菊花管状花数量和花心直径的QTL分析

doi:10.3864/j.issn.0578-1752.2011.07.017

摘要/Abstract

摘要： 【目的】发掘控制菊花管状花数量和花心直径的主效QTL，为菊花花器性状的分子改良提供参考依据。【方法】以142株菊花‘雨花落英’×‘奥运含笑’F1代群体为材料，调查管状花数量和花心直径两个性状在2008—2009两个年度的分离情况，并基于菊花SRAP遗传图对其进行QTL定位分析。【结果】管状花数量和花心直径在两年间均表现为连续分布，具有数量性状的典型特征，且两者具有极显著的相关性（r=0.37，P＜0.01）。基于菊花SRAP遗传图谱的QTL定位研究共检测到2个QTL与菊花管状花数量显著相关，7个QTL与花心直径显著相关。这9个QTL主要分布在亲本‘雨花落英’遗传图的Y1、Y2和Y21以及‘奥运含笑’遗传图的A5、A13和A19共计6个连锁群上，各个QTL的LOD值介于2.50—4.18，可以解释6.17%—13.72%的表型变异。其中在两年中检测到控制管状花数量的TfnE1Y21和TfnE2Y21以及控制花心直径的FcdE1Y1和FcdE2Y1在连锁群上所处的标记区间相同，应分别属于同一个QTL，受环境的影响较小，其余在单个环境中检测到的QTL受环境影响较大。【结论】共获得9个QTL与菊花管状花数量和花心直径显著相关，其中受环境影响较小的主效QTL可用于菊花分子标记辅助选择育种。

关键词: 菊花 , F1群体 , 管状花数量 , 花心直径 , QTL分析

Abstract: 【Objective】The purpose of this study is to detect main-effect QTL controlling tubular floret number and flower center diameter and provide some reference for molecular marker-assisted breeding of inflorescence traits in chrysanthemum.【Method】In this study, tubular floret number and flower center diameter of chrysanthemum were evaluated in 2008 and 2009, and QTL analysis was undertaken for the two traits based on the two-year phenotypic data in 142 F1 mapping population derived from a cross between chrysanthemum cultivars ‘Yuhualuoying’ and ‘Aoyunhanxiao’ and their SRAP-based genetic linkage maps.【Result】 The two traits of tubular floret number and flower center diameter showed continuous variation and significantly correlated with each other with Pearson correlation coefficient of 0.37 (P＜0.01). The QTL analysis indicated that 2 for tubular floret number and 7 for center floret number QTL were detected based on the two-year phenotypic data. These QTL scattered on 6 linkage groups, including Y1, Y2 and Y3 of ‘Yuhualuoying’ map, and A5, A13 and A19 of ‘Aoyunhanxiao’ map, with a LOD value ranging from 2.50 to 4.18 and a contribution ratio to the phenotypic variation of 6.17%-19.95% for each QTL. TfnE1Y21 and TfnE2Y21 controlling tubular floret number as well as FcdE1Y1 and FcdE2Y1 controlling flower center diameter which were mapped in the same marker interval on the linkage groups of Y21 and Y1 in 2008 and 2009 should be the same QTL and were less affected by environment, respectively. The other QTL detected in either 2008 or 2009 were remarkably affected by environment. 【Conclusion】A total of 9 QTL were mapped for tubular floret number and flower center diameter and the major QTL little affected by environment would be of great significance for the future study of molecular marker assisted breeding program in chrysanthemum.

Key words: chrysanthemum , F1 population , tubular floret number , flower center diameter , QTL analysis

张飞1;2;陈发棣1;房伟民1;陈素梅1;张鲜艳1;尹冬梅1. 菊花管状花数量和花心直径的QTL分析[J]. 中国农业科学, 2011, 44(7): 1443-1450.

ZHANG Fei; CHEN Fa-di; FANG Wei-min; CHEN Su-mei; ZHANG Xian-yan; YIN Dong-mei. QTL Analysis for Tubular Floret Number and Flower Center Diameter of Chrysanthemum[J]. Scientia Agricultura Sinica, 2011, 44(7): 1443-1450.

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