鲁西牛ANGPTL6基因的3个多态位点与其生长性状的关联性分析

doi:10.3864/j.issn.0578-1752.2012.11.021

摘要/Abstract

摘要： 【目的】揭示鲁西牛ANGPTL6 (angiopoietin-like protein 6)基因的遗传变异特征，发现与鲁西牛生长性状显著相关的候选分子标记，为鲁西牛分子育种积累基础资料。【方法】以183头鲁西牛血样为材料，运用DNA池测序和PCR-RFLP等技术检测ANGPTL6基因的序列变异。【结果】检测到鲁西牛ANGPTL6基因内含子中3个新的SNPs。χ2检验表明这3个多态位点均处于哈代-温伯格平衡状态（P＞0.05）。遗传变异特征分析显示，T2359C 和 G3258T位点属于中度多态性，C2403A位点属于低度多态性。连锁不平衡和单倍型分析表明，鲁西牛ANGPTL6基因3个多态位点之间为弱连锁，单倍型TCG（野生型）属于优势单倍型（频率为44.3%）。方差分析表明，单个的SNP和不同SNPs之间的组合均与鲁西牛生长性状有着显著或极显著的关联，杂合型个体的生长性状指标均高于纯合型个体。【结论】鲁西牛ANGPTL6基因的3个SNPs与其生长性状显著相关，并且杂合基因型个体明显优于纯合基因型个体，此结果可以应用于鲁西牛的分子育种实践。

关键词: ANGPTL基因, 多态性, 关联性, 生长性状, 鲁西牛

Abstract: 【Objective】 The purpose of this study is to reveal the genetic variation characteristics of bovine ANGPTL6 gene and find several candidate molecular markers that related to growth traits of Luxi cattle, thus for accumulating some basic information for molecular breeding of Luxi cattle breed.【Method】As investigative materials blood samples collected from 183 Luxi cows were used and sequence variants within ANGPTL6 gene were detected by DNA pool sequencing and PCR-RFLP technology.【Result】The results showed that three novel SNPs within the intron region of ANGPTL6 gene were identified in Luxi cattle breed. χ2 test showed that three loci of the Luxi cattle population were all in Hardy-Weinberg equilibrium (P＞0.05). Analysis of genetic diversity indicated T2359C and G3258T loci were at intermediate polymorphic status, but C2403A locus was at low polymorphic status. Further, the linkage disequilibrium and haplotypes were analyzed among three SNPs, suggested that they are all little linked. Interestingly, haplotype of TCG (wild-type) was dominant (with frequency of 44.3%). The variance analysis showed that the different genotypes of single and combined site were all significantly or extremely significantly associated with growth traits of Luxi cattle, and the growth traits of heterozygous individuals were all higher than the homozygous individuals.【Conclusion】The three novel SNPs are all significantly related to growth traits of Luxi cattle, and the heterozygous individuals are all more superior than the homozygous individuals, which suggested these results could apply to molecular breeding industry of Luxi cattle.

Key words: ANGPTL6 gene, polymorphism, association, growth trait, Luxi cattle

李爱民, 马云, 杨东英, 蓝贤勇, 胡沈荣, 雷初朝, 张春雷, 陈宏. 鲁西牛ANGPTL6基因的3个多态位点与其生长性状的关联性分析[J]. 中国农业科学, 2012, 45(11): 2306-2314.

LI Ai-Min, MA Yun, YANG Dong-Ying, 蓝Xian-Yong , HU Shen-Rong, LEI Chu-Chao, ZHANG Chun-Lei, CHEN Hong. Association Analysis of Three SNPs Within ANGPTL6 Gene with Growth Traits in Luxi Cattle[J]. Scientia Agricultura Sinica, 2012, 45(11): 2306-2314.

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