中国农业科学 ›› 2014, Vol. 47 ›› Issue (14): 2762-2773.doi: 10.3864/j.issn.0578-1752.2014.14.007

• 植物保护 • 上一篇    下一篇

中国主要稻区稻曲病菌的生物学特性及群体遗传多样性

 王文斌1, 2, 张荣胜2, 罗楚平2, 尹小乐2, 刘永锋2, 陆凡1, 2, 陈志谊1, 2   

  1. 1、南京农业大学植物保护学院,南京210095;
    2、江苏省农业科学院植物保护研究所,南京210014
  • 收稿日期:2014-01-24 出版日期:2014-07-15 发布日期:2014-04-02
  • 通讯作者: 陈志谊,Tel:025-84390393;E-mail:chzy@jaas.ac.cn;陆凡,Tel:025-84390388;E-mail:lufan@jaas.ac.cn
  • 作者简介:王文斌,Tel:025-84390230;E-mail:wwb1212cs001@163.com
  • 基金资助:

    公益性行业(农业)科研专项(200903039-2)、国家自然科学基金(31171802)

Biological Characteristics and Genetic Diversity of Ustilaginoidea virens from Rice Regions in China

 WANG  Wen-Bin-1, 2 , ZHANG  Rong-Sheng-2, LUO  Chu-Ping-2, YIN  Xiao-Le-2, LIU  Yong-Feng-2, LU  Fan-1, 2 , CHEN  Zhi-Yi-1, 2   

  1. 1、College of Plant Protection, Nanjing Agricultural University, Nanjing 210095;
    2、Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014
  • Received:2014-01-24 Online:2014-07-15 Published:2014-04-02

摘要: 【目的】明确采自中国10个水稻主产省份111个稻曲病菌(Ustilaginoidea virens)菌株的生物学特性、群体遗传多样性与地理区域的关系。【方法】采用生物学方法测定111个稻曲病菌菌株的产孢能力、生长速率和致病力,并采用SPSS 20.0软件对稻曲病菌菌株的产孢能力、菌丝生长速率及致病力进行相关性分析。提取111个稻曲病菌基因组DNA,采用3对特异性引物BOX、REP、ERIC对稻曲病菌菌株的全基因组DNA进行PCR扩增,通过聚类分析对其群体遗传多样性进行研究。【结果】111个稻曲病菌菌株的致病力与产孢量的Pearson相关系数为0.765,显著性概率为0.001;致病力与生长速率的Pearson相关系数为0.035,显著性概率为0.685。采用3对特异性引物BOX、ERIC、REP对稻曲病菌菌株的全基因组DNA进行了PCR扩增,群体遗传多样性值分别为0.73、0.62和0.60。111个稻曲病菌菌株的DNA用BOX引物分别扩增出7—13条不等的带谱,DNA指纹相似性在0.70相似水平上,供试菌株被划分为6种基因类群,其中第1类群为优势类型,有57个菌株,占总数的51.4%;来自安徽、四川、广西、湖北和云南的菌株主要划分在类群1和4,第2类群主要是来自江苏的菌株,有18个菌株,占总数的16.2%;第3类群是来自浙江的菌株,有8个菌株,占总数的7.2%;第5类群是来自黑龙江的菌株,有9个菌株,占总数的8.1%,第6类群是来自辽宁的菌株,有12个菌株,占总数的10.8%。稻曲病菌基因类群与地理区域之间有一定的相关性,与致病力、生长速率和产孢量之间没有相关性。【结论】来自中国10个省份的111个稻曲病菌菌株的致病力与产孢量之间具有显著相关性,相关程度为中等;致病力与菌丝的生长速率没有相关性。根据BOX-PCR扩增的稻曲病菌菌株基因组DNA指纹图谱的多态性进行划分的基因类群与地理区域之间显著相关,推测稻曲病菌属于局域性传播;基因类群与生物学特性之间没有相关性。

关键词: 稻曲病菌 , 生物学特性 , 地理区域 , BOX-PCR , 群体遗传多样性

Abstract: 【Objective】The objective of this study is to reveal the relationship among geographical regions, biological characteristics and genetic diversity of 111 strains of Ustilaginoidea virens from 10 provinces in China.【Method】Sporulation, growth rate and pathogenicity of these strains were evaluated by biological methods, and the relativity was analyzed by using the software SPSS 20.0. DNA was extracted from the strains, the genomic fingerprint profiles of 111 strains were generated by BOX-PCR, REP-PCR and ERIC-PCR, respectively. Their genetic diversities were further investigated by using clustering analysis.【Result】The correlation coefficient of sporulation and pathogenicity was 0.765 and the significance probability was 0.001, the correlation coefficient of growth rate and pathogenicity was 0.035 and the significance probability was 0.685. The genomic fingerprint profiles of 111 strains were generated by BOX-PCR, REP-PCR and ERIC-PCR, respectively. The genetic diversities of the U. virens population were 0.73 (in BOX), 0.62 (in ERIC) and 0.60 (in REP), respectively. The number of polymorphic bands was between 7 and 13 of each examined isolate detected through BOX-PCR analysis. According to cluster analysis of UPGMA, 6 clusters were grouped based on the boundary level of 0.70 average distances. The predominant group was group one which included 57 strains (51.4%). The strains of U. virens from Anhui, Sichuan, Guangxi, Hubei and Yunnan were grouped into group 1 and group 4. The strains of group 2 were mainly from Jiangsu, including 18 strains (16.2%), the strains of group 3 were from Zhejiang, including 8 strains (7.2%), the strains of group 5 were from Heilongjiang, including 9 strains (8.1%), and the strains of group 6 were from Liaoning, including 12 strains (10.8%). There was some correlation between BOX groups and geographic regions, no relationships were observed between BOX groups and their biological characteristics.【Conclusion】The relativity between the sporulation and pathogenicity was assumed to be medium. There was no relativity between the growth rate and pathogenicity. There was a significant relationship between BOX groups and the geographical regions, these results suggested that, naturally, U. virens in China might not be spread over long distances. No significant relationships were observed between BOX groups and their biological characteristics.

Key words: Ustilaginoidea virens , biological characteristics , geographical regions , BOX-PCR , genetic diversity