利用微卫星多态性揭示酿酒酵母菌株遗传多样性

doi:10.3864/j.issn.0578-1752.2012.12.024

摘要/Abstract

摘要： 【目的】利用微卫星标记技术对来自新疆和宁夏的48株酿酒酵母菌株进行区分，并分析其亲缘关系。【方法】选择4个微卫星位点：SCAAT1、SCYOR267c、C11和YPL009c，用其多态性对48个菌株进行分析。【结果】4个微卫星标记在48株菌中共检测到87个等位基因，39种基因型；4个位点多态信息含量为0.8593—0.9115，均为高度多态位点；期望杂合度（heterozygosity expected, He）和观测杂合度（heterozygosity observed, Ho）分别为0.8803—0.9268和0.8333—0.9792。【结论】宁夏和新疆地区酿酒酵母菌株生物多样性丰富。

关键词: 酿酒酵母, 微卫星标记, 菌株区分, 生物多样性 , , , , , ,

Abstract: 【Objective】A total of 48 Saccharomyces cerevisiae strains from Xinjiang and Ningxia regions were typed by 4 microsatellite markers, and the genetic relations among the strains were also analyzed.【Method】A total of 48 Saccharomyces cerevisiae strains were discriminated for polymorphism at the 4 selected loci: SCAAT1, SCYOR267c, C11, and YPL009c.【Result】A total of 87 alleles, which generated 39 genotypes, were found. The polymorphism information contents (PIC) of all strains at 4 loci were 0.8593-0.9115, suggesting that the 4 SSR loci were hypervariable. The heterozygosity expected and heterozygosity observed were 0.8803-0.9268 and 0.8333-0.9792, respectively.【Conclusion】The results showed the diversity of Saccharomyces cerevisiae strains in Ningxia and Xinjiang regions.

Key words: Saccharomyces cerevisiae, microsatellite markers, strain typing, biodiversity

冯敏, 王春晓, 刘延琳. 利用微卫星多态性揭示酿酒酵母菌株遗传多样性[J]. 中国农业科学, 2012, 45(12): 2537-2543.

FENG Min, WANG Chun-Xiao, LIU Yan-Lin. Genetic Diversity of Saccharomyces cerevisiae Strains Revealed by Microsatellite Sequence Polymorphism[J]. Scientia Agricultura Sinica, 2012, 45(12): 2537-2543.

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