gennadius

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