QuEChERS前处理结合HPLC-MS/MS法分析氯虫苯甲酰胺在甘蓝和土壤中的残留

doi:10.3864/j.issn.0578-1752.2012.13.008

摘要/Abstract

摘要： 【目的】研究氯虫苯甲酰胺在蔬菜上和土壤中的残留降解动态，制定氯虫苯甲酰胺制剂防治蔬菜害虫的最佳施用量和安全间隔期。【方法】采用QuEChERS前处理方法结合高效液相色谱-串联质谱（HPLC-MS/MS）测定20%氯虫苯甲酰胺悬浮剂中氯虫苯甲酰胺在甘蓝和土壤中的残留降解动态和最终残留量。【结果】氯虫苯甲酰胺在甘蓝和土壤中的添加回收率分别为81.25%—92.05%和82.92%—93.38%；HPLC-MS/MS定性分析表明甘蓝和土壤中的残留物质为氯虫苯甲酰胺。氯虫苯甲酰胺在甘蓝和土壤中降解动态符合一级动力学指数模型，在甘蓝上和土壤中的半衰期分别为7.66和6.86 d。20%氯虫苯甲酰胺悬浮剂以0.045 g•m-2施药时，它在甘蓝和土壤中的最终残留浓度分别为未检出和0.0071 mg•kg-1；0.090 g•m-2剂量施药时，它在甘蓝和土壤中的最终残留浓度分别为0.0063和0.1004 mg•kg-1。【结论】20%氯虫苯甲酰胺悬浮剂以0.045和0.090 g•m-2剂量施药时，氯虫苯甲酰胺在甘蓝和土壤中的最终残留浓度符合残留要求，可以安全使用。

关键词: 氯虫苯甲酰胺, QuEChERS, HPLC-MS/MS, 降解, 残留

Abstract: 【Objective】 The objective of this study is to research degradation dynamics of chlorantraniliprole in the vegetable and soil, and to formulate a scientific basis for the reasonable spray volume and the safe interval period on controlling vegetable pests.【Method】Degradation dynamics and terminal residue of chlorantraniliprole of 20% chlorantraniliprole SC in cabbage and soil were investigated by QuEChERS-high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS).【Result】 The recoveries of chlorantraniliprole from the cabbage and the soil were 81.25%-92.05% and 82.92%-93.38%, respectively. The residues in the cabbage and soil were proved to chlorantraniliprole through qualitative analysis by HPLC-MS/MS. Degradation dynamic equations of chlorantraniliprole in cabbage and soil fitted the first-order reaction dynamic equations. The half-lives of degradation of chlorantraniliprole in cabbage and soil were 7.66 and 6.86 d, respectively. The final residues of chlorantraniliprole were not detected in cabbage and 0.0071 mg•kg-1 in soil, respectively, when 20% chlorantraniliprole SC was applied at 0.045 g•m-2. The final residues of chlorantraniliprole were 0.0063 mg•kg-1 in cabbage and 0.1004 mg•kg-1 in soil, respectively, when 20% chlorantraniliprole SC was applied at 0.090 g•m-2.【Conclusion】20% chlorantraniliprole SC was applied at 0.045 and 0.090 g•m-2 could be considered safe in the cabbage field.

Key words: chlorantraniliprole, QuEChERS, HPLC-MS/MS, degradation, residue

陈小军, 王萌, 范淑琴, 崔海荣, 杨益众. QuEChERS前处理结合HPLC-MS/MS法分析氯虫苯甲酰胺在甘蓝和土壤中的残留[J]. 中国农业科学, 2012, 45(13): 2636-2647.

CHEN Xiao-Jun, WANG Meng, FAN Shu-Qin, CUI Hai-Rong, YANG Yi-Zhong. Degradation Dynamics and Residues Analysis of Chlorantraniliprole in Cabbage and Soil by QuEChERS-High Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Scientia Agricultura Sinica, 2012, 45(13): 2636-2647.

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