中国农业科学

• 畜牧·资源昆虫 • 上一篇    下一篇

贵州白山羊和古蔺马羊脂联素基因多态性及其与繁殖性能的关联研究

刘重旭1;王凭青1;张宝云1;储明星2;邓腊梅1;谭颖1;樊奇1   

  1. 1、重庆大学生物工程学院,重庆 400030;
    2、中国农业科学院北京畜牧兽医研究所/农业部畜禽遗传资源与利用重点开放实验室,北京 100193
  • 收稿日期:2010-06-08 出版日期:2011-05-05 发布日期:2010-03-17
  • 通讯作者: 王凭青,Tel:023-65112753;E-mail:wang_pq@21cn.com
  • 作者简介:刘重旭,E-mail:lcx20061110@126.com
  • 基金资助:
    现代农业产业技术体系建设专项(CARS-39)、重庆市自然科学基金重点项目(2009BA1066)

Polymorphism of Adiponectin Gene and Its Relationship with Reproductive Ability in Guizhou White and Gulin Ma Goats

LIUChong-xu;WANGPing-qing;ZHANGBao-yun;CHUMing-xing;DENGLa-mei;TANYing;FANQi   

  1. 1、Bioengineering College, Chongqing University, Chongqing 400030;
    2、Institute of Animal Science, Chinese Academy of Agricultural Sciences/Key Laboratory of Farm Animal Genetic Resources and Utilization of Ministry of Agriculture, Beijing 100193
  • Received:2010-06-08 Online:2011-05-05 Published:2010-03-17

摘要: 【目的】阐明西南地区具有代表性的山羊品种脂联素(adiponectin)基因的多态性及其与繁殖性能之间的关系,为山羊高繁殖力的标记辅助选择提供科学依据。【方法】根据牛的ADIPOQ序列设计4对引物,采用PCR-SSCP技术检测ADIPOQ在川东白山羊、大足黑山羊、贵州白山羊、金堂黑山羊、板角山羊、成都麻羊、南江黄羊、古蔺马羊和马头山羊等9个山羊品种中的单核苷酸多态性,同时在贵州白山羊和古蔺马羊两个群体中研究脂联素基因多态性与山羊繁殖力之间的关系。【结果】4对引物中只有引物P4存在多态性。对于P4的扩增片段,在不同的山羊品种中检测到AA、AG和GG 3种基因型,测序分析表明与AA型相比,GG型有一处单碱基突变(954G→A)。GG型和AG型的贵州白山羊产羔数最小二乘均值分别比AA型的多0.18只(P<0.05)和0.20只(P<0.05);GG型和AG型的古蔺马羊产羔数最小二乘均值分别比AA型的多0.16只(P<0.05)和0.15只(P<0.05)。另外,贵州白山羊和古蔺马羊的不同基因型之间初生重的最小二乘均值均没有显著差异(P>0.05)。【结论】表明ADIPOQ可能是影响贵州白山羊和古蔺马羊多胎性能的一个主效基因或是与之存在紧密连锁的遗传标记。

关键词: 山羊 , 繁殖力 , ADIPOQ , PCR-SSCP

Abstract: 【Objective】The objectives of the present study were to identify the polymorphism of adiponectin gene and its association with reproductive traits in Guizhou White and Gulin Ma goats and to provide a scientific basis for marker-assisted selection for high prolificacy in goats.【Method】According to the sequence of Bos taurus ADIPOQ, four pairs of primers were designed to detect single nucleotide polymorphisms of ADIPOQ in Boer, Chuandong White, Dazu Black, Guizhou White, Jintang Black, Banjiao, Chengdu Ma, Nanjiang Brown, Gulin Ma and Matou goats by PCR-SSCP. The effects of ADIPOQ polymorphisms on the breeding value for litter size and birth weight were examined using least square methods in Guizhou White goats and Gulin Ma goats. 【Result】Only the products amplified by primer P4 displayed polymorphisms of primers P1-P4. For primer P4, three genotypes (AA, AG and GG) were detected in different goat breeds. The sequencing results revealed that the genotype GG had one point mutation (954G→A) compared to AA genotype. The does with genotype GG or AG had 0.18 (P<0.05) or 0.20 (P<0.05) kids more than those with genotype AA in Guizhou White goats, respectively. The does with genotype GG or AG had 0.16 (P<0.05) or 0.15 (P<0.05) kids more than those with genotype AA in Gulin Ma goats, respectively. In addition, Least squares means for birth weight of different ADIPOQ genotypes had no significant difference (P>0.05) in Guizhou White or Gulin Ma goats.【Conclusion】The results preliminarily indicated that the ADIPOQ is either a major gene that influences the prolificacy in Guizhou White and Gulin Ma goats or a molecular marker linked with such a gene closely.

Key words: goat , prolificacy , ADIPOQ , PCR-SSCP