苏淮猪抗腹泻MUC13基因rs319699771位点多态性 及其与经济性状的关联

doi:10.3864/j.issn.0578-1752.2019.08.014

中国农业科学 ›› 2019, Vol. 52 ›› Issue (8): 1449-1457.doi: 10.3864/j.issn.0578-1752.2019.08.014

• 畜牧·兽医·资源昆虫 • 上一篇下一篇

苏淮猪抗腹泻MUC13基因rs319699771位点多态性及其与经济性状的关联

刘晨曦^1,²,王彬彬^1,²,蒲广^1,²,张倩^1,²,曹旸^1,²,王欢^1,²,高琛^1,²,牛培培^2,³,李平华^1,^2,³(),黄瑞华^1,^2,³()

1 南京农业大学养猪研究所/动物科技学院,南京 210095
2 南京农业大学淮安研究院,江苏淮安 223005
3 淮安市南农大新农村发展研究有限公司,江苏淮安 223005

收稿日期:2018-09-20 接受日期:2019-01-21 出版日期:2019-04-16 发布日期:2019-04-26
通讯作者: 李平华,黄瑞华
作者简介:刘晨曦,E-mail：2577797585@qq.com。
基金资助:
国家自然基金(31601923);国家自然基金(31672381);淮安市重点研发计划(HAN201618);淮安市重点研发计划(HAN201617);江苏省农业三新工程项目(SXGC [2017] 275);畜禽舍内氨气浓度监测系统及控制设备的研发(NSY2080003);南京农业大学淮安研究院建设(BM2017020);“家庭牧场环境优化技术集成与推广”项目(SXGC[2016]146)

Polymorphism of Rs319699771 Locus of Anti-Diarrhea MUC13 Gene in Suhuai Pig Population and Their Association with Economic Traits

LIU ChenXi^1,²,WANG BinBin^1,²,PU Guang^1,²,ZHANG Qian^1,²,CAO Yang^1,²,WANG Huan^1,²,GAO Chen^1,²,NIU PeiPei^2,³,LI PingHua^1,^2,³(),HUANG RuiHua^1,^2,³()

1 Institute of Swine Science, Nanjing Agricultural University, Nanjing 210095
2 Huaian Academy of Nanjing Agricultural University, Huaian Jiangsu 223005
3 Huaian New Rural Development Co. LTD of Nanjing Agricultural University, Huaian Jiangsu 223005

Received:2018-09-20 Accepted:2019-01-21 Online:2019-04-16 Published:2019-04-26
Contact: PingHua LI,RuiHua HUANG

摘要/Abstract

摘要：

【背景】MUC13基因是调控致使仔猪断奶前腹泻的产肠毒素大肠杆菌F4ac感染的主效基因,该基因上rs319699771位点（G/A突变）能准确鉴别易感和抗性个体,其中GG型是抗腹泻基因型,苏淮猪抗腹泻性能分子选育是通过选留GG型个体来实现。但在选留抗腹泻GG型个体时是否会对苏淮猪其它重要经济性状如生长、胴体、肉质产生不利影响尚未明晰。【目的】旨在分析该位点是否与其它性状关联,以确定基于MUC13基因rs319699771位点抗腹泻性能的分子选育是否会对苏淮猪其它经济性状产生不利影响。【方法】以313头体重为87.61±0.54 kg的苏淮育肥猪为试验动物,屠宰测定其胴体和肉质性状,以261头日龄为161.1±0.5 d苏淮后备猪为试验动物,测定其生长、体尺性状,同时采集相应苏淮育肥猪和后备猪群耳组织样提取组织DNA,经过多重PCR反应进行MUC13基因rs319699771位点多态性检测,采用SAS软件中一般线性模型分析MUC13基因rs319699771位点多态性基因型和苏淮猪肉质、胴体和生长性状的关联性。【结果】MUC13基因rs319699771位点多态性检测结果显示,苏淮猪育肥群公、母猪群体MUC13基因rs319699771位点的抗腹泻G等位基因频率分别为0.695和0.634,抗腹泻优势GG型频率在苏淮猪育肥群公、母群体中分别为0.467和0.373;该位点抗腹泻G等位基因频率在苏淮后备群公、母猪群体分别为0.690和0.705,抗腹泻优势GG型频率在后备群公、母猪群体中分别为0.508和0.480,说明苏淮猪抗腹泻等位基因频率较高,通过分子选育提升苏淮猪抗腹泻GG型频率的可行性强。MUC13基因rs319699771位点多态性与苏淮猪经济性状关联分析结果显示：育肥猪群体该位点多态性与胴体、肉质性状均无显著关联（P>0.05）,可见在苏淮猪育肥猪群体内加大对抗腹泻MUC13基因rs319699771位点的选育不会影响到苏淮育肥猪的胴体和肉质性状;苏淮后备猪群体该位点多态性与腿臀围指标存在极显著关联（P<0.01）,该位点GG型个体腿臀围平均比比AG型个体腿臀围长1.46 cm,比AA型个体腿臀围长3 cm;与日增重和结测体重的关联性分析有关联趋势（P<0.10）,GG型个体相较于AA、AG型个体有提升趋势,对于这三个性状来说,有利基因型都是GG型,说明选留该位点GG型进行苏淮后备猪抗腹泻选育的同时还可以提升苏淮后备猪生长相关性状。【结论】据此,苏淮猪群体MUC13基因rs319699771位点抗腹泻等位基因频率较高,进行抗腹泻选育的可行性强,同时对于苏淮猪群体,不仅可通过选留提升MUC13基因rs319699771位点GG型频率来提高抗腹泻能力,还能同时实现对苏淮猪群腿臀围、日增重的选育提升。

关键词: 苏淮猪, MUC13基因, 抗腹泻, 经济性状, 关联性分析

Abstract:

【Background】 MUC13 gene is one of the main genes, which can regulate the infection of enterotoxigenic Escherichia coli F4ac, and cause diarrhea in piglets before weaning. The rs319699771 locus of the gene (G/A mutation) can accurately identify susceptible and resistant individuals, among which GG type is an anti-diarrhea genotype. The anti-diarrhea molecular breeding of Suhuai pig has been implemented by selecting GG genotype individuals, but whether the selection of GG genotype individuals will have adverse effects on other important economic traits, such as growth, carcass and meat quality, which is not yet clear. 【Objective】 The purpose of this study was to analyze the association between the locus and other economic traits to determine whether the molecular selection based on the rs319699771 locus of anti-diarrhea MUC13 gene would have adverse effects on other economic traits of Suhuai pig. 【Method】 In this experiment, 313 Suhuai fattening pigs weighting 87.61±0.54 kg were tested as experimental animals, and their carcass and meat quality traits were determined through slaughter. 261 Suhuai gilt pigs aging for 161.1±0.5 d were tested as experimental animals, and their growth and body size phenotype were also determined. At the same time, the corresponding ear tissue samples of Suhuai fattening pigs and gilt pigs were collected to extract tissue DNA. After Multiplex-PCR reactions, the polymorphism of the rs319699771 locus of MUC13 gene was detected in each pig. The association analysis between the polymorphism genotype of the rs319699771 locus of MUC13 gene and meat quality, carcass and growth traits were conducted by using the general linear model in SAS software. 【Result】 The results of polymorphism of rs319699771 locus of MUC13 gene showed that among the frequency of anti-diarrhea G allele of rs319699771 locus of MUC13 gene, the male and female pigs of the fattening pigs reached 0.695 and 0.634, respectively, and the anti-diarrhea GG genotype frequency was 0.467 and 0.373, respectively, in the male and female fattening pigs. While the locus G allele among the male and female pigs of the gilt reached 0.690 and 0.705, respectively, and the anti-diarrhea GG genotype frequency was 0.508 and 0.480, respectively, in the male and female gilt pigs. This indicated that the frequency of anti-diarrhea gene in Suhuai pig belonged to a higher level, and it could be feasible for the further improvement of anti-diarrhea GG frequency in the Suhuai pig through molecular breeding. The association analysis between polymorphism of rs319699771 locus of MUC13 gene and economic traits of Suhuai pig showed that there was no significant association between the locus’s polymorphism and carcass, meat quality traits in the fattening pigs (P>0.05), and it could be seen that increasing the breeding of the rs319699771 locus of MUC13 gene in Suhuai fattening pigs would not affect the carcass and meat quality traits. The locus’s polymorphism in the gilt extremely associated with the ham circumference index (P<0.01), and on average, the ham circumference of GG type individuals at this locus was about 1.46 cm longer than that of AG type individuals, and about 3 cm longer than that of AA type individuals. Also, the locus tended to associate with daily gain and terminal measurement weight (P<0.10), GG type individuals showed an upward trend compared with AA and AG type individuals. All of the three traits, GG genotype was a favorable genotype. The results showed that the breeding of the anti-diarrhea GG genotype of this locus could improve the growth traits of Suhuai gilt pigs. 【Conclusion】 Based on these results, the anti-diarrhea allele frequency of the rs319699771 locus of MUC13 gene in Suhuai pig was high, and the anti-diarrhea breeding was feasible, and the anti-diarrhea ability of Suhuai pig could be improved by selecting and remaining resistant homozygous GG genotype of rs319699771 locus of MUC13 gene and raising the frequency of the resistant homozygous genotype. At the same time, it could also realize the improvements of ham circumference and daily gain of Suhuai pig.

Key words: Suhuai pig, MUC13 gene, anti-diarrhea, economic traits, association analysis

刘晨曦,王彬彬,蒲广,张倩,曹旸,王欢,高琛,牛培培,李平华,黄瑞华. 苏淮猪抗腹泻MUC13基因rs319699771位点多态性及其与经济性状的关联[J]. 中国农业科学, 2019, 52(8): 1449-1457.

LIU ChenXi,WANG BinBin,PU Guang,ZHANG Qian,CAO Yang,WANG Huan,GAO Chen,NIU PeiPei,LI PingHua,HUANG RuiHua. Polymorphism of Rs319699771 Locus of Anti-Diarrhea MUC13 Gene in Suhuai Pig Population and Their Association with Economic Traits[J]. Scientia Agricultura Sinica, 2019, 52(8): 1449-1457.

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[33]	BERGLUND B . Genetic improvement of dairy cow reproductive performance. Reproduction in Domestic Animals, 2010, 43(s2):89-95.

MUC13基因 MUC13 gene	引物序列 Primer sequence
PCR引物序列 PCR primer sequence	上游 Upstream	TCCTCACACCTCCTGCCTTTTC
PCR引物序列 PCR primer sequence	下游 Downstream	TCAAAACAGAGCGATTTCTGGGTAA
多重PCR连接引物序列 Multiplex-PCR binding primer sequence	MUC13_A_GFP	TGAATTTTGGCTATAGRCCTGTGGTTTTTTTTTTTTTTTTTTTTT
	MUC13_A_GFG	TTCCGCGTTCGGACTGATATTCCATGTACATTTCAGAGTCTGAGGGTTG
	MUC13_A_GFA	TACGGTTATTCGGGCTCCTGTTCCATGTACATTTCAGAGTCTGAGGGCTA

群体 Group	性别 Gender (n)	基因型频率Genotype frequency			基因频率Gene frequency
群体 Group	性别 Gender (n)	AA (n)	AG (n)	GG (n)	A	G
育肥群 Fattening pigs	母猪Sow (67)	0.104 (7)	0.522 (35)	0.373 (25)	0.366	0.634
	阉公猪Barrow (246)	0.077 (19)	0.455 (112)	0.467 (115)	0.305	0.695
	合计Total (313)	0.083 (26)	0.470 (147)	0.447 (140)	0.318	0.682
后备群 Gilt	母猪Sow (198)	0.071 (14)	0.449 (89)	0.480 (95)	0.295	0.705
	公猪Boar (63)	0.127 (8)	0.365 (23)	0.508 (32)	0.310	0.690
	合计Total (261)	0.084 (22)	0.429 (112)	0.487 (127)	0.299	0.701

基因型 Genotype	个体数 Number	胴体性状Carcass performances
基因型 Genotype	个体数 Number	胴体重 Weight of carcass (kg)	肋骨数 Number of ribs	胴体直长 Straight length of carcass (cm)	胴体斜长 Oblique length of carcass (cm)	6-7肋膘厚 Backfat thickness at 6-7 ribs (mm)	肩部最厚处膘厚 Backfat thickness at the thickest part of the shoulder (mm)	最后肋骨膘厚 Backfat thickness at the last rib (mm)	腰荐结合膘厚 Backfat thickness at the lumbosacral junction (mm)	三点平均背膘厚 Average backfat thickness (mm)
AA	26	55.89±1.45	30.33±0.75	88.14±0.75	73.97±0.69	29.17±1.26	34.11±1.28	20.06±1.08	22.48±1.40	25.56±1.11
AG	147	57.51±0.70	29.66±0.36	88.69±0.36	74.00±0.33	28.46±0.61	34.15±0.62	21.12±0.52	23.74±0.67	26.37±0.54
GG	140	57.35±0.71	29.82±0.37	88.60±0.37	73.96±0.34	28.87±0.61	34.45±0.63	21.93±0.53	23.59±0.68	26.67±0.54
P-value		0.5470	0.6778	0.7754	0.9955	0.7797	0.9064	0.1684	0.6795	0.6106

基因型 Genotype	个体数 Number	肉质性状Meat quality performances
基因型 Genotype	个体数 Number	2h亮度值 L_2h lightness	2h红度值 a_2h redness	2h黄度值 b_2h yellowness	24h亮度值 L_24h lightness	24h红度值 a_24h redness	24h黄度值 b_24h yellowness	pH_45min	pH_24h	滴水损失 Drip loss (%)	系水力 Water holding capacity (%)	肌内脂肪含量 IMF (%)
AA	26	39.71±0.69	5.10±0.30	11.50±1.67	44.31±0.77	5.76±0.30	12.36±0.26	6.43±0.06	5.65±0.04	2.41±0.24	24.83±1.09	2.07±0.15
AG	147	41.18±0.33	4.66±0.15	11.51±0.81	46.00±0.37	5.51±0.14	12.35±0.13	6.37±0.03	5.67±0.02	2.27±0.12	25.47±0.53	1.98±0.07
GG	140	41.16±0.34	4.44±0.15	12.46±0.81	46.03±0.37	5.48±0.14	12.51±0.13	6.43±0.03	5.70±0.02	2.54±0.12	24.84±0.53	1.99±0.07
P-value		0.1034	0.0843	0.5849	0.0800	0.6711	0.5332	0.2726	0.4739	0.1345	0.5645	0.8144

基因型 Genotype	个体数 Number	生长性状Growth performances
基因型 Genotype	个体数 Number	日增重 Daily gain (g·d^-1)	结测体重 Terminal measurement weight (kg)	体长 Body length (cm)	体高 Body height (cm)	胸围 Chest circumference (cm)	腹围 Abdominal circumference (cm)	管围 Cannon bone circumference (cm)	腿臀围 Ham circumference (cm)	活体背膘 Live backfat (mm)	活体眼肌面积 Live loin eye area (cm²)
AA	22	347.87±9.09	56.50±1.47	97.65±1.29	53.06±0.97	91.31±1.12	102.54±1.91	15.13±0.24	72.78±0.99^b	11.79±0.66	17.18±4.76
AG	112	362.89±4.33	58.43±0.07	98.30±0.61	53.53±0.46	92.05±0.54	103.83±0.91	15.31±0.11	74.32±0.47^ab	11.78±0.31	20.84±2.27
GG	127	370.48±4.12	59.62±0.67	99.20±0.58	54.44±0.44	91.14±0.52	102.33±0.87	15.22±0.11	75.78±0.45^a	11.73±0.03	22.03±2.16
P-value		0.0514	0.0601	0.1784	0.1942	0.4347	0.4209	0.7086	0.0045	0.9917	0.6279

苏淮猪抗腹泻MUC13基因rs319699771位点多态性及其与经济性状的关联

Polymorphism of Rs319699771 Locus of Anti-Diarrhea MUC13 Gene in Suhuai Pig Population and Their Association with Economic Traits

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苏淮猪抗腹泻MUC13基因rs319699771位点多态性 及其与经济性状的关联

Polymorphism of Rs319699771 Locus of Anti-Diarrhea MUC13 Gene in Suhuai Pig Population and Their Association with Economic Traits

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苏淮猪抗腹泻MUC13基因rs319699771位点多态性及其与经济性状的关联