围产期奶牛 Nod-2 受体表达变化及其对中性粒细胞呼吸爆发功能的影响

doi:10.3864/j.issn.0578-1752.2012.03.018

摘要/Abstract

摘要： 【目的】围产期奶牛对细菌性疾病的易感性升高。本研究的目的在于探讨 Nod-2 受体在围产期奶牛中性粒细胞（PMN）中的表达变化及其对 PMN 细胞呼吸爆发功能的影响，以揭示 Nod-2 在围产期奶牛免疫机能改变中的作用。【方法】采用 RT-PCR 法检测围产期和非围产期泌乳奶牛外周 PMN 细胞中 Nod-2 mRNA 的表达变化；全血分别用佛波醇酯（PMA）和 Nod-2 受体特异性配体胞壁酰基二肽 MDP 刺激，采用流式细胞仪检测 PMN 呼吸爆发功能。【结果】围产期奶牛 PMN 细胞 Nod-2 mRNA 表达量显著低于非围产期奶牛（P＜0.05）；受 PMA 刺激后，围产期奶牛 PMN 呼吸爆发功能显著低于非围产期奶牛（P＜0.05）；受 MDP 刺激后，围产期奶牛 PMN 呼吸爆发功能亦较非围产期奶牛下降。【结论】围产期奶牛对细菌性疾病易感性升高可能与 Nod-2 mRNA 表达下调有关。

关键词: 奶牛, 中性粒细胞, Nod-2 受体

Abstract: 【Objective】 Dairy cows are more susceptible to bacterial diseases around parturition. The present study was conducted to investigate the expression of Nod-2 in the polymorphonuclear neutrophil leukocytes (PMN) from periparturient cows and its effect on PMN respiratory burst, with the purpose to evaluate whether the Nod-2 receptor contributes to the increased susceptibility of periparturient cows to bacterial diseases. 【Method】 The Nod-2 mRNA in PMN from periparturient and non- periparturient cows was detected by RT-PCR. Function of respiratory burst in PMNs was evaluated by flow cytometry following the stimulation of the whole blood with phorbol-12-myristate-13-acetate (PMA) and the ligand of Nod-2, muramyl dipeptide (MDP), respectively. 【Result】 It was found that the blood PMN from periparturient cows had decreased Nod-2 mRNA expression than that from non-periparturient lactating cows (P＜0.05), and their ability of respiratory burst responding to PMA stimulation was decreased (P＜0.05). Upon the MDP stimulation, PMNs from periparturient cows showed decreased respiratory burst as well. 【Conclusion】 It was suggested that the reduced Nod-2 mRNA expression might contribute to the increased susceptibility to bacterial infection in periparturient cows.

Key words: dairy cow, neutrophil, Nod-2 receptor

李文文, 卢政, 谭勋, 李君君. 围产期奶牛 Nod-2 受体表达变化及其对中性粒细胞呼吸爆发功能的影响[J]. 中国农业科学, 2012, 45(3): 556-561.

LI Wen-Wen, LU Zheng, TAN Xun, LI Jun-Jun. Expression of Nod-2 Receptor in Periparturient Dairy Cows and Its Effect on Neutrophils Respiratory Burst[J]. Scientia Agricultura Sinica, 2012, 45(3): 556-561.

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