碳氮比对鸡粪堆肥中土霉素降解和堆肥参数的影响

doi:10.3864/j.issn.0578-1752.2013.07.010

摘要/Abstract

摘要： 【目的】分析不同C/N处理下土霉素在鸡粪堆肥中的降解及其对堆肥理化性质的影响，以期阐明不同C/N处理对土霉素降解及堆肥腐熟度的影响。【方法】以鸡粪和小麦秸秆为原料，采用室内好氧堆肥法，研究鸡粪好氧堆肥过程中，不同C/N处理（T1：C/N=21.6、T2：C/N=25.5和T3：C/N=32.8）对鸡粪堆肥中土霉素的降解及堆体温度、pH、发芽指数等指标的影响。【结果】（1）C/N对鸡粪堆肥过程中土霉素的降解影响显著。整个堆肥期内，不同处理土霉素的降解速率大小顺序为T2＞T3＞T1。土霉素在各处理堆体中的降解均符合一级动力学方程，其相关系数介于0.9431—0.9967。（2）土霉素存在条件下，堆肥初始C/N对堆体温度是有影响的。C/N高的处理较C/N低的处理堆体温度上升速率快, 达到最高温所需的时间也较短，最高温也较高。（3）C/N对土霉素存在下鸡粪堆肥堆体可溶性氮含量影响显著。如堆肥结束时T1、T2和T3处理堆体NO3--N与堆肥初始相比分别升高78.50%、62.37%和59.34%。（4）高C/N处理在一定程度上可以显著降低堆肥浸提液的生物毒性。如堆肥结束时C/N为25.5和32.8的处理GI＞80%。【结论】对含有土霉素的鸡粪进行堆肥，较高的初始C/N（25.5-32.8）能够有效地促进鸡粪中土霉素的降解和鸡粪的腐熟。

关键词: 碳氮比 , 鸡粪 , 土霉素 , 好氧堆肥 , 理化参数

Abstract: 【Objective】 The effects of C/N on the OTC degradation and the physicochemical characters and maturity of chicken composts during chicken feces composting were studied.【Method】 The indoor aerobic composting method was used in the present paper. Three groups of composting materials with C/N ratios of 21.6, 25.5 and 32.8 were prepared through combinations of chicken manure and wheat straw. To illustrate the effects of C/N on OTC degradation and composting process, OTC contents of chicken feces and the selected parameters such as pile temperature, pH, EC, contents of NO3--N, and NH4+-N, and germination index(GI) in composting process were investigated. 【Result】 It was found that C/N has significant effects on the degradation of OTC, and the order of OTC degradation was T2＞T3＞T1. The degradation of OTC in chicken feces during composting could be well described by the first-order kinetic model, and the correlation coefficients ranged from 0.9431 to 0.9967. The effects were found of C/N on pile temperature during composting. The high C/N treatments had higher speed of temperature rising and the shorter time for temperature rising than the low C/N treatment. The highest pile temperatures were significantly higher than that of the low C/N treatment. Effects were also found of C/N on the soluble nitrogen contents of piles during composting. For example, the content of NO3--N of three treatments were increased by 78.50%, 62.37%, and 59.34% at the end of composting, compared with their values at the beginning of composting. High C/N treatment could significantly reduce the biotoxicity of compost. For example, the treatments with C/N ratios of 25.5 and 32.8 had high GI values (The GI values are much higher than 80%).【Conclusion】The highly initial C/N ranging from 25.5-32.8 could significantly decrease the OTC in chicken feces and be benefit for the chicken feces maturity.

Key words: C/N , chicken feces , oxytetracycline , aerobic composting , physicochemical parameters

王桂珍, 李兆君, 张树清, 马晓彤, 梁永超. 碳氮比对鸡粪堆肥中土霉素降解和堆肥参数的影响[J]. 中国农业科学, 2013, 46(7): 1399-1407.

WANG Gui-Zhen, LI Zhao-Jun, ZHANG Shu-Qing, MA Xiao-Tong, LIANG Yong-Chao. Influence of C/N Ratio on Degradation of Oxytetracycline and Composting Parameters During Chicken Manure Composting[J]. Scientia Agricultura Sinica, 2013, 46(7): 1399-1407.

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https://www.chinaagrisci.com/CN/Y2013/V46/I7/1399

参考文献

[1]Kumar K, Gupta S C, Baidoo S K, Chander Y, Rosen C J. Antibiotic uptake by plants from soil fertilized with animal manure. Journal of Environmental Quality, 2005, 34(6): 2082-2085.

[2]张树清. 规模化养殖畜禽粪有害成分测定及其无害化处理效果[D]. 北京: 中国农业科学院, 2004.

Zhang S Q. Analysis of main harmful components in manures of livestock and poultry from intensive feedlots[D]. Beijing: Chinese Academy of Agricultural Sciences, 2004. (in Chinese）

[3]Kumar K, Gupta S C, Chander Y, Singh A K. Antibiotic use in agriculture and its impact on the terrestrial environment. Advances in Agronomy, 2005, 87: 1-54.

[4]姚建华, 牛德奎, 李兆君, 梁永超, 张树清. 抗生素土霉素对小麦根际土壤酶活性和微生物生物量的影响. 中国农业科学, 2010, 43(4): 721-728.

Yao J H, Niu D K, Li Z J, Liang Y C, Zhang S Q. Effects of the antibiotic oxytetracycline on soil enzyme activities and microbial biomass in wheat rhizosphere. Scientia Agricultura Sinica, 2010, 43(4): 721-728. (in Chinese)

[5]Heuer H, Schmitt H, Smalla K. Antibiotic resistance gene spread due to manure application on agricultural fields. Current Opinion in Microbiology, 2011, 14: 236-243.

[6]解晓瑜, 张永清, 李兆君, 梁永超, 姚建华, 张树清. 兽用土霉素对小麦毒理效应的基因型差异研究. 生态毒理学报, 2009, 4(4): 577-583.

Xie X Y, Zhang Y Q, Li Z J, Liang Y C,Yao J H, Zhang S Q. Toxicological of veterinary oxytetracycline and the genotypic differences on wheat. Journal of Ecotoxicology, 2009, 4(4): 577-583. (in Chinese)

[7]Li Z J, Xie X Y, Zhang S Q, Liang Y C. Negative effects of oxytetracycline on wheat (Triticum aestivum L.) growth, root activity, photosynthesis and chlorophyll contents. Agricultural Sciences in China, 2011, 10(10): 545-553.

[8]Li Z J, Xie X Y, Zhang S Q, Liang Y C. Wheat growth and photosynthesis as affected by oxytetracycline as a soil contaminant. Pedosphere, 2011, 21(2): 244-250.

[9]Hu Z H, Liu Y L, Chen G W, Gui X Y, Chen T H, Zhan X M.Characterization of organic matter degradation during composting of manure–straw mixtures spiked with tetracyclines. Bioresource Technology, 2011, 102(15): 7329-7334.

[10]张树清, 张夫道, 刘秀梅, 王玉军, 张建峰. 高温堆肥对畜禽粪中抗生素降解和重金属钝化的作用. 中国农业科学, 2006, 39(2): 337-343.

Zhang S Q, Zhang F D, Liu X M,Wang Y J, Zhang J F. Degradation of antibiotics and passivation of heavy metals during thermophilic composting process. Scientia Agricultura Sinica, 2006, 39(2): 337-343. (in Chinese)

[11]Djogbenou C I V, 李有建, 廖新俤, 胡博, 吴银宝. 低碳氮比对蛋鸡粪与蔗渣好氧堆肥的影响. 中国家禽, 2011, 33(3): 9-13.

Djogbenou C I V, Li Y J, Liao X F, Hu B, Wu Y B. Effect of low C/N ratio on composting of layer manure using cane trash as bulking agent. China Poultry, 2011, 33(3): 9-13. (in Chinese)

[12]李英军, 何小松. 鸡粪堆肥水溶性有机物转化特征研究. 环境工程学报, 2010, 4(9): 2135-2140.

Li Y J, He X S. Study on characteristics of dissolved organic matter during composting of chicken manure. Chinese Journal of Environmental Engineering, 2010, 4(9): 2135-2140. (in Chinese)

[13]马开星, 邹长明, 赵建荣. 鸡粪堆肥腐熟过程中腐熟度参数的变化. 中国农学通报, 2011, 27(3): 289-292.

Ma K X, Zou C M, Zhao J R. Changes of maturity indexes during the chicken manure composting. Chinese Agricultural Science Bulletin, 2011, 27(3): 289-292. (in Chinese)

[14]郑瑞生, 封辉, 戴聪杰, 李延. 碳氮比对堆肥过程NH3挥发和腐熟度的影响. 环境污染与防治, 2009, 31(9): 59-63.

Zheng R S, Feng H, Dai C J, Li Y. The influence of C/N ratio on NH3 emission and compost maturity. Environmental Pollution & Control, 2009, 31(9): 59-63. (in Chinese)

[15]范寰, 梁军锋, 赵润, 张金凤, 张洪生. 碳氮源对复合木质素降解菌木质素降解能力及相关酶活的影响. 农业环境科学学报, 2010, 29(7): 1394-1398.

Fan H, Liang J F, Zhao R, Zhang J F, Zhang H S. Effect of carbon and nitrogen sources on lignin degradation ability and enzyme activity of dual-cultured lignin-degrading fungi. Journal of Agro-Environment Science, 2010, 29(7): 1394-1398. (in Chinese)

[16]王玉军, 窦森, 张晓梅, 李文朋. 堆肥对有机氯农药挥发和降解的效果. 农业工程学报, 2009, 25(12): 265-269.

Wang Y J, Du S, Zhang X M, Li W P. Effect of composting on volatilization and degradation of organo-chlorine pesticide. Transactions of the Chinese Society of Agricultural Engineering, 2009, 25(12): 265-269. (in Chinese)

[17]沈颖, 魏源送, 郭睿, 徐超一, 张朝晖, 周光漪, 赵鑫, 刘俊新. 超高效液相色谱串联质谱检测猪粪中残留的四环素类抗生素. 环境化学, 2009, 28(5): 747-752.

Chen Y, Wei Y S, Guo R, Xu C Y, Zhang Z H, Zhou G Y, Zhao X, Liu J X. Determination of tetracyclines residues in swine manure by UPLC/MS. Environmental Chemistry, 2009, 28(5): 747-752. (in Chinese)

[18]Shen Y, Wei Y S, Zheng J X, Fang Y, Chen L P. Biodegradation of tetracycline antibiotics residues in swine manure. The Chinese Journal of Process Engineering, 2009, 9(5): 962-968.

[19]中国科学院南京土壤研究所. 土壤理化分析. 上海: 上海科学技术出版社, 1978: 1-250.

Nanjing Soil Research Institute of China Academy of Science. Soil Physics and Chemics Analysis. Shanghai: Shanghai Science and Technology Press, 1978: 1-250. (in Chinese)

[20]Ivone V M, Maria E S, Celia M M, Olga C N. Diversity of bacterial isolates from commercial and homemade composts. Microbial Ecology, 2008, 55(4): 714-722.

[21]Zucconi F, Forte M, Monac A, De Bertoldi M. Biological evaluation of compost maturity. Biocycle, 1981, 22(4): 27-29.

[22]Selvam A, Zhao Z Y, Wong J W C. Composting of swine manure spiked with sulfadiazine, chlortetracycline and ciprofloxacin. Bioresource Technology, 2012, 126: 412-417.

[23]De Liguoro M, Cibin V, Capolongo F, Halling-Sørensen B, Montesissa C. Use of oxytetracycline and tylosin in intensive calf farming: evaluation of transfer to manure and soil. Chemosphere, 2003, 52(1): 203-212.

[24]Jiao S J, Zheng S R, YIN D Q, Wang L H, Chen L Y Aqueous oxytetracycline degradation and the toxicity change of degradation compounds in photoirradiation process. Journal of Environmental Sciences, 2008, 20(7): 806-813.

[25]Hu Z H, Liu Y L, Chen G W, Gui X Y, Chen T H, Zhan X M . Characterization of organic matter degradation during composting of manure-straw mixtures spiked with tetracyclines. Bioresource Technology, 2011, 102(15): 7329-7334.

[26]Kim K R, Owens G, Ok Y S, Park W K, Lee D B, Kwon S I. Decline in extractable antibiotics in manure-based composts during composting. Waste Management, 2012, 32(1): 110-116.

[27]Liu R, Huang X, Sun Y F, Qian Y. Hydrodynamic effect on sludge accumulation over membrane surfaces in a submerged membrane bioreactor. Process Biochemistry, 2003, 39(2): 157-163.

[28]Ratasuk N, Boonsaner M, Hawker D W. Effect of temperature, pH and illumination on abiotic degradation of oxytetracycline in sterilized swine manure. Journal of Environmental Science and Health, Part A, 2012, 47(11): 1687-1694.

[29]Zhu N W. Effect of low initial C/N ratio on aerobic composting of swine manure with rice straw. Bioresource Technology, 2007, 98(1): 9-13.

[30]Liang C, Das K C, McClendon R W. The influence of temperature and moisture contents regimes on the aerobic microbial activity of a biosolids composting blend. Bioresource Technology, 2003, 86(2): 131-137.

[31]Huang G F, Wong J W C, Wu Q T, Naga B B. Effect of C/N on composting of pig manure with sawdust. Waste Management, 2004, 24(8): 805-813.

[32]Sundberg C, Smårs S, Jönsson H. Low pH as an inhibiting factor in the transition from mesophilic to thermophilic phase in composting. Bioresource Technology, 2004, 95(2): 145-150.

[33]Hang R T. The Practical Handbook of Compost Engineering. USA: Lewis Publishers, 1993: 201.

[34]Jimenez E I, Garcia V P. Composting of domestic refuse and sewage sludge. I. Evolution of tempetature, pH, C/N ratio and cation- exchange capacity. Resouse, Conservation and Recycling, 1991, 6(1): 45-60.

[35]Kong W D, Zhu Y G, Fu B J, Marschner P, He J Z. The veterinary antibiotic oxytetracycline and Cu influence functional diversity of the soil microbial community. Environmental Pollution, 2006, 143(1): 129-137.

[36]Arikan O, Walter M, David I, Patricia M. Minimally managed composting of beef manure at the pilot scale: Effect of manure pile construction on pile temperature profiles and on the fate of oxytetracycline and chlortetracycline. Bioresource Technology, 2009, 100(19): 4447-4453.

[37]刘伟, 王慧, 陈小军, 杨大为, 匡光伟, 孙志良. 抗生素在环境中降解的研究进展. 动物医学进展, 2009, 30(3): 89-94.

Liu W, Wang H, Chen X J, Yang D W, Kuang G W, Sun Z L. Progress on degradation of antibiotics in environment. Progress in Veterinary Medicine, 2009, 30(3): 89-94. (in Chinese)

[38]张相锋, 王洪涛, 周辉宇, 张义安, 聂永丰. 花卉废物和牛粪联合堆肥中的氮迁移. 环境科学, 2003, 24(3): 126-131.

Zhang X F, Wang H T, Zhou H Y, Zhang Y A, Nie Y F. Nitrogen transformation during co-composting of flower wastes and cattle manure. Environmental Science, 2003, 24(3): 126-131. (in Chinese)

[39]Zucconi F, Pera A, Forte M, De Bertoldi M. Evaluating toxicity of immature compost. Biocycle, 1981, 22(2): 54-57.

[40]钱学玲, 孙义, 李道棠. 模糊综合评价法判别堆肥腐熟度的研究. 上海环境科学, 2001, 20(2): 84-87.

Qian X L, Sun Y, Li D T. Study on degree of maturity of compost using fuzzy comprehensive assessment. Shanghai Environmental Sciences, 2001, 20(2): 84-87. (in Chinese)