利用类番茄茄LA2951渐渗系群体鉴定番茄抗晚疫病QTL

doi:10.3864/j.issn.0578-1752.2012.06.007

摘要/Abstract

摘要： 【目的】利用来自番茄近缘野生种类番茄茄（Solanum lycopersicoides）LA2951的渐渗系群体，挖掘LA2951中潜在的抗晚疫病基因。【方法】采用离体叶片接种法，每片小叶背面接种10 μL菌液（2×104孢子囊/mL），在温度19℃和相对湿度70%—100%的条件下，第6天测量叶片病斑面积（lesion size，LS）和发病率（disease incidence，DI）。【结果】LA2951对晚疫病的抗性受QTL （quantitative trait loci）控制。接种番茄晚疫病T1,2小种，鉴定出Rpiq1b、Rpiq2b、Rpiq4b、Rpiq8a和Rpiq11等5个QTL，可显著减少叶片病斑面积；Rpiq1a、Rpiq2a和Rpiq8b等3个QTL，可明显降低叶片的发病率。接种致病力较强的小种T1,2,4，只鉴定出2个可减少DI的QTL（Rpiq4a和Rpiq5）。说明来自LA2951的QTL呈现明显的小种特异抗性。根据番茄高密度遗传图谱，本研究鉴定的QTL与前人鉴定出的番茄抗晚疫病QTL均同位。另外，无论是接种T1,2小种还是T1,2,4小种，均发现2个感病位点（Spiq4和Spiq10），它们分别位于第4条和第10条染色体短臂末端，可显著增加番茄叶片的DI。【结论】定位了来自类番茄茄LA2951的10个抗晚疫病QTL和2个感病QTL，抗病QTL呈现明显的小种特异抗性，研究结果为番茄抗晚疫病育种提供了理论参考。

关键词: 类番茄茄, 渐渗系, 晚疫病, 数量性状位点

Abstract: 【Objective】The objective of this study is to explore the potential loci for resistance to late blight of introgression line (IL) population derived from a nightshade related species Solanum lycopersicoides LA2951. 【Method】 Detached-leaflet assay (DLA) was used for the artificial inoculation. The abaxial surface of each leaflet was inoculated with a drop of 10 μL sporangial suspension (2×104 sporangiums/mL), and the inoculated leaves were incubated at 19℃ with 70%-100% relative humidity. Lesion size (LS) of the leaf and disease incidence (DI) were measured 6 d after inoculation.【Result】The resistance of this wild species was controlled by quantitative trait loci (QTL). In total, 5 QTLs (Rpiq1b, Rpiq2b, Rpiq4b, Rpiq8a, Rpiq11) were identified after inoculation with race T1,2, which significantly reduced LS, and 3 QTLs (Rpiq1a, Rpiq2a, Rpiq8b) were indentified which clearly reduced DI. However, only 2 QTLs (Rpiq4a and Rpiq5) reducing DI were indentified after inoculation with more virulent race T1,2,4. It proved that QTLs derived from LA2951 showed a clear race-specific resistance. Based on tomato high density genetic linkage map, all QTLs detected in this paper were co-localized with those previously identified in other tomato wild species. In addition, 2 susceptibility QTLs (Spiq4 and Spiq10) increasing DI were identified after inoculation with both race T1,2 and race T1,2,4, which were located at the end of short arm of chromosome 4 and chromosome 10, respectively. 【Conclusion】Ten resistance QTLs and 2 susceptibility QTLs were identified, and they presented a race-specific behavior. The results obtained in this paper provided a theoretical basis for breeding of late blight resistance in tomato.

Key words: Solanum lycopersicoides, introgression line, late blight, quantitative trait loci

张春芝, 刘磊, 孙玉燕, 周龙溪, 杨宇红, 谢丙炎, 李君明. 利用类番茄茄LA2951渐渗系群体鉴定番茄抗晚疫病QTL[J]. 中国农业科学, 2012, 45(6): 1093-1105.

ZHANG Chun-Zhi, LIU Lei, SUN Yu-Yan, ZHOU Long-Xi, YANG Yu-Hong, XIE Bing-Yan, LI Jun-Ming. Identification of QTLs Conferring Resistance to Late Blight in Solanum lycopersicoides LA2951 Introgression Line Population[J]. Scientia Agricultura Sinica, 2012, 45(6): 1093-1105.

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